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We narrowed to 200 results for: crispr cas9 grna plasmid

Showing: 141 - 160 of 200 results

  1. Hot Plasmids: Fall 2024

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    Blog Post
    ... Overview of the dual-plasmid CRISPR/Cas9 workflow in M. abscessus. A) Sth1Cas9 mediates double strand... in Mycobacterium abscessus using a dual-plasmid CRISPR/Cas9 system By Aryana Khosravani Deborah Hung’...Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system for gene disruption in Mycobacterium abscessus...first use of CRISPR/Cas9 in M. abscessus. The simplicity and efficiency of this dual-plasmid approach makes...Clatworthy A, and Hung, D. (2024). A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic...selection. Using an inducible Cas9 expression and removing the sgRNA plasmid after identifying successful...curing the mCherry-sgRNA plasmid leaves an edited strain carrying Cas9 to allow the introduction of additional...

  2. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post
    ...Addgene.org Find CRISPR Plasmids for Your Research Check out Our CRISPR Guide Find Validated gRNAs ...Addgene Blog Catch Up on Your CRISPR Background with CRISPR 101 Learn about CRISPR Delivery Methods Validate...sites 2. Design a CRISPR screen to target and disrupt the NANOG enhancers 3. CRISPR out the enhancers ...design a CRISPR screen to disrupt these enhancers. The third and final step is to conduct our CRISPR screen...RNAs (gRNAs) across the population. Recall that guide RNAs are the targeting mechanism for CRISPR. Therefore...embryonic stem cells, and conclude with a targeted CRISPR screen that can disrupt these enhancers. A real-world...have more to say about clinical applications of CRISPR in the next section. In order to denote the NANOG...

  3. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand... made comparisons between CRISPR components expressed from different plasmid backbones. They noted that...comparisons of CRISPR parts. The Fragmid system was used to assess how different lentiviral plasmid backbone...targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing of sgRNAs against unique... the most intuitive CRISPR MoClo application is to assemble multiple unique sgRNAs into a multi-TU targeting...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...

  4. CRISPR 101: RNA Editing with Cas13

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    Blog Post
    ...Resources on Addgene.org Browse All CRISPR Plasmids Check out our CRISPR Guide Pages ...editing with CRISPR-Cas13.” Science (2017):pii: eaaq0180. PMID: 29070703. Find plasmids from this paper...targeting with CRISPR-Cas13.” Nature 550(7675) (2017):280-284. PMID: 28976959. Find plasmids from this paper... VI-A CRISPR-Cas Enzymes." Mol Cell. 66(3) (2017):373-83. PubMed PMID: 28475872. Find plasmids from this... out our CRISPR Featured Topic Page Learn about RNA Targeting with Cas13a Learn about CRISPR DNA Base ...enzymes are quickly becoming major players in the CRISPR field. Just a year after Feng Zhang’s lab identified...C2c2) (Abudayyeh et al., 2016) as a RNA-targeting CRISPR enzyme, they adapted Cas13b for precise RNA editing...

  5. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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    Blog Post
    ...Silvana Konermann, the first generation of CRISPR-Cas9 plasmids were not designed with gene activation in... the CRISPR system was a large one.  The question for her then was this: Can you engineer CRISPR-Cas9 ...describe just such a system, which they call CRISPR/Cas9 Synergistic Activation Mediator or SAM. SAM is...Two-way CRISPR screening In October, Jonathan Weissman’s lab introduced additional pooled CRISPR libraries... transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE,...components: 1. A nucleolytically inactive Cas9-VP64 fusion; 2. A sgRNA incorporating two MS2 RNA aptamers at...CRISPR technology has changed how scientists edit and control genes, but according to the Broad Institute's...

  6. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...fully functional Cas9 upon dimerization. Splitting wild-type Cas9 into n-terminal (Cas9(N)-2xNLS) and c-terminal... control over CRISPR/Cas9 mediated genome modification and gene expression. The plasmids associated with...Addgene Blog Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts ...Rapamycin-inducible Cas9 sets (Addgene plasmids 62883 &62884; 62885 & 62886) Rapamycin-inducible dCas9-VP64 activator...called CRISPR-Display, that may help answer this difficult question. CRISPR-Display uses the dCas9 enzyme...DNA loci. In CRISPR-Display, your RNA of interest is fused to an sgRNA used to direct Cas9 to a specific...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...

  7. Hot Plasmids and Viral Preps - July 2021

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources ...acssynbio.0c00192   The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added to...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New... lentiviral CRISPR library from Alec Kimmelman targets mouse metabolic genes with ~6 gRNAs per gene.  ...genome-wide CRISPR knockout library from Xiaole Shirley Liu's lab targets all mouse genes with 10 gRNAs per gene...

  8. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

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    Blog Post
    ....org Read the CRISPR guide Find CRISPR plasmids by function Browse our entire plasmid collection ...Guide RNAs Visit our CRISPR topic page Learn the basics of CRISPR Download the CRISPR 101 eBook Resources...prime editor consists of a Cas9 nickase fused to a reverse transcriptase. The Cas9 nickase is guided to the... that most Cas9-induced DSBs are repaired by non-homologous end joining. As a result, Cas9 treatment causes... ~ ~ ✓ ✓ ✓ R221K N394K Cas9 mutations Improve Cas9 nuclease activity ~ ~ ~ ~ ...this evolving field! Find prime editing plasmids! Find plasmids from David Liu's lab! This post was originally...degraded, the truncated pegRNA can still guide Cas9 and compete with full pegRNAs for access to the target...

