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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 162 results for: mPA-GFP

Showing: 141 - 160 of 162 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...third-generation packaging plasmids. For a summarized comparison of second- and third-generation systems, please...have limited restriction sites or may only be compatible with certain cloning methods (such as a Gateway... so be sure to confirm your chosen plasmid is compatible with cloning methods available in your lab. When...lentiviral preference for transcribed genes. Studies comparing the lentivirus HIV and the gamma-retrovirus MMLV...

  2. Modular Cloning Guide

    Type
    Guide
    ...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...standardized modules. In the first assembly step, compatible Level 0 parts are directionally assembled into...Kit Plant Expression Wayne Parrott 48 plasmids compatible with the GreenGate Cloning System (Lohmann) to... metabolic engineering and synthetic biology, compatible with PhytoBrick parts. MoBiFC - Modular Bimolecular... expression in baculovirus or S. cerevisiae . Compatible with MoClo-YTK , Bac-to-Bac, and biGBac systems...Bacterial Expression Mikhail Shapiro 96 cross-compatible plasmids including 11 inducible promoters, 16...16 RBS strength variants, 8 tag-compatible RBSs, 2 secretion tags, 6 CDSs, 9 terminators, 4 nonfunctional...

  3. Optogenetics Guide

    Type
    Guide
    ...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 9(...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression cassette...halves of EGFP to recombine by homology directed repair, and resulting in the expression of EGFP. Using ...ordering the sgRNA is simple and easy. Several companies supply gRNA sequences, such as GenScript, ThermoFisher...order them yourself through whatever DNA supply company you currently use. You can also find many previously...strand annealing) took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid...pCAG-EGxxFP target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The...can be placed in multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized ...

  5. CRISPR Guide

    Type
    Collection
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became...it meets two conditions: The sequence is unique compared to the rest of the genome The target is present...throughout the genome, called off-targets, that can impact your experiment. There are many online tools available...effects Sniper-Cas9 - less off-target activity; compatible with truncated gRNAs to increase specificity ... offer high efficiency and low indel formation compared to HDR. Prime editing requires more optimization...) includes tnsA , tnsB , tnsC , and tniQ . In comparison, type V-K includes just tnsB , tnsC , and tniQ...

  6. Arf GTPase Family

    Type
    Collection
    ...EFA6) 5662 1024 Mammalian (pEGFP-C1) GEF Psd2 (EFA6C) 84249 771 Mammalian (pEGFP-C1), Gateway GEF Psd3 (EFA6D...proteins, which also serve effector roles). A compatible GEF, GTPase, and GAP set is referred to as a ...GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway GTPase Arl2 402 184 Bacterial (pET),...116984 1704 GAP Arap3 (CENTD3) 64411 1544 Mammalian (pEGFP-C2) GAP Acap1 (CENTB1) 9744 740 Mammalian (pFLAG-CMV2...Bacterial (pET) GAP Git1 28964 770 Mammalian (pcDNA3, pEGFP) GAP Git2 (PKL, CAT2) 9815 759 GAP ELMOD1 55531 ...GAP RP2 6102 350 Bacterial (pGEX4T), Mammalian (pEGFP-N3), Gateway GEF Arfgef1 (BIG1, ARFGEP1) 10565 1849...398 Gateway GEF Cyth2 (ARNO) 9266 400 Mammalian (pEGFP-C1), Gateway GEF Cyth3 (ARNO3, GRP1) 9265 399 Gateway...

  7. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable change in fluorescence...better, multiple other labs have reproduced their EGFP findings since the initial report in 2023.)   ...   Figure 1: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT...proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable one-component... the proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random... semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of...-up studies. “We don’t have a vision of passive impact,” he says, noting that his team regularly shares...

  8. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...biological properties of new fusions can be compared with mEGFP or mTurquoise2, which are established true...fluorescent protein. In the jellyfish Aequorea victoria, AvGFP forms a homodimer. In corals, the red fluorescent...detect weak dimerization tendency, as is present in EGFP. Constantini et al, (2012) developed a cell-based...fluorescent protein is monomeric without the accompanying data to prove it is worthless. The OSER assay...that you will be using. If you do a head-to-head comparison right, it will be valuable information for the... are engineered can be shared to simplify the comparison by others. Together, we can build a valuable ...

