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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 333 results for: cki

Showing: 141 - 160 of 333 results

  1. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...we developed a great plan to start consistently knocking out high-quality videos for you all. The next ... me, and to move it out of the way when it was blocking some more important lab work from being done. ...

  2. Evolution of Lab Techniques

    Type
    Blog Post
    ...nucleotides, called dideoxynucleotides (ddNTPs), lacking the 3’ hydroxyl group required for the extension...mouse lines, such as BAC transgenic or traditional knockins/knockouts, a thing of the past. In the sequencing...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein...Cas9 nickase to improve target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety...

  4. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...binding non-specifically – try optimizing your blocking and washing conditions or consider using a different...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order...

  5. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...Easi-CRISPR works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...the actuator’s subcellular localization using trafficking signals and named it Magneto 2.0. Using Magneto2.0...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...

  10. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research include...collaborative therapies must be explored. When attacking cancer, multiple immunotherapy targets can be ...

  12. CRISPR Guide

    Type
    Guide
    ...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition; inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall, E. M., & Myers, R. M. (2015)...

  13. Deep Dive: qPCR

    Type
    Blog Post
    ...using the application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries...thermodynamic and experimental parameters (neighbor stacking energies, loop entropy effects, cation concentrations...

  14. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    ...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...

  18. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    ...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...
Showing: 141 - 160 of 333 results