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We narrowed to 306 results for: construct

Showing: 141 - 160 of 306 results
  1. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ...Promega Plasmid Collection that express similar constructs available at Addgene. Read the original paper...et al., 2025). They designed HiBiT-tagged TP53 constructs, one with an R175H mutation to match a frequent...
  2. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ...mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology...template, you'd use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene...
  3. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...hypothesis that the lower expression levels of SunTag constructs would avoid negative effects on cell physiology...
  4. AAV Viral Preps

    Type
    Collection
    ...Addgene's viral vectory packaging service. Browse constructs currently available in viral format....
  5. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...the research directions of the lab, gives you constructive feedback on your research, and teaches you how...cover letters, fellowships, etc. Second, get constructive feedback from experts. If someone crosses out...
  6. TALEN Engineering

    Type
    Collection
    ...transcriptional activator target sites and to simplify the construction of TALEN expression plasmids using REAL, REAL-Fast...
  7. Lentivirus Plasmids

    Type
    Collection
    ...human MSCs. Use with second-generation packaging construct encoding tat . Jakob Reiser 35617 pCAG-Eco Ecotropic...strain). Use with second-generation packaging construct encoding tat . Jakob Reiser 35616 pCAG-VSVG VSV-G-expressing...
  8. Modular Cloning Guide

    Type
    Guide
    ... for assembly of CRISPR/Cas9 constructs for use in plants. The constructs are made with zCas9i, a high...a growing number of libraries containing pre-constructed standardized modules. In the first assembly step...vectors permits the creation of even more complex constructs constrained only by the ability of E. coli to...standardized parts to create eukaryotic multigene constructs. MoClo Plant Parts Kit Plant Expression Nicola... for hierarchical assembly of plant multigene constructs. MoClo Plant Parts III: Transformation & Genome...containing several cassettes and generate multi-construct transgenic plants. GreenGate 2.0 Toolkit Plant... (Lohmann) to create plasmids with multi-gene constructs for plant transformation. GoldenBraid 2.0 Kit...
Showing: 141 - 160 of 306 results