  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional...) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for targeted cytosine methylation...methylation in mammalian cells. Co-expression markers EGFP and PuroR enable sorting and selection of transduced...Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9 to a small DNA methyltransferase...epigenome-editing tools. pLV hUbC-dCas9-MQ1(Q147L)-EGFP is also available for lentiviral transduction. Lysine-specific...

  10. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ..., and disease. In order to expand their model’s impact, organoid users have recently begun to develop ...circularly permuted green fluorescent protein or cpGFP) to the petunia SL enzymatic receptor DAD2. SLs ...which modulates the fluorescence intensity of the cpGFP without altering that of the internal control (Fig...these novel biosensors could have a major economic impact.   Design and mechanism of action of the...

  11. Zebrafish Plasmid Collection

    Type
    Collection
    ...including Myc, HA, Flag, GST and eGFP epitopes. Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative ...Sauka-Spengler Lab. A genetic binary system for cell- and compartment-specific biotin labeling in zebrafish. pGCS Vector...

  13. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Delivering gRNA and Cas9 using adenoviral vectors As compared to other viral vectors, AdVs can provide a large... pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6.gRNAGFP. Gonçalves says that advantages of AdVs include...guide RNA constructs, pAdSh.U6.gRNAS1 and pAdSh.U6.gRNAGFP, and also Adeno Cas9 and Adeno EA. Or if you're...

  14. TALEN Engineering

    Type
    Collection
    ...Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL Assembly TALEN...

  15. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...fluorescent proteins, how to do a head-to-head comparison of fluorescent proteins and how to make a well-informed...your selection on a small-scale head-to-head comparative study under conditions that best mimic the intended... QY (EC*QY, sometimes normalized to the value of EGFP). The higher the number, the higher the theoretical...protein properties and provides a quick way to compare fluorescent proteins, e.g. see Chudakov et al (...is of no use. Therefore, it is more sensible to compare fluorescent proteins based on their practical brightness...promising fluorescent proteins and to determine and compare their practical brightness in a system closely ...application. In figure 2 we provide an example of the comparison of the practical brightness of two orange fluorescent...

  16. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible with the other tools...based on an antibody-like protein AMPA.FingR (AF) that binds to AMPA receptor subunit GluA1 found at excitatory...especially among plant biologists, due to their compact size and ability to generate heritable edits (learn...third of the size of Cas9). Figure 2: Comparison of TnpB (A) and Cas9 (B) genome editors. Effector...These libraries will be useful for studying the impacts of other cancer mutations and non-genetic diversity...into the synapse. Once AF binds to the postsynaptic AMPA receptor and is endocytosed, the Cre recombinase...synaptobrevin 2; At = ALFA-tag; BACE = β-secretase; AF = AMPA.FingR; FingR = fibronectin intrabody generated with...

  17. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...Protein Property Visualization allows for visual comparison of FPs and how they relate to one another. .  ...FPbase Fluorescence Spectra Viewer can be used to compare excitation and emission spectra when available,...relating to that specific protein (for instance: EGFP).  Users can “favorite” certain proteins, or create...you or your lab use frequently) for the sake of comparison or ease of retrieval.  These collection pages...store and represent within-study photobleaching comparisons.  We very much appreciate feedback, so try out...

  18. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

    Type
    Blog Post
    .... This is cumbersome and also makes it hard to compare results across multiple targets because different...primary cells. Find plasmids for CAPTURE 2.0! When compared to the original CAPTURE, CAPTURE 2.0 had a ~14...sequence targeted in the experiment. Figure 2: Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE...lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs Number of loci targeted One ...regulation. When chromatin conformation data is compared with changes in gene expression, chromatin accessibility...

  19. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene... genes in this plasmid would be more difficult compared to if each gene were encoded in separate (and ...performed in the absence of some viral proteins (compared to viral infection). Therefore, the roles/functions...PMCID: PMC3586226. 4. Mühlberger, Elke, et al. "Comparison of the transcription and replication strategies...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    ... complex secondary structure. Today, multiple companies offer long single-stranded DNA production services...maximum Easi-CRISPR efficiency. Easi-CRISPR is compatible with standard microinjection and electroporation...commonly used cassettes, such as fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and...placed to mediate electroporation. Ohtsuka et al. compared traditional microinjection and i-GONAD knock-in...efficiency using a small ssDNA, and they found comparable efficiencies (52 vs 49%). In contrast, Easi-CRISPR...
Showing: 141 - 160 of 162 results