We narrowed to 495 results for: control

...of using loxP sites is the inability to tightly control which loxP sites recombine if more than two are...allowing for two separate recombination events to be controlled in parallel. Have you used the Cre-lox system...PubMed Central PMCID: PMC49604. 4. Independent control of immunoglobulin switch recombination at individual...

...reproduction. Both physiological processes are controlled by deliberate and compulsory actions instigated...complex can serve as a rate-limiting conduit to control the actions of the whole complex.  For example,...N. J.and O'Malley, B. W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators...

...target sequence lower the repression level. By controlling the number of mismatches we can obtain a range...reporters, mCherry and sfgfp in this case, can be controlled using a plasmid‐borne CRISPR array coding for...

...competent cells, don’t forget to do a negative control (plate your cells without adding plasmid) to make...have contamination.  Also conduct a positive control (test a plasmid that transforms well in other competent...

... under the control of the cI857 temperature-sensitive repressor. The temperature-controlled expression... at a dose and duration sufficient to kill all control cells and surviving “mutant” colonies were deep...GTPases involved in cell signaling pathways that control many cellular processes such as differentiation...gRNA backbone plasmids with Addgene. Additional control plasmids for the TKO library will be available ...luciferase activity in the Co-IP compared of a control Co-IP indicates stronger interaction. The system...Furthermore, dCAS9-VPR expression is under the control of the TRE promoter, which allows for doxycycline-inducible...result is genomic integration of dCas9-VPR and controlled expression of the dCas9-VPR transgene by doxycycline...

...to hook up your lasers and not have the proper controls or a proper behavioral readout that allows you...not be an appropriate test.  Of course, proper controls are required and include, for example, confirmation...Carolina) that offer his constructs. Importantly, control viruses (not expressing the opsin) are also available...into the correct direction and expressed (under control of a generic promoter). A DIO opsin allows cell...the fluorescent proteins are used as a critical control. Depending on the AAV serotype, promoter of choice...

...wild-type copies of MYBPC3 (versus 47.4% of untreated controls.) 27.6% of embryos carried indels at the mutation...blastocyst stage at similar rates to untreated controls. Ma et al. performed sequencing of 23 potential... conducted whole genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all cases,...

...native regulatory elements. Controls for RaPID experiments RaPID negative controls include both scrambled ...enrichment) when compared to a scrambled RNA motif control). BioID2 had no detectable signal at the same time...

... our members took an Arduino microcontroller and a motor controller and made it spin again!  We’ve also...instance, the centrifuge in the picture had a broken controller and would not spin. It was going to cost far ...far more than it was worth to repair the controller and a new replacement was not possible, but one of our...

...easily control the level and spatiotemporal expression of a gene of interest. This type of control over ... modular vectors that can be used to optimally control gene expression in specific cells of the fly (Pfeiffer...using HOTcre, a method that uses heat induction to control the temporal expression of different transgenes...

... A fast blue-light inducible Cre for precise control of bacterial gene expression By Meghan Rego Recombinases...recombinases to manipulate genomes, scientists can control expression of their genes of interest. While recombinases...their activity can be fine-tuned and precisely controlled through the use of optogenetics. Optogenetic ...

...structure. It is thought that poly(A)s are used to control the cellular concentration of regulatory RNAs and... and may additionally act as a quality control mechanism to rid the cell of mis-folded RNAs. Eukaryotic...Resources on the Addgene Blog Learn how the Promoter Controls Gene Expression Learn about New Cloning Techniques...

...drug-resistant population compared to a non-treated control group. In this scenario, gRNAs that are “enriched...As with any experiment, the use of appropriate controls, multiple replicates and several cell types can...Titering Your Lentivirus Plasmids 101: NGS Quality Control for Pooled Libraries Find More CRISPR Resources...future therapeutic targets for diseases causing uncontrolled cell growth, such as cancer. Figure 2...

...success. Sometimes, you might have a few positive control genes on which to test the validity of your biological... the whole point is that there are no positive controls for this phenotype – you’ve developed a new model...

...an effort to obtain even more precise temporal control of gene knockout or activation, the c-terminal ...). This system provides users greater temporal control over CRISPR/Cas9 mediated genome modification and...Optogenetics is a powerful tool that utilizes light to control and monitor individual living cells in order to... allows scientists to spatially and temporally control which genes are turned on or off in a given area...strategy is that each "bit" of information must be controlled independently; that is, the signal to record ...Photoswitchable tools for spatial and temporal control of cell events Three years ago, Brian Kuhlman’s...localization in mammalian cell culture and reversible control of signalling pathways. These LIDs are generalizable...

...you see a similar antibody signal between your control and your knock-out sample, you should be concerned...antibody binding in the transfected cells but not in control cells. But (there is always a ‘but’), you should...encoding this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run...well as a third unrelated antibody as a negative control (A). The two antibodies to your protein of interest...

...unilateralis is a fungus that infects ants and controls their behavior. These ants become “zombies” and...display imitating a moving caterpillar. The worm controls the snail’s behavior, forcing it out of hiding...

...2007). Thus, RABV transsynaptic spread can be controlled by selectively supplying the G protein in trans...optogenetic or chemogenetic receptors) enables complex control of specifically defined neuronal populations. In... Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression with FLEx Vectors Resources on...

...live cells.  Controls  When designing experiments, it’s important to include proper controls to assess the... p53 tetramerization in response to DNA damage controls the p53 response. Gaglia et al. Mol Syst Biol....

...donor templates and sequencing primers for quality control. At such a scale, automation is crucial, both for... to avoid re-cutting when needed. It gives you control over your data. CrispyCrunch keeps a record of ...repair outcomes, which is important for quality control. The only ones we found that worked with NGS data...