We narrowed to 881 results for: lic

...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...while the SnapGene powered map is on the right (click here to see the new map in action). Read on to learn...simple but powerful display improvements, when you click on any plasmid map, you also have the ability to...analyzer will allow you to dive into the details. Click on the “View all sequences” button to find all available...For more immediate, high-level analysis, you can click directly on the “Analyze Sequence” button to pull...Maps This is the first tab you’ll land on after clicking the “Analyze Sequence” button. The maps hosted...and a description of its function. You can also click one location in the plasmid and then a second location...

...led to their application for basic and translational research.  Basic research applications of scFvs: ...made up of glycine and serine with dispersed hydrophilic residues for increased solubility (Monnier et...blood clearance, which is useful for imaging applications; better tissue penetration which is useful for... for therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to...from blood can be a drawback for therapeutic applications where longer retention times often increase ...since it’s genetically encoded.   Translational applications of scFvs: Just like antibodies, there are many...2019).Many of the scFvs designed for oncology applications are reformatted as bispecific molecules which...

... and comment online! Want the general public (say, policymakers or community organizations) to use your...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...scientific manuscripts shared by the authors on a public server independent from peer-reviewed publishing...courtesy of Henry Mühlpfordt, under a CC BY-SA license. Many scientists and advocates have argued ...increasingly growing time from submission to publication. It takes many months, often more than a year...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...and updated by the authors over time, providing a public record of changes, critiques or endorsements. In...

...epigenetic modifications across the genome) has been implicated in disease states, and targeting the epigenome...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety...small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L...doi.org/10.1038/nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black JB, Chellappan M...doi.org/10.1038/nbt.3853 Find plasmids from this publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, ...10.1038/ncomms16026 Find plasmids from this publication at Addgene. Liu XS, Wu H, Ji X, Stelzer Y, Wu.../j.cell.2016.08.056 Find plasmids from this publication at Addgene. Maeder ML, Angstman JF, Richardson...

...searches, such as clicks to read the abstract or full text of the paper, publication date, a relevance...show all publications by an author, total number of citations, and the number of publications that heavily...that term. You can also set up alerts for new publications from a particular author. Saving papers Papers... work, so it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 ...organizations, and universities often tweet about publications in the fields of research they support. Monitoring...Artificial Intelligence. Semantic Scholar analyzes publications and figures using machine learning to extract...your search to a specific window of time for publication. To help you quickly identify papers with greater...

...the OpenWorm website. microPublication microPublication is a unique publication for the worm community...into larger publications and would otherwise be lost in lab notebooks forever. microPublications are peer-reviewed...aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of mRNA are important ...normally one figure and can be negative and/or even replicated studies to show reproducible results. This a ...referenced. In addition the data published in microPublications is curated and added to other databases including...including wormbase and flybase. microPublication is run and maintained by members of the worm community...introducing point mutations that was published in a microPublication (Schwartz and Jorgensen, 2018).  These are...

...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice. Target and application should be considered The first question you may... a strong signal and to work in a variety of applications. The desired specificity of your secondary antibody...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...mouse IgG2a and rat IgG1 antibodies in the same application, using a Goat anti-mouse IgG2a cross-adsorbed...

...Organizing a science conference for the public How does one organize a public scientific conference? As a member...DayCon is a one-day conference aimed at the general public that provides an accessible introduction to various...the communication gap between scientists and the public and help make science more transparent. Second,... their communication skills and engage with the public. The organization runs a wide variety of programs...conference-style experience that was accessible to the general public both in terms of cost and content level. Sponsorship...science outreach can be, both for scientists and the public. For students, these opportunities to improve our... alternative careers in science. In return, the public receives trustworthy, intelligible scientific resources...

... tools for familiar applications. But how does one invent a tool for applications that don’t even exist... Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just that ...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible. But ...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that...reproduced from Frank Hayward et al. 2024 under CC-BY license. They did more experiments to see if any small...reproduced from Frank Hayward et al. 2024 under CC-BY license.   Video 1: Fluorescence movie of E. coli...

...intended application - Remember, just because your antibody performs as expected in one application does ...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...intentionally generalizable and not specific to certain applications or antibody targets. That said, not every strategy...will be suitable for every antibody or every application. The goal is to show that your antibody has been...strategy to use will depend on your intended application and available resources.     Figure 1...confident in your data. This is a good option for applications like immunohistochemistry on human tissues, ...alternative translation start sites or alternative splicing that could lead to some form of the target protein...

...Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications Organic...labeled through click chemistry. Although these multi-component systems can be complicated to implement,...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...adapted from (Bottanelli et al., 2016) under CC-BY license. Combining orthogonal self-labeling tags on...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...reused from (Ulrichs et al., 2023) under CC-BY license. But these tags aren’t limited to fluorescent...

... ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate replication. ITRs are...binding site for Rep78 and Rep68 to initiate replication and a terminal resolution site that serves as...are thought to be self-repaired during the AAV replication process (Chen et al., 2004). Mutations in the... Rep proteins are important for viral genome replication, these mutations can affect transduction efficiency... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and... and hairpin structures, ITRs are prone to replication errors and disruptive mutations and truncations...

...RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the impacts...Line Mutation (DNA) Background Strain Purpose Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at...DNA) Mutation (protein) Sex Source of Material Publication PI N126I A377T N126I M Fibroblasts & iPSC (Link...plasmids! To get started with your deposit, please click here ....

...because of its relative simplicity as a gene editing system. Despite that simplicity, its accessibility outside.... Reproduced from Wu et al. (2025) under CC BY license. These datasets (in healthy mice and a Huntington's...models, showing that this transfer increases the metabolic and metastatic capacity of cancer cells. This ... Confocal image of CA2 neurons in a mouse brain slice expressing PinkyCaMP; scale bar 50 µm. Reproduced...from Fink & Imai et al. (2024) under CC BY-NC-ND license. B) Confocal images showing fore-, mid-, and hindbrain...Zhou, Zhu, Eom, Fang, et al. (2025) under CC BY license. Both groups demonstrated their sensors in...in a variety of neuronal imaging applications, including confocal and widefield imaging, two-photon microscopy...

...Require Sialic Acid Binding for Hemagglutination and Efficient Transduction but Differ in Sialic Acid Linkage...receptor Coreceptor AAV1 α2,3-/α2,6 N-linked sialic acids1 AAV receptor (AAVR)2   AAV2 Heparan...Laminin receptor (LR)3 CD93 AAV5 2,3-N-linked sialic acid4 AAV receptor (AAVR)2 Platelet-derived...(PDGFR) α and β3 AAV6 α2,3-/α2,6 N-linked sialic acids1 Heparan sulfate proteoglycan (HSPG)3 AAV...vessel barriers. It is especially popular for applications targeting liver tissue. In addition, it has ...the blood-brain barrier, making it useful for applications targeting the CNS (Issa et al., 2023). Additionally...treatment, which can pose problems for gene therapy applications. Cross-reactivity of AAV serotypes varies by...

...between mGold2s and mGold2t depending on the application. Figure 1: Super-resolution imaging...from Lee & Lai et al. (2025) under CC BY-NC-ND license. If you’re currently using mVenus or mCitrine...proof-of-concept, they believe that the strategy will be applicable with other TnpBs and in other plant species, ...available for tracking cell lineage, different applications require careful selection or design to ensure...Reproduced from Singh et al. (2025) under CC BY license. Libraries are available with T-Sapphire, ...for gene therapy and ex vivo cell engineering applications and is especially well-suited for lung cells...tool for circuit tracing and other neuroscience applications (Figure 4). Check out our blog post on AAV serotypes...

...suppliers, and public health and medical organizations are working to help educate the public about proper...containing a phage origin of replication that can be packaged into replication-incompetent phage particles...Beisel et al., licensed under a Creative Commons Attribution 4.0 International License.   Improving...essential processes: cell wall synthesis, DNA replication, or protein synthesis. Because they target such...mammalian viral vectors in that the vectors are replication-deficient. Thus, a very large number of phagemids...CRISPR antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy...bacterial host range. To further potential clinical applications, Ando et al. recently engineered synthetic phages...

...for both transient and stable gene expression applications in plants. This smaller, easily customizable...Agrobacterium are termed binary vectors because they must replicate in two species: Agrobacterium and E. coli. Like.... coli. However, they also need to be able to replicate in Agrobacterium to ensure that they are maintained... Thus, binary vectors contain two origins of replication, one each for E. coli and Agrobacterium, along...bases each. By switching the E. coli origin of replication and codon-optimizing the Agrobacterium ORI, they...constitutively active M24 promoter has been modified with duplicated enhancer domains and characterized in studies...these properties of pSiM24 are suitable for many applications, the plasmid can also be customized due to its...

...to disparate physiological effects and complicate any metabolic study. The Ralser lab has created a unique.... Seeliger MA, Young M, Henderso MN, Pellicena P, King DS, Falick AM, and Kuriyan J. Protein Sci. 2005...brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We...Wu Lab library can be found in the associated publication. Ma, et al. Cell Rep. 2015. PubMed PMID: 26190106...and ubiquitous reporter assay. In their recent publication, Kroeze et al. describe their enhanced TANGO .... Each gene was chosen to represent the primary splice variant in 38 types of human cancer. Many of the...Sanger sequencing, respectively. The speed and simplicity of GMAP allows scientists to easily design, generate...

...emission wavelengths, which are pivotal in their application in biotechnology (Grimm & Lavis, 2022). These... substrates/peptides and antibodies. Example applications of this include coupling to an antibody, whereby...typically bind to. Stability of fluorescence: For applications that involve ranges of pH, and significant lengths...light, is another factor to consider. If your application requires longer periods of exposure, you’ll want...have shown that different fluorescent ligands can elicit varying degrees of β-arrestin-2 recruitment to ...descriptions, or technical manuals. Specific applications and matching dye/ligand choices For effective...beyond the diffraction limit of light. Dyes like silicon-rhodamine or Janelia Fluor (JF) dyes have specific...