We narrowed to 200 results for: ckb

...components are provided in Golden Gate compatible backbones that can be combined with your gRNA and repair...

...digested vector backbone. This is accomplished by covalently connecting the sugar backbone of the two DNA..., the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into..., it is important to treat the digested vector backbone with a phosphatase before performing the ligation...

...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting...VP64) Set A Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing...

...plasmids for a given species are based on the same backbone to permit expression levels to be directly compared...

...what vector to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction...and purified your insert and recipient plasmid backbone bands away from the gel via your favorite gel ...have of uncut or self-ligating recipient plasmid backbone. Transformation Transform your ligation reaction...

...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ...

...., Chan, K. Y., Scherrer, J., Winchester, J., Blackburn, R. J., Campos, L. J., Man, K. N. M., Sun, J.,...

...Luciferase blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which you ... Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs Other...Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α ...pMKV060 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing firefly luc for monitoring plant transfection...pMKV057 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing WUS2 and IPT, with firefly luc for ...

...Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133...localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression ...localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...

...primers. These primers typically anneal to the backbone and can help you verify the ends of the insert...primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for reference...

...properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor λ Dex QY ...Amber (mVenus Y67C) FRET standards and cloning backbones Koushik et al. (2006) Cerulean, Venus, and VenusY67C...FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V) Goedhart et al. (...93% Positive and negative controls and cloning backbones for various FRET pairs with mTurquoise2 Mastop...mTurquoise2 Positive and negative controls and cloning backbones for red, yellow, and turquoise FRET pairs Gadella...

...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation...design for LIC is often as simple as using the backbone manufacturer's suggested leader sequence fused...

...fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable...Davidson lab has contributed the corresponding empty backbone with different fluorescent tags for you to create...images, tutorials, protocols, and more. Empty Backbones Plasmid Description Industry Return to top Blue...

...tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control...Browse the tables below to find popular empty backbones, expression vectors for transactivators, and vectors...inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber 60495 pSBtet-GP...Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled expression (On or Off) None...

...-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with the Voytas Golden...introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences...mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (...with the Voytas Lab Golden Gate TALEN kit. This backbone is suitable for the TALEN-based generation of ...

...for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations. Background...have of uncut or self-ligating recipient plasmid backbone. Transformation Proceed with the transformation...

...the Series drop down menu. Select the Trendline checkbox. Determine the linear equation and the R 2 of ... and choose Use Equation . Select the Show R 2 checkbox. Pro-Tip The R 2 of the trendline should be between...

...to top Recombinase-Dependent Empty Backbones Find an empty backbone to regulate your gene of interest....Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific... other recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase...

.../NLS Ple22 CCKBR pEMS1493 intron-lacZ/NLS Ple23 CCKBR pEMS1494 intron-lacZ/NLS Ple24 CCKBR pEMS1495 intron-lacZ...intron-lacZ/NLS Ple25 CCKBR pEMS1496 intron-lacZ/NLS Ple26 CCL27 pEMS1497 intron-lacZ/NLS Ple27 CCL27 ...

...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ligation of annealed...and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene...