We narrowed to 312 results for: bon
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TypeBlog Post... engineered AAV are presently built on an AAV9 backbone and so will very likely cross-react with each ...
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Antibodies 101: Isotypes
TypeBlog Post...isotypes, such as the number and location of disulfide bonds, the number of constant domains, and the hinge region... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...-1a promoter. It’s a 3rd generation lentiviral backbone that must be used with lentiCas9-Blast or with... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein Tags ... -
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods
TypeBlog Post...Biology Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...lentivirus. Also, note that due to the size of the backbone, transformations require electroporation. References... -
Zhang Lab CRISPR Page
TypeCollection...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ... -
Antibodies 101: Conjugation
TypeBlog Post...cysteines, such as those that make up the disulfide bonds between antibody chains. 3) Endogenous carbohydrates... -
Antibodies 101: Affinity Reagents
TypeBlog Post...targets. Due to their size and lack of disulfide bonds, monobodies can be easily expressed in transfected... -
What's New in CRISPR - November 2021
TypeBlog Post...various delivery methods, including plasmid, ribonucleoprotein, and AAV. Read the paper in Nature Chemical... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...mcb.8.5.2159 Galvan A, Raper J, Hu X, Paré J, Bonaventura J, Richie CT, Michaelides M, Mueller SAL, Roseboom... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base editor... -
CRISPRainbow and Genome Visualization
TypeBlog Post...Plasmids Find Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...promoters were also tested in different plasmid backbones that varied in size or carried different genes... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...photoactivatable fluorescent proteins in empty backbones in the Michael Davidson Collection Find Photoswitchable... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post...Additional Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post...try to use an excess of insert compared to the backbone plasmid; a starting point could be a molar ratio... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...toolkit upon the Yeast Toolkit by replacing the backbone vector’s high-copy number bacterial ColE1 origin...