We narrowed to 209 results for: dap

1. Plasmids 101: Cre-lox

   Type

   Blog Post

   ...natural viral life-cycle, and researchers have adapted the components for use in genome manipulation. ...

2. Hot Plasmids February 2024

   Type

   Blog Post

   ...by co-incubation of Cas-RNP and assist peptide. Adapted from Zhang et al. 2023 with permission.   Find...

3. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

   Type

   Blog Post

   ...methylation, leaving the target CpG unmethylated. Adapted from Sapozhnikov and Szyf 2021.   Let me leave...

4. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   ...indirect ELISA (more details below) can also be adapted to a competitive format. Figure 2: Overview...

5. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   ...library. This PCR step also adds the necessary adapter sequences for Illumina sequencing as well as unique...

6. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   ...which are essential for assembly of the virion. Adapted from Lee et al., Adenovirus-mediated gene delivery...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   ...protein-protein interactions. We’re excited to see how you adapt similar FLINC biosensors to work in your lab. Find...

8. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   ...locomotion. Mice may need to be given some time to adapt to a testing apparatus. General stress levels of...

9. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   ...Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered in plants to conditionally...

10. Mouse Modeling, Part 1: Genetically Engineered Mice

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    Blog Post

    ...mouse models provide researchers with useful and adaptable tools to perform basic and preclinical research...

11. Kit Free RNA Extraction

    Type

    Protocol

    ...Isopropanol or Lithium Chloride. This protocol was adapted from (Link opens in a new window) Chomczynski P...

12. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    ...our requestors. However, we can again see quick adaptation and steady use of all types of libraries, once...

13. Plasmids 101: Degron Tags

    Type

    Blog Post

    ...to ubiquitination and proteasomal degradation. Adapted from Nabet et al., Chemical Biology, 2018.  ...

14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...through error-free homologous recombination (HR). Adapted from an image provided by Manuel Gonçalves; made...

15. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

16. Plasmids 101: Knockout/Knock-In Plasmids

    Type

    Blog Post

    ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

17. Plasmids 101: 5 factors to help you choose the right cloning method

    Type

    Blog Post

    ...Gateway cloning protocol is fairly flexible and adaptable to cloning different types of DNA, such as cDNA...

18. Plasmids 101: Protein Expression

    Type

    Blog Post

    ... grown in well-defined media and can be easily adapted to fermentation allowing for large-scale, stable...

19. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

20. Plasmids 101: Optimizing Plasmid Yields

    Type

    Blog Post

    ...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...