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We narrowed to 274 results for: crispr cas9 plasmids

Showing: 141 - 160 of 274 results

  1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    Blog Post
    ...development in immunotherapy is the combination of the CRISPR/Cas9 methodology with T cell receptor engineering ...to using randomly integrating viral vectors, CRISPR/Cas9 genome editing allows targeted engineering of... al. "Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection." Nature 543.7643 (...Resources on Addgene.org Browse Cancer Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the immune system and ...analyse the tumor microenvironment (one of the plasmids on her poster is pTomo-Ras which has been deposited... immunology resources. If you have a great new plasmid you’d like to share with the scientific community...

  2. PiggyBac-ing Through the Genome Editing Field

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    Blog Post
    ...the ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source... the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges...piggyBac™ transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations ...donor plasmid carrying the DNA edit of interest, along with a site-specific nuclease (such as CRISPR/Cas9...technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge...piggyBac™ Transposon Catch up on Everything CRISPR with Our CRISPR Guide Deposit Your piggyBac™ Vectors! ...useful resource. While Addgene cannot distribute plasmids with the piggyBac™ transposase itself, please ...

  3. New CRISPR Tools: Cas7-11 and PASTE

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    Blog Post
    ... and Flexibility to CRISPR Engineering CRISPR 101: RNA Editing With Cas13  CRISPR Cheat Sheet Resources...RNA or DNA via a CRISPR mechanism. The weird black pellet's DNA yielded a new CRISPR effector protein ...has dual activities: processing pre-CRISPR RNA into mature CRISPR RNA and cleaving RNA at positions defined...DNA with CRISPR-driven precision into a genome, can be considered the fourth generation of CRISPR, one which...cargo via Cas9-directed integrases.   Use PASTE can be delivered via a single dose of plasmids, works... part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular...been working on two tools that expand and exploit CRISPR, both of which we're featuring in today's blog....

  4. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...Addgene.org Visit our CRISPR guide Find CRISPR plasmids for your research Browse plasmids for base editing ... the ZFNs.   Genetic Engineering Era: Post CRISPR  CRISPR The strategy of using a protein to recognize... Find more blog posts about CRISPR Learn more about the history of CRISPR Read about other genome editing... The new frontier of genome engineering with CRISPR-Cas9. Science 346:1258096 . https://doi.org/10.1126... Zhang F (2013) Genome engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https:...during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized and understood the DNA, the ...breakthrough in the world of genetic engineering as CRISPR-Cas editing soon replaced the protein-based DNA-binding...

  5. CRISPR 101: Drug discovery and CRISPR

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    Blog Post
    ...on the Addgene blog CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to Edit Disease Out of...first time CRISPR User (by a first time CRISPR User) Additional resources on Addgene.org CRISPR Guide ... CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...human trial phases.   So how does CRISPR factor into this workflow? CRISPR both speeds up the process and...potential targets by CRISPR can accelerate the drug pipeline. High-throughput CRISPR screens can be used...where CRISPR can save time and money to make sure quality candidates are selected.      CRISPR aids... discovery pipeline.   CRISPR and drugs in the future While CRISPR has already done a lot for drug...

  6. Human Germline Editing Using CRISPR

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    Blog Post
    ...Once with CRISPR Resources on Addgene.org Browse All CRISPR Plasmids Check Out Our CRISPR Guide Pages... and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs, and electroporated...whole genome sequencing in selected control and CRISPR-Cas9 injected embryos. In all cases, they did not ...Addgene Blog Check out Our New CRISPR Topic Page Read about High Fidelity CRISPR Variants Target Multiple Genes... updated in response to this paper. Any hint of CRISPR editing in human embryos has been met with a storm...talk about, as it represents another step towards CRISPR germline editing of disease-causing mutations. ...mosaicism. To circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus...

  7. Addgene’s Top 10 Blog Posts from 2014

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    Blog Post
    ...Patrick CRISPR-Cas9 FAQs Answered! By Caroline LaManna Plasmids 101: What is a plasmid? By Margo...loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by responding...and improved. The CRISPR posts written by our guest bloggers, the experts in the CRISPR/Cas field, have ...Design Your gRNA for CRISPR Genome Editing By John Doench (Guest Blogger) Plasmids 101: Origin of Replication...Melina Fan Plasmids 101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian...Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids 101: Yeast Vectors...as seen through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and...

  8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post
    ...editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series Resources...following components: A plasmid in the host bacterium expressing dCas9 A plasmid in the host bacterium ...base editors for your research Read our CRISPR guide Find plasmids for your research ...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a... Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier this month...the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to the DNA to induce... of gene III to create infectious particles. A plasmid-based genetic circuit in the bacteria links the...

