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We narrowed to 876 results for: lic

Showing: 141 - 160 of 876 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...experiment is targeted amplicon sequencing. The information from targeted amplicon sequencing is highly ...have been widely adopted due to their relative simplicity and low cost. The problem with these assays is... genome for off-target sites irrespective of in silico prediction. These techniques differ in important...heterogenous populations, low sensitivity Targeted Amplicon Sequencing Provided 0.01% (variable) Sequence-...rate and the repair product sequence. Targeted amplicon sequencing doesn’t tell the whole story ...These strategies can be used in concert with in silico prediction to create a more comprehensive list ...unbiased detection isn’t as sensitive as targeted amplicon sequencing. For example, GUIDE-seq seems to have...

  2. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ...targeting the pMB1 replicon on pTargetF (Jiang et al., 2015). In addition to the pMB1 replicon, pTargetF contains...PrhaB, 2) changed the replicon of pCas into a non-temperature-sensitive replicon, and 3) added the sacB... the S. pyogenes Cas9, a temperature-sensitive replicon for self-curing, an arabinose inducible lambda-red... expression of the sgRNA that targets the pMB1 replicon, resulting in cutting of the plasmid. At this ... the gRNA on pCas that is specific to the pMB1 replicon of pTargetF may exhibit a more serious leaky expression...serve to interrupt genes (or gene clusters) in metabolic engineering. So far, we’ve used the MUCICAT system...when compared to the industrial strain. In our metabolic engineering case study of N-acetylglucosamine ...

  3. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...cutters. You can also sort enzymes by clicking on column heads. Clicking on a particular restriction enzyme...while the SnapGene powered map is on the right (click here to see the new map in action). Read on to learn...simple but powerful display improvements, when you click on any plasmid map, you also have the ability to...analyzer will allow you to dive into the details. Click on the “View all sequences” button to find all available...For more immediate, high-level analysis, you can click directly on the “Analyze Sequence” button to pull...Maps This is the first tab you’ll land on after clicking the “Analyze Sequence” button. The maps hosted...and a description of its function. You can also click one location in the plasmid and then a second location...

  4. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...led to their application for basic and translational research.  Basic research applications of scFvs: ...made up of glycine and serine with dispersed hydrophilic residues for increased solubility (Monnier et...blood clearance, which is useful for imaging applications; better tissue penetration which is useful for... for therapeutic and imaging applications; and reduced immunogenicity when administered in vivo due to...from blood can be a drawback for therapeutic applications where longer retention times often increase ...since it’s genetically encoded.   Translational applications of scFvs: Just like antibodies, there are many...2019).Many of the scFvs designed for oncology applications are reformatted as bispecific molecules which...

  5. Summer SciComm: Preprints

    Type
    Blog Post
    ... and comment online! Want the general public (say, policymakers or community organizations) to use your...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...scientific manuscripts shared by the authors on a public server independent from peer-reviewed publishing...courtesy of Henry Mühlpfordt, under a CC BY-SA license. Many scientists and advocates have argued ...increasingly growing time from submission to publication. It takes many months, often more than a year...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...and updated by the authors over time, providing a public record of changes, critiques or endorsements. In...

  6. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...epigenetic modifications across the genome) has been implicated in disease states, and targeting the epigenome...to specific DNA sequences. Non-editing CRISPR applications direct catalytically dead dCas9 fused to a variety...small DNA methyltransferase from the prokaryote Mollicutes spiroplasma (M. Sss1) (termed MQ1.) The Q147L...doi.org/10.1038/nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black JB, Chellappan M...doi.org/10.1038/nbt.3853 Find plasmids from this publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, ...10.1038/ncomms16026 Find plasmids from this publication at Addgene. Liu XS, Wu H, Ji X, Stelzer Y, Wu.../j.cell.2016.08.056 Find plasmids from this publication at Addgene. Maeder ML, Angstman JF, Richardson...

  7. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...searches, such as clicks to read the abstract or full text of the paper, publication date, a relevance...show all publications by an author, total number of citations, and the number of publications that heavily...that term. You can also set up alerts for new publications from a particular author. Saving papers Papers... work, so it’s also a good place to look for publications. Bonus: papers have to be summarized in 280 ...organizations, and universities often tweet about publications in the fields of research they support. Monitoring...Artificial Intelligence. Semantic Scholar analyzes publications and figures using machine learning to extract...your search to a specific window of time for publication. To help you quickly identify papers with greater...

  8. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...your experiment (all well validated for your application, of course!) When the stars are aligned and the...factors that affect isotype choice. Target and application should be considered The first question you may... a strong signal and to work in a variety of applications. The desired specificity of your secondary antibody...affect my experiment?” Well, it depends on the application and target. If you are running a basic western...target is expressed at very low levels or your application requires a high degree of affinity, then species...considered when choosing an antibody. In some applications probing species-on-species, i.e. using a mouse...mouse IgG2a and rat IgG1 antibodies in the same application, using a Goat anti-mouse IgG2a cross-adsorbed...