  9. CRISPR Plasmids for Genomic Visualization

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR Visualize CRISPR Plasmids:...CRISPR/Cas plasmids for labeling and visualization of genomic loci. CRISPR...Plasmids: Visualize Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...

  10. CRISPR Plasmids - Purify Genomic Loci

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...search the tables below for CRISPR plasmids to help purify genomic loci. Plasmids are available for expression...interest in vivo. For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus...Catalytically inactive CRISPR plasmids for purification of target loci. CRISPR... CRISPR Purify CRISPR: Purify Genomic ...Genomic Loci Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  11. Hot Plasmids: Spring 2025

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    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. ...Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set for expressing tagged proteins...and AAV plasmid sets launching later this year!  Find Addgene's own Lentiviral backbone plasmids here! ...RNA-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon system with the...scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology... synaptic imaging applications. Find iGluSnFR4 plasmids here! Aggarwal, A., Negrean, A., Chen, Y., Iyer...powerful tools in a scientist's toolkit is the empty plasmid backbone: the DNA vector that contains everything...

  12. CRISPR Plasmids - Parasites

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR expression plasmids that have been designed for use in parasites. CRISPR... CRISPR Parasites CRISPR Plasmids...Plasmids: Parasites Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...research for decades. Recent developments with CRISPR-Cas9 gene editing have propelled both reverse and ...than NHEJ. ID Plasmid Gene/Insert Selectable Marker PI Publication Libraries Toxoplasma CRISPR Knockout Pooled...

  13. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post
    ...other CRISPR reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide... genome, but they’re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks. ...single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time, ...converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et al...efficiency of plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and... A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular Therapy - ...and enrichment of DNA editing by APOBEC–Cas9 or cleavage by Cas9 in living cells. Nucleic Acids Research...

  14. CRISPR Plasmids - RNA Targeting

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    ...CRISPR RNA Targeting CRISPR Plasmids: RNA Targeting Browse CRISPR Plasmids By Function Genome Editing Cut ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...or search the tables below for CRISPR RNA targeting plasmids. Plasmids are available for expression in...CRISPR plasmids for targeting RNA sequences, which include the enzymes C2c2/Cas13a and Cas13b. CRISPR...Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Bacterial ID Plasmid Gene/Insert...Publication Plant ID Plasmid Gene/Insert PI Publication Do you have suggestions for other plasmids that should ...

  15. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post
    ...using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need... recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast ... (ARG) plasmids to better monitor gene expression in vivo using ultrasound. These ARG plasmids contain... custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for in vitro...Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage...

  16. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

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    Blog Post
    ... on the Addgene Blog CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 RNA Interference in Plant... Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids and Resources ...researcher. Among the most well-known CRISPR-associated nucleases are Cas9 and Cas12a, the heavyweights in ...like Cas9. This is where TnpB offers a clear advantage. At approximately one-third the size of Cas9 or ...Research Institute. When it comes to genome editing, CRISPR is a name that resonates with nearly every biologist...amino acids in size. To give a visual analogy: if Cas9 or Cas12a is a soccer ball, TnpB is a baseball —...ancestors of Cas12 proteins. Functionally, while Cas9 contains two nuclease domains (HNH and RuvC-like...

  17. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post
    ....org Read our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...hyperthermostable Cas9 CRISPR nuclease that is active up to 100°C.  The IgnaviCas9 protein is 1,241 amino acids...proteins Read all of our CRISPR blog posts Read about GeoCas9, another thermostable Cas9 Resources on Addgene.org...indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is a type II-C Cas9. IgnaviCas9 is most similar to...thermostable Cas9 enzymes have previously been characterized: GeoCas9 from the Doudna lab and ThermoCas9 from ...Find IgnaviCas9 here! What can you do with IgnaviCas9? Why would a thermostable Cas9 be useful? It can enable...launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year it was wonderful to learn...

  18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    Blog Post
    ...every isolated plasmid sample and normalize each to fall within a 10 fold range. Plasmids that don't meet...A broader view of the whole plasmid To confirm the sequence of the plasmid, we examine three things: ...expected and do not affect plasmid function, we will update information on the plasmid’s page. Sometimes we’re...’re missing a piece of the plasmid There are regions of some plasmids that are particularly difficult ...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are ...bottom of the plasmid page. Viral preps and pooled libraries In addition to regular plasmid sequencing,...sequence all plasmids in the pool but instead selectively sequence the variable portion of the plasmid in the...

  19. Validated gRNA Sequences

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    Collection
    ...like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences...Addgene's CRISPR Plasmids and Resources page have been used to indicate the Cas9 application the gRNA was designed... Genome Engineering CRISPR Validated gRNA Sequence Datatable Validated gRNA Target...PAM sequence). Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...Validated gRNA Sequence Datatable Target Gene Target Species Target Sequence Plasmid ID Application Cas9 Species...validated for use in CRISPR experiments. This datatable is compiled from plasmids in Addgene's database...Does a given gRNA sequence exactly match your genomic target? Variation between a given gRNA sequence and...

  20. Zhang Lab CRISPR Page

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    ...PubMed . Return to AAV In vivo plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing...CRISPR Cas9 gRNA plasmids from the Zhang lab Genome...Return to top Cas9 SAM transcriptional activation plasmids and screening library CRISPR/Cas9 Synergistic...backbone cloning information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors ...CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo Delivery... 24. PubMed . Return to SpCas9 plasmids GeCKO Library Genome-scale CRISPR-Cas9 knockout screening in human... Genome Engineering CRISPR Zhang Lab CRISPR Plasmids Zhang...
Showing: 141 - 160 of 200 results