  9. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post
    ... on Addgene.org Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated...additional, unique facets of CRISPR DMD therapy. Long et al. show that AAV-CRISPR does not cause obvious germline...directions and obstacles to clinical CRISPR editing Given the success of the CRISPR-mediated exon skipping approach...To bring DMD CRISPR therapy closer to the clinic, much work is still needed. First, CRISPR delivery must... to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the speed...Majoros WH, Reddy TE, Gersbach CA. Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin...long period of time. High fidelity Cas9s such as eSpCas9 and SpCas9-HF should be explored to reduce off-target...

  10. Top Requested Plasmid of 2017 - pMD2.G

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    Blog Post
    ...discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but... deliver Cas9 and other genes to mammalian cells. For this reason, the top requested plasmid of 2017 is...envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin...ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the full, searchable list...to check out the Trono Lab website. If you have plasmids you’d like to deposit with Addgene, be sure to...

  11. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...single plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all...anyone who has worked with individual plasmids knows, some plasmids amplify well and others don’t. The size...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated...Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR libraries...Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...pooled library, which contains a collection of plasmids in a single tube, or a pre-made viral prep library...

  12. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post
    ...A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage combined with Gibson assembly...mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning...use of Gibson assembly combined with the popular CRISPR technology and is described in a recent publication...restriction enzyme or PCR to make linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave...second example of using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015)...chromosome (up to 100 kb) were specifically cut out via CRISPR and then assembled into a vector using Gibson assembly...homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent the need to PCR ...

  13. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    Blog Post
    ...consensus on the best CRISPR software tools yet. As a genome editing tool, the CRISPR/Cas9 technology has been...Read Our CRISPR Guide to Catch up on All the CRISPR Basics Read Other Blog Posts about CRISPR Find validated...in the first place? How CRISPR innovation affected software development CRISPR, like the miRNA bubble ... think we are currently at in the CRISPR software space. The CRISPR research community hasn't had a chance...pool of CRISPR software embodies solutions that facilitate most experimental applications in CRISPR engineering...type of CRISPR experiment being pursued. You should also look at the glossary section of the CRISPR Software...malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system." Nature biotechnology(2014). PubMed PMID...

  14. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand... made comparisons between CRISPR components expressed from different plasmid backbones. They noted that...comparisons of CRISPR parts. The Fragmid system was used to assess how different lentiviral plasmid backbone...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...targetability and effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique...unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing...

  15. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

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    Blog Post
    ...Browse gRNA Design Tools Read Our CRISPR Guide Pages Find CRISPR Plasmids  ...120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016). ...not generated from a plasmid. If you, for example, prefer to deliver your CRISPR components as RNPs (ribonucleoproteins...sequence, Addgene plasmid ID (if we have one associated with the gRNA), application, cas9 species, PubMed...Design Your gRNA Use the CRISPR Software Matchmaker Browse Alll of Our CRISPR Posts Additional Resources...visualize, nick, purify, tag, scaffold, CRISPR-display, and dCas9-FokI. This table is both sortable and .... Many of the gRNAs contained within these plasmids have been used successfully in peer reviewed articles...

  16. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of ...Improvement of pCVD442, a suicide plasmid for gene allele exchange in bacteria." Plasmid 51.3 (2004): 246-255. PubMed...15109831. Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with lambda red recombineering enables... on the Addgene blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda...genomes using exogenous enzymes: Lambda red and CRISPR Developed over the past 20 years, Lambda Red recombineering...exogenous enzymes, require multiple transformation and plasmid curing steps, and can generate off-target mutations...a mutated allele introduced from a conjugative plasmid replaces the chromosomal copy. This is a versatile...

  17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post
    ...Read our plasmids 101 blog series Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection...-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method...developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish...systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these...collection Read our molecular biology reference Find plasmids for your research ...

  18. Our Most Popular Blog Posts from 2019

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    Blog Post
    ...biology over the past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent proteins, careers...blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage...feature some recently deposited plasmids every quarter. The “hot plasmids” from last March were quite popular...behalf of scientists. 9. Plasmids 101: Positive and Negative Selection for Plasmid Cloning by Jennifer Tsang...and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique from David...more control and flexibility to CRISPR editing. Using a fusion between Cas9 and reverse transcriptase and...Addgene news Find the most recent plasmid blog posts Find the most recent CRISPR blog posts Resources at Addgene...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ... using CRISPR/Cas9 to edit a gene, the expression of Cas9 is no longer necessary. However, Cas9 can be... like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing ...integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details on how to...lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long...this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically...production of lentiviral particles.  The envelope plasmid typically encodes the vesicular stomatitis virus...

  20. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.        A retroviral mouse CRISPR knockout library...dependent promoter systems. Find these CRISPR activation plasmids at Addgene. Liu et al., Nature Communications...library Article contributed by Alycia Bittner CRISPR pooled libraries are popular tools for genetic screens...method used to introduce the gRNAs into cells. Most CRISPR pooled libraries have been created in lentiviral...by lentivirus, and thus screening with available CRISPR libraries was not possible. Primary murine T cells...
Showing: 141 - 160 of 274 results