  9. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...the OpenWorm website. microPublication microPublication is a unique publication for the worm community...into larger publications and would otherwise be lost in lab notebooks forever. microPublications are peer-reviewed...aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of mRNA are important ...normally one figure and can be negative and/or even replicated studies to show reproducible results. This a ...referenced. In addition the data published in microPublications is curated and added to other databases including...including wormbase and flybase. microPublication is run and maintained by members of the worm community...introducing point mutations that was published in a microPublication (Schwartz and Jorgensen, 2018).  These are...

  10. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ...Organizing a science conference for the public How does one organize a public scientific conference? As a member...DayCon is a one-day conference aimed at the general public that provides an accessible introduction to various...the communication gap between scientists and the public and help make science more transparent. Second,... their communication skills and engage with the public. The organization runs a wide variety of programs...conference-style experience that was accessible to the general public both in terms of cost and content level. Sponsorship...science outreach can be, both for scientists and the public. For students, these opportunities to improve our... alternative careers in science. In return, the public receives trustworthy, intelligible scientific resources...

  11. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... tools for familiar applications. But how does one invent a tool for applications that don’t even exist... Andrew York, Maria Ingaramo, and their team at Calico Life Sciences recently set out to do just that ...phenomenon, improve the tool, and develop new applications for magnetoresponsive proteins. Motivated by...organisms) are opaque, which makes most biomedical applications of these tools difficult or impossible. But ...tool with magnets would enable new research and applications that don’t yet exist, but it wasn’t clear that...reproduced from Frank Hayward et al. 2024 under CC-BY license. They did more experiments to see if any small...reproduced from Frank Hayward et al. 2024 under CC-BY license.   Video 1: Fluorescence movie of E. coli ...

  12. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top ...top Plant ID Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection...

  13. Antibodies 101: Validation

    Type
    Blog Post
    ...intended application - Remember, just because your antibody performs as expected in one application does ...strategies for antibody validation in different applications. Here, I want to highlight the general suggestions...intentionally generalizable and not specific to certain applications or antibody targets. That said, not every strategy...will be suitable for every antibody or every application. The goal is to show that your antibody has been...strategy to use will depend on your intended application and available resources.     Figure 1...confident in your data. This is a good option for applications like immunohistochemistry on human tissues, ...alternative translation start sites or alternative splicing that could lead to some form of the target protein...

  14. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert...Insert Promoter Selectable Marker PI Publication Return to top Yeast ID Plasmid Gene/Insert Promoter Selectable...Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR plasmids...

  15. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert Selectable Marker PI Publication Return...insertions — meaning they may not be suitable for applications where orientation of the insert is important...

  16. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Few Complicated Non-natural amino acids 1 Various Many Complicated   Applications Organic...labeled through click chemistry. Although these multi-component systems can be complicated to implement,...having to clone a whole new construct for every application. Plus, the photophysical properties of chemical...advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-cell labeling strategies...adapted from (Bottanelli et al., 2016) under CC-BY license. Combining orthogonal self-labeling tags on...self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2). If you’...reused from (Ulrichs et al., 2023) under CC-BY license. But these tags aren’t limited to fluorescent...

  17. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ... ITRs serve as the origin of replication and are required for replication, packaging, and vector persistence...viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate replication. ITRs are...binding site for Rep78 and Rep68 to initiate replication and a terminal resolution site that serves as...are thought to be self-repaired during the AAV replication process (Chen et al., 2004). Mutations in the... Rep proteins are important for viral genome replication, these mutations can affect transduction efficiency... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and... and hairpin structures, ITRs are prone to replication errors and disruptive mutations and truncations...

  18. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...TCGATGAATTCGAGCTCG–3' ID Plasmid Selectable Marker Tags Publication FP Overexpression Plasmids for constitutive overexpression...marker. ID Plasmid Gene/Insert Selectable Marker Publication Additional Resources Addgene Kits MoClo-YTK Yeast.... L., von Wirén, N., Frommer, W. B. (2007). A cytosolic trans-activation domain essential for ammonium...

  19. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...Protocols & Resources Contents of Kit Original Publication Oligomerized pool engineering (OPEN): an 'open-source... in the Materials and Methods of your future publications. For your Materials and Methods section: "The...The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)" For your...

  20. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...because of its relative simplicity as a gene editing system. Despite that simplicity, its accessibility outside.... Reproduced from Wu et al. (2025) under CC BY license. These datasets (in healthy mice and a Huntington's...models, showing that this transfer increases the metabolic and metastatic capacity of cancer cells. This ... Confocal image of CA2 neurons in a mouse brain slice expressing PinkyCaMP; scale bar 50 µm. Reproduced...from Fink & Imai et al. (2024) under CC BY-NC-ND license. B) Confocal images showing fore-, mid-, and hindbrain...Zhou, Zhu, Eom, Fang, et al. (2025) under CC BY license. Both groups demonstrated their sensors in...in a variety of neuronal imaging applications, including confocal and widefield imaging, two-photon microscopy...
Showing: 141 - 160 of 876 results