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We narrowed to 24 results for: mPA-GFP

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Showing: 1 - 20 of 24 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mPA-Emerald 487 509 400 Monomer (A206K) mPA-Emerald-N1 - Mammalian Expression mPA-Emerald-C1...Mammalian Expression sfGFP (superfolder GFP) 485 507 54 14 min Prone to dimerization sfGFP-N1 - Mammalian Expression...Expression EGFP 488 507 34 6 25 min Prone to dimerization pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian...Expression PA-GFP 504 517 400 14 Monomer (A206K) pPAGFP-N1 - Mammalian Expression pPAGFP-C1 - Mammalian... pRSETA-PAGFP - Bacterial Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 ...Brightness pKa Maturation Structure Plasmids Free Use GFP (fuGFP) 395 503 Monomer pUS252 - Mammalian Expression...high concentration. Proteins that are derived from GFP and contain the A206K mutation are monomeric at all...

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Gerlich 25999 LV-GFP Chromatin H2B GFP Elaine Fuchs 11680 H2B-GFP Chromatin H2B GFP Geoff Wahl 21217 ...61803 GFP-Rab7A Endosomes (late) Rab7a AcGFP Gia Voeltz 12605 GFP-rab7 WT Endosomes (late) RAB7 GFP Richard...Beta-catenin GFP Adherens junctions beta-Catenin EGFP Alpha Yap 67937 mouse E-cadherin GFP (423) Adherens...38277 pMXs-puro GFP-p62 Autophagosomes Sequestosome-1 EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI-1 Autophagosomes...emiRFP703 Vladislav Verkhusha 17650 GFP-HP1gamma Chromatin HP1 gamma GFP Tom Misteli 55068 mCherry-LaminA-C...118736 pBOB-CARMIL2 BH domain-GFP Plasma membrane CARMIL2 BH domain GFP John Cooper 118737 pBOB-CARMIL2...Gadella 50057 pLYS1-FLAG-MitoGFP-HA Mitochondria MCU EGFP Vamsi Mootha 49153 GFP-Mff Mitochondria outer ...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...agroinfection-compatible Tobacco mosaic virus (TMV)-based transient expression vector GFP Varies pMKO.1 GFP - Retroviral...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...fusing your protein to another protein, such as GFP, which allows you to visualize the cellular localization... cells, integrate into host genome pLenti CMV GFP DEST - Lentiviral Gateway destination vector for ...PGKpuro2ABFP - For retroviral delivery of one sgRNA pMKO.1 GFP - Retroviral shRNA expression Retroviral backbones...stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo DEST (719-1) - 3rd gen lentiviral Gateway destination...

  4. Control AAV Preps

    Type
    Collection
    ...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 116869 pAAV-CAG-H2B-GFP CAG H2B-GFP Constitutive...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 6, 8, 9, 11, rg*, PHPeB...PHP.eB, PHP.S Edward Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB ...Constitutive 1 Kiryl Piatkevich 214147 pAAV-hIBA1a-GFP-miR124T hIBA1a GFP Constitutive 5 Chun-Li Zhang 20299 pAAV-EF1a-double... Viviana Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Gordon Fishell..., 9, rg* Karl Deisseroth 122100 pAAV-EF1α1.1-GFP EF1a GFP Constitutive 2 Edward Boyden 128434 pAAV-Ef1a-fDIO-tdTomato...Jordane Dimidschstein 135631 pAAV-S5E2-GFP-fGFP E2 regulatory EGFP Constitutive 1, 9, PHP.eB Jordane Dimidschstein...

  5. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-1-PV2527 99039-AAV1 pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics...pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics Ed Boyden AV-5-PV3446 59170-AAV5 pAAV-Syn-Chronos-GFP Optogenetics...pAAV-hsyn-Jaws-KGC-GFP-ER2 Optogenetics Ed Boyden AV-8-PV3638 65014-AAV8 pAAV-hsyn-Jaws-KGC-GFP-ER2 Optogenetics... AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-9-PV2527 99039-AAV9 pAAV-CamKII-ArchT-GFP (PV2527) Optogenetics...29777-AAV5 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV3446 59170...59170-AAV9 pAAV-Syn-Chronos-GFP Ed Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40 Loren Looger...Ed Boyden AV-1-PV3446 59170-AAV1 pAAV-Syn-Chronos-GFP Optogenetics Ed Boyden AV-1-PV3447 59171-AAV1 pAAV-Syn-ChrimsonR-tdT...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... PINK1 N-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's...21190 GFP-pcDNA3-PKCgamma-cys1Acys1B PRKCG GFP CMV Spinocerebellar ataxia 27 Tobias Meyer 21204 GFP-N2-PKCgamma...PKCgamma PRKCG GFP CMV Spinocerebellar ataxia 26 Tobias Meyer 21205 GFP-C1-PKCgamma-C1A PRKCG GFP CMV Spinocerebellar...Parkinson's, FTD Michael Davidson 57146 mPA-GFP-MAPTau-N-10 MAPT GFP CMV Parkinson's, FTD Michael Davidson...-HtrA2-EGFP HTRA2 GFP CMV Parkinson's L. Miguel Martins 14122 pcDNA3-HtrA2-EGFP S306A HTRA2 GFP CMV Parkinson's... GPD 25QDProGFP p416 HTT GFP GPD Huntington's Susan Lindquist 15569 GPD 104QDProGFP p416 HTT GFP GPD Huntington's...GAL 25Q+ProGFPp416 HTT GFP GAL1 Huntington's Susan Lindquist 15581 GAL 46Q+ProGFPp416 HTT GFP GAL1 Huntington's...

