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We narrowed to 494 results for: control

Showing: 141 - 160 of 494 results

  1. Working with Nuclear Receptors

    Type
    Blog Post
    ...reproduction. Both physiological processes are controlled by deliberate and compulsory actions instigated...complex can serve as a rate-limiting conduit to control the actions of the whole complex.  For example,...N. J.and O'Malley, B. W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators...

  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...competent cells, don’t forget to do a negative control (plate your cells without adding plasmid) to make...have contamination.  Also conduct a positive control (test a plasmid that transforms well in other competent...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... under the control of the cI857 temperature-sensitive repressor. The temperature-controlled expression... at a dose and duration sufficient to kill all control cells and surviving “mutant” colonies were deep...GTPases involved in cell signaling pathways that control many cellular processes such as differentiation...gRNA backbone plasmids with Addgene. Additional control plasmids for the TKO library will be available ...luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. The system...Furthermore, dCAS9-VPR expression is under the control of the TRE promoter, which allows for doxycycline-inducible...result is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...to hook up your lasers and not have the proper controls or a proper behavioral readout that allows you...not be an appropriate test.  Of course, proper controls are required and include, for example, confirmation...Carolina) that offer his constructs. Importantly, control viruses (not expressing the opsin) are also available...into the correct direction and expressed (under control of a generic promoter). A DIO opsin allows cell...the fluorescent proteins are used as a critical control. Depending on the AAV serotype, promoter of choice...

  5. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...wild-type copies of MYBPC3 (versus 47.4% of untreated controls.) 27.6% of embryos carried indels at the mutation...blastocyst stage at similar rates to untreated controls. Ma et al. performed sequencing of 23 potential... conducted whole genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all cases,...

  6. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...native regulatory elements. Controls for RaPID experiments RaPID negative controls include both scrambled ...enrichment) when compared to a scrambled RNA motif control). BioID2 had no detectable signal at the same time...

  7. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ... our members took an Arduino microcontroller and a motor controller and made it spin again!  We’ve also...instance, the centrifuge in the picture had a broken controller and would not spin. It was going to cost far ...far more than it was worth to repair the controller and a new replacement was not possible, but one of our...

  8. Five Popular Model Organisms

    Type
    Blog Post
    ...easily control the level and spatiotemporal expression of a gene of interest. This type of control over ... modular vectors that can be used to optimally control gene expression in specific cells of the fly (Pfeiffer...using HOTcre, a method that uses heat induction to control the temporal expression of different transgenes...

  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... A fast blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases...recombinases to manipulate genomes, scientists can control expression of their genes of interest. While recombinases...their activity can be fine-tuned and precisely controlled through the use of optogenetics. Optogenetic ...

  10. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...structure. It is thought that poly(A)s are used to control the cellular concentration of regulatory RNAs and... and may additionally act as a quality control mechanism to rid the cell of mis-folded RNAs. Eukaryotic...Resources on the Addgene Blog Learn how the Promoter Controls Gene Expression Learn about New Cloning Techniques...

  11. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...drug-resistant population compared to a non-treated control group. In this scenario, gRNAs that are “enriched...As with any experiment, the use of appropriate controls, multiple replicates and several cell types can...Titering Your Lentivirus Plasmids 101: NGS Quality Control for Pooled Libraries Find More CRISPR Resources...future therapeutic targets for diseases causing uncontrolled cell growth, such as cancer. Figure 2...

  13. Deisseroth INTRSECT Collection

    Type
    Collection
    ...recombinase recognition sequences enables directional control of the portion of the ORF that is sandwiched between... INTRSECT recombinases, viruses, and important controls to consider as part of experimental design. How-to..., Paninski L, Li B. 2017. The central amygdala controls learning in the lateral amygdala. Nat Neurosci...Meletis K. 2019. A hypothalamus-habenula circuit controls aversion. Mol. Psychiatry 24(9):1351-1368. PubMed...

  14. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...an effort to obtain even more precise temporal control of gene knockout or activation, the c-terminal ...). This system provides users greater temporal control over CRISPR/Cas9 mediated genome modification and...Optogenetics is a powerful tool that utilizes light to control and monitor individual living cells in order to... allows scientists to spatially and temporally control which genes are turned on or off in a given area...strategy is that each "bit" of information must be controlled independently; that is, the signal to record ...Photoswitchable tools for spatial and temporal control of cell events Three years ago, Brian Kuhlman’s...localization in mammalian cell culture and reversible control of signalling pathways. These LIDs are generalizable...

  15. Antibodies 101: Validation

    Type
    Blog Post
    ...you see a similar antibody signal between your control and your knock-out sample, you should be concerned...antibody binding in the transfected cells but not in control cells. But (there is always a ‘but’), you should...encoding this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run...well as a third unrelated antibody as a negative control (A). The two antibodies to your protein of interest...

  16. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...unilateralis is a fungus that infects ants and controls their behavior. These ants become “zombies” and...display imitating a moving caterpillar. The worm controls the snail’s behavior, forcing it out of hiding...

  17. Worm Expression Resources

    Type
    Collection
    ...silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. - ...Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...

  18. mTOR Pathway

    Type
    Collection
    ...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012...in cancer promotes the conditions needed for uncontrolled cell growth and proliferation, including an ...

  19. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...advantages degron tags can offer over other protein control methods and compare the commonly available types...etc. Firstly, they are reversible mechanisms of control. Protein degradation can be induced by the addition... Tsien RY, Lin MZ. Tunable and reversible drug control of protein production via a self-excising degron...

  20. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...2007). Thus, RABV transsynaptic spread can be controlled by selectively supplying the G protein in trans...optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal populations. In... Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression with FLEx Vectors Resources on...
Showing: 141 - 160 of 494 results