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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 372 results for: construct

Showing: 141 - 160 of 372 results

  1. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...the plasmid name: This is the name given to the construct by the depositing lab. There may be other plasmids... Principal Investigator (PI) in whose lab the construct was made. The depositor name links to a page listing...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription Reporter:...at Addgene. Cell marking/selection: Expression constructs like plasmids often include GFP as a marker to...

  3. Plant Plasmids and Resources

    Type
    Collection
    ...vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes Stuttmann...containing several cassettes and generate multi-construct transgenic plants - Jan Lohmann. MoClo Plant Parts... for hierarchical assembly of plant multigene constructs - Johannes Stuttmann. MoChlo: Modular Cloning...

  4. TALEN Guide

    Type
    Collection
    ...small, repeat-laden DNA regions into a single construct would be technically challenging. Thanks to efforts... of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian...6051):1843-6. Pubmed PMID: 21960622 . Efficient construction of sequence-specific TAL effectors for modulating...

  5. Chemogenetics Plasmids

    Type
    Collection
    ... our inventory of chemogenetic constructs, including DREADD constructs....

  6. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...stranded break constructs. Plasmids 171098 and 175570 allow conversion to a dual guide construct. Plasmids ...

  7. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...experiments. You may have to design several different constructs to find one that could be used for the purpose...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular ...

  8. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...assay, and easy to purify using histidine-tagged constructs and routine protein and molecular biology techniques... window) Acknowledgments The MCC and plasmid construction was funded by NSF IUSE 1726932 and NSF RCN-UBE...

  11. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...labs how they can benefit from depositing their constructs to the repository, and getting feedback from ...

  12. TALEN Plasmids and Kits

    Type
    Collection
    ...documentation allow one to efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit...Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination constructs were designed for optimal mammalian expression...Gate TALEN 2.0 1000000030 Yamamoto Lab TALEN Construction and Evaluation Accessory Pack Takashi Yamamoto...

  13. What's New in CRISPR - May 2019

    Type
    Blog Post
    ...Molecular Cell Find the Nme2Cas9 All-in-One AAV constructs CRISPR interference in Candida albicans A new...

  16. CRISPR Plasmids - Tagging

    Type
    Collection
    ... locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe harbor" locus...pathway, instead of HDR, which makes it easy to construct a donor vector, because MMEJ utilizes very short...method to tag endogenous proteins by using donor constructs that harbor synthetic short homology arms. Notably...

  17. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ... of different studies. Elly Tanaka’s lab has constructed several vectors for use in the axolotl including...GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in Arabidopsis....

  18. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...two-photon illumination than previous GEVIs. Some constructs are also available as viral vectors (here and...and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-...

  19. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...cell types, while shRNA and siRNA are synthetic constructs introduced through external methods. shRNA processing...C. (2006). Design and cloning strategies for constructing shRNA expression vectors. BMC Biotechnology,...
Showing: 141 - 160 of 372 results