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We narrowed to 466 results for: Sele

Showing: 141 - 160 of 466 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...markers into a target genome. They did this by selecting the important elements from existing transposon...  A new tool for CRISPR gRNA validation Selecting a gRNA sequence that effectively targets your ...sequence or gene of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers...

  2. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...2018 Conference. I don’t exactly know how I was selected to participate on this panel, but my consistent...social media to their professional advantage, I’ve selected five resources that have tips for using social...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...difficult for individual labs to make efficiently. For select viral vectors in the repository, you can instead...in the brain, mapping neuronal networks, and selectively activating signaling pathways. Many scientists...

  4. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...terms in a search bar, by ticking some boxes, by selecting a suggestion — how should these be supported and...viral prep filter for promoter “Syn”, that, when selected, will match variations present in the data such...

  5. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ... contain the RP4 transfer origin as well as a selectable marker and a desired genetic payload (ex: GFP...sorted using FACS and isolated with antibiotic selection. The team tested this system in vitro and in vivo...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...Chemical Biology Find the AsCas12f1 plasmids   Selective endogenous encapsidation for cellular delivery...sequences in the capsid, in an approach called Selective Endogenous eNcapsidation for cellular Delivery...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...production of monoclonal antibodies starts with selecting just one of these B cell clones for further antibody...vials of the same lot of polyclonal antibody, or select a monoclonal or recombinant antibody instead. Otherwise...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... or Cre dependent gene switches, and serotype selection. Promoters and serotype control, respectively,...AAVs down, you’re ready to take the next step – selecting your AAV and AAV components (serotype and promoter...

  9. To Each HIS Own

    Type
    Blog Post
    ...substrates such as Ni2+ and are therefore more selective. When using systems with higher levels of histidine-containing...production systems, consider using these more selective substrates along with stringent wash conditions...

  10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ... gRNA. These gRNAs can be cleaved and rendered useless after less than a minute of exposure to the appropriate...CRISPR resources Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional resources...

  12. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... al., 2013). Ultimately, this allows RABV to selectively infect a genetically defined cell population....RABV transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter...

  14. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... Fig. 2: Schematic of the goldilocks antibody selection process The exposure time of the primary antibody...resources on the Addgene blog Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant...

  16. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...anything dangerous or toxic to humans in bacteria, select “yes” in the drop-down and contact Addgene. We ...prohibit Addgene from distributing the plasmid, select “yes” from the drop-down and add an explanation...

  17. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...fully understood, so it is important to carefully select the target locations of knock-in studies to avoid.../Cas13, which targets RNA instead of DNA and selectively degrades RNA molecules. This system is often ...

  18. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... as well as newly developed calcium sensors. Selecting a calcium sensor Genetically encoded calcium indicators...is a great fit out there waiting for you. When selecting a sensor to use for your studies, you can start...

  19. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...Using PSAMs PSAMs PSAMs, or pharmacologically selective actuator modules, were developed with the goal...specifically and potently activated by pharmacologically selective effector molecules (PSEMs). Higher potency means...

  20. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...includes promoters, UTRs, terminators, etc. Once selected, individual units can be combined into… Transcription...systems as well as additional components such as selectable markers, promoters, enhancers, etc. MoClo is ...
Showing: 141 - 160 of 466 results