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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 200 results for: ckb

Showing: 141 - 160 of 200 results

  2. Lentiviral Prep Service

    Type
    Collection
    ...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...genes and containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting...VP64) Set A Human CRISPR inhibition library in backbone pXPR_050 inhibiting 18,901 genes and containing...

  3. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...what vector to use, you can check out our Empty Backbone Reference . Background Subcloning by restriction...and purified your insert and recipient plasmid backbone bands away from the gel via your favorite gel ...have of uncut or self-ligating recipient plasmid backbone. Transformation Transform your ligation reaction...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...LacZ, plus Cre recombinase 60229 : sgRNA cloning backbone with Cre recombinase 60230 : sgRNA targeted to...adenocarcinoma pathology. These plasmids as well as a backbone plasmid for cloning new targets are described ...control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR This plasmid ...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes Zhang pSpCas9n...none S. pyogenes Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes...BsmBI none S. pyogenes Joung AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR 68347 Mammalian... cut S. pyogenes mCherry Kuhn AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI... SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian...AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian...yes, cut S. pyogenes Hyg Chen pUC57-Simple-gRNA backbone 51306 Xenopus BsaI none S. pyogenes Chen pMZ374...

  7. Plant Plasmids and Resources

    Type
    Collection
    ...top Empty Backbones The table below highlights plasmids that can be used as empty backbones for your gene...CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background...different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources...expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice. Includes a protocol (Link opens...includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase blog post . Empty Backbones Browse or search for popular empty backbone plasmids into which you ... Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs Other...Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α ...pMKV060 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing firefly luc for monitoring plant transfection...pMKV057 Firefly AtUbi10 BeYDV replicon on T-DNA backbone expressing WUS2 and IPT, with firefly luc for ...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone for bacterial expression under T7 control 101135...101135 pSNAP-tag (m) Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133...localization 101136 pCLIP-tag (m) Vector CLIP-tag Empty backbone for stable and transient mammalian expression ...localization 101127 pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag...pACP-tag (m)- 2 Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI...

  10. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...primers. These primers typically anneal to the backbone and can help you verify the ends of the insert...primers that are often found within the plasmid backbone (See Addgene's Sequencing Primers for reference...

  11. Ligation Independent Cloning

    Type
    Protocol
    ...Cloning You may also like... Cloning Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation...design for LIC is often as simple as using the backbone manufacturer's suggested leader sequence fused...

  12. Fluorescent Proteins: FRET

    Type
    Collection
    ...properties. Where available, we have listed cloning backbones and control plasmids. Donor Acceptor λ Dex QY ...Amber (mVenus Y67C) FRET standards and cloning backbones Koushik et al. (2006) Cerulean, Venus, and VenusY67C...FRET Positive and negative controls and cloning backbones for mTurquoise2-mVenus(L68V) Goedhart et al. (...93% Positive and negative controls and cloning backbones for various FRET pairs with mTurquoise2 Mastop...mTurquoise2 Positive and negative controls and cloning backbones for red, yellow, and turquoise FRET pairs Gadella...

  13. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab....Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable...Davidson lab has contributed the corresponding empty backbone with different fluorescent tags for you to create...tutorials, microscopy protocols, and more. Empty Backbones Plasmid Description Industry Return to top Blue...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...tet-inducible vectors. Tetracycline-inducible Backbones Find backbones or constructs to use for cloning to control...Browse the tables below to find popular empty backbones, expression vectors for transactivators, and vectors...inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-Advanced TRE3G Johannes Zuber 60495 pSBtet-GP...Tight TRE David Sabatini 19407 pTREtight2 Empty backbone for Tet-controlled expression (On or Off) None...

  15. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations. Background...have of uncut or self-ligating recipient plasmid backbone. Transformation Proceed with the transformation...

  16. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...the Series drop down menu. Select the Trendline checkbox. Determine the linear equation and the R 2 of ... and choose Use Equation . Select the Show R 2 checkbox. Pro-Tip The R 2 of the trendline should be between...

  17. TALEN Plasmids and Kits

    Type
    Collection
    ...-DD and pCS2TALE3-RR are next generation TALEN backbone vectors designed for use with the Voytas Golden...introduced to the N- and C-terminus of the pTAL3 TALEN backbone, which were initially published by Sangamo BioSciences...mammalian cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (...with the Voytas Lab Golden Gate TALEN kit. This backbone is suitable for the TALEN-based generation of ...

  18. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...to top Recombinase-Dependent Empty Backbones Find an empty backbone to regulate your gene of interest....Recombinase Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific... other recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase...

  19. The Pleiades Promoter Project

    Type
    Collection
    .../NLS Ple22 CCKBR pEMS1493 intron-lacZ/NLS Ple23 CCKBR pEMS1494 intron-lacZ/NLS Ple24 CCKBR pEMS1495 intron-lacZ...intron-lacZ/NLS Ple25 CCKBR pEMS1496 intron-lacZ/NLS Ple26 CCL27 pEMS1497 intron-lacZ/NLS Ple27 CCL27 ...

  20. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...The plasmids listed here are provided as empty backbones that utilize Golden Gate cloning to insert the...desired guide RNA sequence by digestion of the backbone with BbsI followed by a quick ligation of annealed...and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene...
Showing: 141 - 160 of 200 results