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We narrowed to 210 results for: acr.2

Showing: 141 - 160 of 210 results

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...Information Science and Technology 60.4 (2009): 858-862. 2. Van Noorden, Richard. "Interdisciplinary research...Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources on the Addgene Blog 6 Tools...social networking, visual appeal, instant sharing across multiple other sites), I created a new platform...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...intact. First-generation plasmids include (Figure 2): Transfer plasmid — contains transgene and wild-type...envelope removed Envelope plasmid — contains env Figure 2: First-generation lentiviral plasmids. Created with...plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains env Third-generation...The inside out of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: ...Infectivity and Integration Targeting. PLoS ONE , 2 (12), e1340. https://doi.org/10.1371/journal.pone.0001340...features of lentiviral vectors. Human Gene Therapy , 24 (2), 132–142. https://doi.org/10.1089/hum.2012.229 PMID... contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need...

  4. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...is the most charged, with 5 negatively charged and 2 positively charged amino acids. FLAG tag has been ...target-specific antibodies and have been widely used across species and applications. In this blog, we will...been used with moderate to high success rates across all applications. You can find FLAG antibodies on Addgene...

  5. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...interferon gamma (IFNγ) - and eliminate cancer cells. 2. Equilibrium - In a process analogous to the development... 35. Academic Press, 2015. PubMed PMID: 25818339. 2. Curiel, Tyler J., et al. "Specific recruitment of... produces modified dendritic cells (DCs) and macrophages (M1 and M2) that help create welcoming environments...over-express CD47 to escape recognition by circulating macrophages. Tumor cells also downregulate the expression...

  6. Chemogenetics Guide

    Type
    Guide
    ...muscarinic receptor and has not been used in vivo . Figure 2: DREADDs, their ligands, and signaling properties.... Reference Rq(R165L) Human M3 muscarinic Arrestin-2/-3 CNO* Increase Arrestin translocation Arrestin signalling...hM1Dq hM5Dq Human M3 muscarinic G αq CNO* Increase Ca 2+ Neuronal burst firing Armbruster et al., 2007 rM3D...channels, their effect, and outcome in neurons. Table 2. Descriptions of PSAMs and their activity in neurons...Forbes SL, Liggett SB (2001). Modification of the beta 2-adrenergic receptor to engineer a receptor-effector...inhibition Tung et al., 2015 iLMO: GeNL_SS- hGtACR1 GeNL_SS hGtACR1 CFz Cl - influx Neuronal inhibition Ikefuama...

  7. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Activation 95 10 2 1 Denaturation 94 0.5 2 40 Annealing/Extension 60 1 2 40 Enzyme Deactivation 98 10 2 1 Hold ...diploid cells, thus the reason for multiplying by 2. $$V = 2*{copies\ RRE \over copies\ RPP30}$$ Use the viruses...diluted 2-fold serially, the concentration of RRE positive droplets should decrease by a factor of 2 across...should be relatively constant across samples. In the RRE example below, 2-fold serial dilutions of a sample... (Figure 2) below, the concentration of RPP30 positive droplets stays relatively even across samples (...Lentivirus is generally considered biosafety level 2+. Please ensure that you are in compliance with your...channel pipette 200–1000 µL single channel pipette 2–50 µL multichannel pipette 20–200 µL multichannel ...

  8. Western Blot

    Type
    Protocol
    ...transfer, block, incubate with primary antibody Day 2: Incubate with secondary antibody Video Watch this... Microcentrifuge 0.5–10 µL single channel pipette 2–20 µL single channel pipette 20–200 µL single channel...Heat block Mini gel tank chamber Power supply iBlot 2 Gel Transfer Device Roller Spatula Platform shaker...running buffer Prestained protein ladder Ethanol iBlot 2 PVDF Mini Stack, Thermo Fisher IB24002 20X TBS Tween...immediately or store at -80 °C until ready to use. Section 2: Determine the total protein concentration and prepare...in deionized water. To prepare 20% ethanol, dilute 2 mL of ethanol into 8 mL of deionized water and mix...the transfer sandwich as follows: Unseal the iBlot 2 PVDF Mini transfer stack. Set the Top Stack to one...

  9. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...retransduce a model organism such as mice, e.g. first at 2 months, then at 4 months? Or do antibodies become ...reliable quantitation. Differences in sample treatment across methods can affect reported titer by either method...advise that users keep titering method consistent across samples within a project. Targeting Your AAV Q4...

  10. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...double strand break), increasing editing efficiencies 2-3-fold. However, the process of double nicking increases... editing as efficient as possible. Figure 2: Decision tree for selecting a PE6 variant. PE = prime...using paired prime editing. Nature Biotechnology, 40(2), 218–226. https://doi.org/10.1038/s41587-021-01025... 2.3- to 5.1-fold (though up to 45-fold) higher across different genomic sites compared to PE1 (Anzalone...

  11. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...still depolarize neurons at lower MOIs. Figure 2: ChRgers are delivered by intravenous injection into... to perform in vivo optogenetic experiments. Intracranial injections of AAV are commonly used to deliver...neurons and mouse brain slices collected following intracranial injection of an AAV expressing a ChRger. In ...nervous system which eliminates the need for intracranial injections. When packaged in PHP.eB, ChRgers...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...extraction solution, runs the protocol, then uses around 2–4 µl in each 50 µl PCR reaction. The gDNA extracted... early onset Parkinson Point mutations. Cell, 146(2), 318–331. https://doi.org/10.1016/j.cell.2011.06.019...pre-processing form of crRNA is expressed and a tracrRNA (the helper RNA that facilitate the processing... then when expressed in cell and with Cas9 and tracrRNA, it will be processed (i.e., cleaved) into a shorter...form of crRNA is fused chimerically to a mature tracrRNA), the spacer used is only 20 bp. Please refer ...

  13. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...components, see the AAV Plasmid Elements table . Figure 2: Plasmids required for rAAV vector production. Created... pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism...the central nervous system . Molecular Therapy, 10 (2), 302–317. https://doi.org/10.1016/j.ymthe.2004.05.024...the production of AAV vectors . Microorganisms, 12 (2), 384. https://doi.org/10.3390/microorganisms12020384...retrograde access to projection neurons . Neuron, 92 (2), 372–382. https://doi.org/10.1016/j.neuron.2016.09.021... contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need...wild-type serotype, and a very high infectivity across a broad range of cell types in vivo. AAV-DJ has...

  14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...divided by the off-target activity. Figure 2: Specificity is the measure of off-target activity...off-target activity, relative to eSpCas9 and SpCas9-HF across a number of different sites. Using a combined random...high degree of both specificity and sensitivity across a large number of loci and gRNA lengths. Recent...

  15. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...available in the adenovirus section below!   Fig. 2 - Viral vector components of AAV Adenovirus Adenoviruses...protocols! In reality, there are many shared components across viruses (AAV, lentivirus, etc.) and their functions... The components of these vectors are also split across several plasmids (often three), for safety purposes...

  16. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ... the Cas machinery where to cut.      Fig. 2: Heatmap indicating loci where SNPs reside on sgRNA...the breakdown of ancestry associated dependencies across ancestry groups (right).    Seeking an explanation...not represent the diversity of genetic variation across the human population. Rather, it primarily reflects...which SNPs map to thetargeting sequences of guides across 8 CRISPR libraries. Samples are divided into those...in CRISPR-based experiments with varying burden across ancestries. Sean A Misek, Aaron Fultineer, Jeremie...

  17. A Look at Addgene's QC Process

    Type
    Blog Post
    ...contain the expected plasmid features.  Figure 2. Addgene’s basic QC process. Created with BioRender.com... us to have a high standard of sequence quality across all plasmids in our collection. This standardization...we maintain a high standard of sequence accuracy across all of our plasmids and provide transparency in...sequence-verified constructs to share with scientists across the globe. This post was written by Alyssa Shepard...

  18. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... tabular HTML formatted output. Surprisingly only 2 tools provide FASTA output. A few notable tools allow...Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick D., et al. "DNA targeting specificity...determine the value of every sweet or toy strewn across the floor. In the final wake it all settles down...functional. It is also the only tool I have come across to offer classes of parameters based on the type...

  19. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...purification. Workflow Timeline Day 1: Purify Day 2: Buffer exchange and concentration Note: Both steps...7.4 1X PBS-MK buffer 100X Poloxamer 188 NaCl MgCl 2 KCl Centrifugal filter units (MWCO 100 kDa) Reagent...PBS-MK buffer Dissolve 5.84 g of NaCl, 26.3 mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final volume of...at 4 °C. 1X PBS-MK buffer Dissolve 26.3 mg of MgCl 2 , and 14.91 mg of KCl in 1× PBS in a final volume ...(C) (formulation buffer) Add 5 mL of Buffer B and 2 mL of 5 M NaCl to 43 mL PBS Procedure Preparation ...need more time, you can alternatively centrifuge for 2 h at 200,000 x g at 18 °C. Carefully take the QuickSeal...interface of the 60% and 40% gradient (see Figure 2) with an 18 ga needle. Place the first microcentrifuge...

  20. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine sensor In 2018, Lin Tian’s ...liquid, which causes the passive diffusion of DA across the semipermeable membrane from the extracellular...
Showing: 141 - 160 of 210 results