  7. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...68299 GFP E17TAG/Q157TAG Plasmid 68298 GFP S2TAG/E17TAG Plasmid 68297 GFP Q157TAG Plasmid 68296 GFP S2TAG...Library 111704 Mode #1 Library Plasmid 69118 E17TAG GFP zeo resistance Plasmid 68307 SupD Strain 68306 C321...S2TAG Plasmid 68295 GFP E17TAG Plasmid 68294 SepOTSν Plasmid 68292 SepOTSλ Plasmid 68291 SepOTSκ Plasmid...pSerOTS-C1* (V70) is a ROP minus plasmid and is not compatible with other plasmids containing high copy ColE1...

  8. Viral Production

    Type
    Collection
    ... later, Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the...viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, ... prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...

  9. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP . Technology...internal or surface proteins Single constructs are compatible with multiple applications (different fluorophores...

  10. Serotype Testing AAV

    Type
    Collection
    ...AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV in...plasmid 37825 (deposited by Edward Boyden ) and direct GFP expression from the CAG promoter. For information... available from Addgene's viral service. Control EGFP vectors in various serotypes for serotype testing...encode fluorescent reporters and can be used to compare the tropism of different serotypes. In addition... 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP (Plasmid #50465) Description : Ready-to-use AAV ...plasmid 50465 (deposited by Bryan Roth ) and direct EGFP expression from the human synpasin promoter. For...

  11. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ...2020 GENIE Project Calcium jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing... FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP... vascular smooth muscle cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008...autophagic flux by pH-sensitive fluorescence (pMRX-IP-GFP-LC3-RFP) An Autophagic Flux Probe that Releases an...

  12. Bacterial Expression Systems

    Type
    Collection
    ...Vladislav Verkhusha 52732 52733 pET11a-link-NGFP pMRBAD-link-CGFP GFP (reconstructed) BiFC Lynne Regan 168257...Fluorescence (GFP+) Gram-negative bacteria Philip Poole 14460 pOT2 Promoter activity Fluorescence (GFPuv) Gram-negative... Guide to Selecting Fluorescent Dyes and Ligands GFP Fusion Proteins — Making the Right Connection Photoactivatable...pRsetB-his7-Perceval ATP:ADP ratio Fluorescence (GFP) Escherichia coli Gary Yellen 187836 pVoPo-01 Promoter...bacteria Philip Poole 14083 pAKgfplux1 Promoter activity Fluorescence (GFPmut3a) and luminescence (lux operon...bacteria Attila Karsi 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a) Gram-negative bacteria Attila...-M ATP Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC Cyclic di-GMP Fluorescence...

  13. Tetracycline Inducible Expression

    Type
    Collection
    ...Iwasato 63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or...transactivators and promoters are generally cross-compatible. Your choice of transactivator, promoter, and...et al., 2016 (Link opens in a new window) for comparison of Tet-On systems in different applications. ... Off) None TRE, miniCMV Maria Lung 16542 pBI-MCS-EGFP Bidirectional promoter (Pbi) for Tet-responsive ...

  14. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...marker like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with fluorescence...detecting multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence...

  15. Antibody Production

    Type
    Collection
    ... not endogenously-expressed (e.g., using an anti-GFP antibody), the target antigen is first transiently...containing 1 mM sodium azide. This buffer is not compatible for use in live cells and will interfere with...secondary antibody. The fluorescence pattern is compared to an untransfected control. Immunohistochemistry...secondary antibody. The tissue labeling pattern is compared to the mRNA expression pattern cited in the Tissue...HRP-conjugated secondary antibody. Protein expression is compared to an untransfected control and the protein size...

  16. Antibody Plasmid Collection

    Type
    Collection
    ...find antibody plasmids for: Common antigens such as GFP or mCherry Monoclonals, Nanobodies, Sybodies, or ...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their... and Evolving Functional Proteins. METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995). Carlos...

  17. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...157970 pTR-UF11 chimeric CMV/Chicken Beta actin (CBA) GFP Control Sergei Zolotukhin Citation Information AAV2...intravitreal injection in mice and marmosets as compared to the AAV2 serotype. The AAV2(Y444F) serotype...in the retina following subretinal injection as compared to the parental AAV44.9 serotype. It was developed...acknowledge Shannon Boye and cite Boye, et al. 2016. Impact of Heparan Sulfate Binding on Transduction of Retina...

  18. Biosensor AAV Preps

    Type
    Collection
    ...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Edward Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn...Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1...Podgorski Calcium Sensor: CaMPARI 100831 pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40 CAG CaMPARI none Cre dependent ...dependent 5 Loren Looger 100832 pAAV.hSyn.CaMPARI.WPRE.SV40 Syn CaMPARI none Constitutive 1, 5, 9 Loren Looger...Looger 101060 pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive 1 Eric Schreiter 101061 pAAV_hsyn_NES-his-CaMPARI2... pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 Syn CaMPARI2-F391W his Constitutive 1 Eric Schreiter 101064...101064 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 Syn CaMPARI2-L398T his Constitutive 1 Eric Schreiter Calcium...

  19. Neurodegeneration Research Collection

    Type
    Collection
    ... Use the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP, or IP with Flag...Collection . Express EGFP-tagged wt and mt tau . (Hoover et al. Neuron. 2010) Express EGFP-tagged wt and mt... to using plasmid pooled libraries . Use the ultracompact and programmable endonuclease, TnpBmax , for...lentiviral expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer et al. Nat Neurosci. 2019) pLVX-UbC-rtTA-Ngn2...

  20. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and... exchange (RMCE): When matching but mutually incompatible recognition sites flank both a DNA target site...
Showing: 1 - 20 of 24 results