...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...Easi-CRISPR works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...

...binding non-specifically – try optimizing your blocking and washing conditions or consider using a different...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order...

...searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...the actuator’s subcellular localization using trafficking signals and named it Magneto 2.0. Using Magneto2.0...

...found here. Figure 3: A surprise for the author. Checking Cas9 expression Overview: By Western blotting ...C, Vogelstein B, Bunz F. Genetic knockouts and knockins in human somatic cells. Nature Protocols 2007;...

...silencing effect through targeted mRNA degradation, blocking translation, or chromatin modifications of complementary...researchers studying plants and other organisms lacking this innate immunity need not worry and have benefited...

...pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research include...collaborative therapies must be explored. When attacking cancer, multiple immunotherapy targets can be ...

...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...

...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...

...using the application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries...thermodynamic and experimental parameters (neighbor stacking energies, loop entropy effects, cation concentrations...

...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...

...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...

...of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible guest posts...

...Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman, Unearthed Comics Feeling...

...keep track of reagents in your lab. With barcode tracking, you will always be able to locate materials quickly...

...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

...If you’re new to plasmids, we highly recommend checking out the video). Tom has made many more Science...

...powered by 100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown...

...Biotechnology at Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata...