We narrowed to 334 results for: cki

...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition, inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015)...

...Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein...Cas9 nickase to improve target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety...

...binding non-specifically – try optimizing your blocking and washing conditions or consider using a different...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order...

...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...Easi-CRISPR works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...

...searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...the actuator’s subcellular localization using trafficking signals and named it Magneto 2.0. Using Magneto2.0...

...silencing effect through targeted mRNA degradation, blocking translation, or chromatin modifications of complementary...researchers studying plants and other organisms lacking this innate immunity need not worry and have benefited...

...found here. Figure 3: A surprise for the author. Checking Cas9 expression Overview: By Western blotting ...C, Vogelstein B, Bunz F. Genetic knockouts and knockins in human somatic cells. Nature Protocols 2007;...

...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...

...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...

...pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research include...collaborative therapies must be explored. When attacking cancer, multiple immunotherapy targets can be ...

...using the application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries...thermodynamic and experimental parameters (neighbor stacking energies, loop entropy effects, cation concentrations...

... number of E. coli strains below and recommend checking out Addgene’s blog posts about common E. coli ...and their recommended concentrations. We suggest checking your plasmid's datasheet or the plasmid map to... to the sequence of the complementary strand. Mimicking the DNA replication process requires the four ...

...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...

...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

...of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible guest posts...

...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...

...Zimmerman   5)   “Beatrice the Biologist” by Katie McKissick       Sara Zimmerman, Unearthed Comics Feeling...

...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

...keep track of reagents in your lab. With barcode tracking, you will always be able to locate materials quickly...