Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 162 results for: lentiviral vector

Showing: 141 - 160 of 162 results

  1. Neurodegeneration Research Collection

    Type
    Collection
    ... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...motor neuron-specific reporter and a PAX7 inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

  2. CRISPR References and Information

    Type
    Collection
    ... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for ...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and...PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...

  3. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...virus (AAV) and lentivirus are now available from Addgene. While super useful, these vectors require a bit...to generate new knockout mice, using adenoviral vectors to deliver IGF receptors to the brain, and probing...individual labs to make efficiently. For select viral vectors in the repository, you can instead have Addgene... into neuroscience, you could look to our viral vector pages on the Addgene website and on the Addgene...

  4. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...in viral vectors, but easily fit in a TE. Transposons are also less likely than viral vectors to induce...advantage over viral vectors (~5 kB payload for AAV and ~8 kB payload for lentivirus). Large cargos, such...transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three different ... in human cells at rates similar to retroviral vectors. The hyperactive version of the SB transposase,...efficiency in human cells similar to retroviral vectors. The hyperactive PB transposase (hyPB) is ~10-fold...transfer Transposons are an alternative to viral vector delivery of transgenes, like for iPSC reprogramming...

  5. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...number of gRNAs that can be packaged into viral vectors for potential in vivo applications or multiplex...unprecedented number of genes with a single viral vector.   Fig. 1: Schematic of multiplex gene ...is scalable and efficient in plasmid and viral vectors. In applications when repetitive tRNA sequences...deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes ...

  6. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    ...terminator will suffice. Many commercial expression vectors use double terminators to reduce unwanted translation... handled with care (4). For this reason, viral vectors often employ other non-poly(A) transcript-stabilizing...Expression of Transgenes  Delivered by Retroviral Vectors. Journal of Virology. 1999. PubMed PMID: 10074136... Substantially Increases Expression Levels of Lentivirus-Delivered Transgenes but Has the Potential to...

  7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...polymerase pLKO.1 - TRC Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used by the ...plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus with a polyethyenimine (PEI) transfection...preparation Lentivirus Titration by ddPCR Use ddPCR to measure the physical titer of your lentivirus preparation...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...measurements Colony Formation Titering Assay for Lentivirus Quantify virus by counting antibiotic resistant colonies...colonies Generating Stable Cell Lines with Lentivirus Genomically integrate your DNA sequence into a cell...

  8. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, ...Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  9. Lentiviral Vector Guide

    Type
    Guide
    ...Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by removing...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

  10. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila ...Clear Filters Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs Adamson...TP53 Exon Mutation Libraries 229137–229140 Donor Vector Human Stiewe N/A Varies 1,910–3,034 per exon Human...cells). Most CRISPR libraries require the use of lentivirus to deliver the gRNA/Cas9 to target cells. Therefore...Therefore, you must be equipped and authorized to use lentivirus in your lab. Once you have determined that CRISPR-based...

  11. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...by Limiting Dilution You may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral... plasmid #52962 ) and is described in Improved vectors and genome-wide libraries for CRISPR screening.... pool of stable cells. Transducing cells with lentivirus results in a heterogenous polyclonal population...protocol for generating stable cell lines with lentivirus . Day 0: (optional) Seed cells for the generation...protocol for generating stable cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is...

  12. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral...Much like lentiviral vectors, gamma-retroviral vectors integrate at random locations throughout the genome... for lentiviral vectors, but a similar method can be applied when using gamma-retroviral vectors. Gene...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...Gamma-Retroviral Vectors Vector Production Integration Pseudotyping Common Uses Safety Resources Plasmid ...

  13. Plan Your Experiment

    Type
    Guide
    ...viral vectors and their production, see our viral vector guides . Lentiviral vectors Lentiviral vectors... in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. ...delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors takes... CRISPR plasmids . Delivery Using Viral Vectors Viral vectors are a great option, as they offer high expression...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide.... Both lentiviral and AAV vectors are safe to use in the lab, but AAVs exhibits the lowest immunogenicity...

  14. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure...our CRISPR guide pages Find ready-to-use Cas9 lentivirus ...

  15. Guide to Using Pooled Libraries

    Type
    Guide
    ...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells...genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts featuring ...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

  16. CRISPR Guide

    Type
    Guide
    ...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

  17. Molecular Biology Reference

    Type
    Guide
    ...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...specifically engineered plasmids, commonly referred to as vectors, which have become possibly the most ubiquitous...facilitate the cloning of DNA fragments. Cloning vectors tend to be very simple, often containing only a...expression (for the purposes of gene study). Expression vectors must contain a promoter sequence, a transcription...the transcription process to stop. An expression vector can also include an enhancer sequence which increases... amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Douglas Kim , GENIE Project Calcium Bicistronic vector expressing GCaMP6 and mRuby2 for imaging neural... Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional Payloads to Cells... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal...mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging PI Return to ...

  19. Plasmid Collections

    Type
    Collection
    ...Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific community that has...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...Gateway® Entry vectors from the labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE...systems. The MXS-chaining kit includes chaining vectors with multiple cloning sites and standardized building... reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single...
Showing: 141 - 160 of 162 results