We narrowed to 371 results for: fus

...of traditional tracing compounds is that their diffusion across the synaptic cleft is usually severely ...small (20 nm) in size which makes them capable of diffusing in the extracellular space; they also have low...there is always a risk that viral particles will diffuse long distances within the extracellular space along...

...surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence in PLoS ...neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking to delve...

...Plant base editing with a Cas9-adenosine deaminase fusion Caixia Gao’s lab has developed a new plant base...from an evolved tRNA adenosine deaminase that is fused to a nicking Cas9. This base editor can be used ...

... is fused to a translational repressor. In principle, however, designer PUF proteins can be fused to other...

... and A-to-G base editors into one protein. They fused an activation-induced cytidine deaminase (AID) and...adenosine-to-inosine (A-to-I) RNA editor called REPAIR by fusing catalytically inactivated RNA-targeting Cas13 (...

... e67902. Many imaging studies rely on direct fusion of fluorescent proteins (FPs) to a yeast gene of...epitope tag for creation of your own unique protein fusions. This collection is ideal for tandem affinity purification...

...making fusions, see this blog post from Joachim Goedhart: Fluorescent Proteins 101: GFP Fusion Proteins...

...The first functional SB system was synthesized by fusing and modifying various extinct transposases. Improvements...is made up of a fragment of the RPL13a promoter fused to a region of the RPL41 gene, to drive the selection...

...these applications, however, nuclease-dead Cas9 is fused to a transcriptional activator or repressor to create...libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface...

... common protein-only transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of...discovered in the HIV protein Tat. Although CPP fusions can translocate a cell membrane, the presence of...

...lab first thought to enable MBE by using Cas12a fused base editors (dCas12a-BE). However, the poor editing...efficiencies they found led them back towards Cas9 fused base editors (nCas9-BE), as Cas9 is mechanistically...

...useful for studies in non-human primates, where fusion fluorescent proteins can affect DREADD trafficking... (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid...

...in human cells (Ma et al., 2015). To do so, they fused a different fluorescent protein to various Cas9 ...Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized to specific loci based on the guide RNA...

...of dCas9, a gRNA fused to an RNA binding motif, and a RNA-binding protein-bEFP fusion. Thus, the bEFPs...

...with other pathways. Since these effectors are diffusible, they’re also not suitable for spatially limited...light-sensitive two-component system (TCS). Levskaya et al. fused a photosensory domain from cyanobacteria to a common...

...not appropriate for mammalian selection – do not confuse this with G418 (aka Geneticin). Neomycin should...mammalian expression – instead use G418. This can be confusing since the neo/kan gene confers G418 resistance...

...luminescent reaction when they come together. By fusing each luciferase subunit to other proteins, researchers...A luciferase and a fluorescent protein are each fused to one of the proteins being tested for interaction...

... Screens and selections The common goals The confusion in differentiating between a screen and a selection...inappropriately categorized as screens! Why the confusion? The commonality between these methods is that...

...2021.    BLITZ uses the biotin ligase TurboID fused to a conditionally stabilized GFP-nanobody rather...rather than a specific protein of interest. This fusion protein can then be co-expressed in essentially any...

...cryopreserved hybridomas, immortalized cells created by fusing an activated B cell that produces the antibody ...light (VL) and variable heavy (VH) chain sequences. Fusion PCR was performed to create an amplicon that  was...