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Showing: 1 - 20 of 58 results
  1. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    .../Membrane, Excitation: 400 / 504, Emission: 515 / 517mPA-GFP-CD151-7 Data Supplement mPA-GFP-C-Src-...: 400 / 504, Emission: 515 / 517mPA-GFP-EB3-7 Data Supplement mPA-GFP-ER-3Localization: Endoplasmic...: 400 / 504, Emission: 515 / 517mPA-GFP-MannII-N-10 Data Supplement mPA-GFP-MAP4-C-10Localization:...mPA-GFP-MCHR1-N-10Localization: Cilium, Excitation: 400 / 504, Emission: 515 / 517mPA-GFP-MCHR1-N-.../ 504, Emission: 515 / 517mPA-GFP-MyosinIIA-C-18 Data Supplement mPA-GFP-Paxillin-22Localization:...517mPA-GFP-Rab4a-7 Data Supplement mPA-GFP-SiT-N-15Localization: Golgi TGN, Excitation: 400 / 504,...517mPA-GFP-Mito-7 Data Supplement mPA-GFP-MyosinIIA-C-18Localization: Cytoskeleton, Excitation: 400.../ 517mPA-GFP-Dectin1A-C-10 Data Supplement mPA-GFP-Dectin1A-N-10Localization: Membrane, Excitation...: 400 / 504, Emission: 515 / 517mPA-GFP-Dectin1A-N-10 Data Supplement mPA-GFP-EB3-7Localization: MT...517mPA-GFP-Farnesyl-5 Data Supplement mPA-GFP-Fibrillarin-7Localization: Nucleoli, Excitation: 400...mPA-GFP-MAP4-C-10Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517mPA-GFP-MAP4-C...: 515 / 517mPA-GFP-Rab4a-7 Data Supplement mPA-GFP-SiT-N-15Localization: Golgi TGN, Excitation: 400.../ 504, Emission: 515 / 517mPA-GFP-SiT-N-15 Data Supplement mPA-GFP-Talin-ABM-10Localization: Talin.../ 517mPA-GFP-TOMM20-N-10 Data Supplement mPA-GFP-Tubulin-6Localization: Microtubules, Excitation:...
  2. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression mPA-GFP-N1 - Mammalian Expression mPA-GFP-C1...487 509 - Monomer (A206K) mPA-Emerald-N1 - Mammalian Expression mPA-Emerald-C1 - Mammalian Expression...then scientists have engineered numerous GFP-variants and non-GFP proteins that result in a diverse...pRSETA-PAGFP - Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast...Prone to dimerization PA-sfGFP-N1 - Mammalian Expression PA-sfGFP-C1 - Mammalian Expression mPA-Emerald...The original green fluorescent protein (GFP) was cloned in 1992 (Prasher et al., Gene, 1992), and since...Proteins that are derived from GFP and contain the A206K mutation are monomeric at all concentrations...- Yeast Expression pFA6a-link-yEPAGFP-CaUra3 - Yeast Expression mPA-GFP-N1 - Mammalian Expression mPA-GFP-C1...mPA-Emerald 487 509 - Monomer (A206K) mPA-Emerald-N1 - Mammalian Expression mPA-Emerald-C1 -...530 541 36 6.8 Monomer mPapaya1-N1 - Mammalian Expression mPapaya1-C1 - Mammalian Expression...- Bacterial Expression ZsYellow1 529 539 8 Tetramer ZsYellow1-N1 - Mammalian Expression mPapaya1...sfGFP 485 507 54 Prone to dimerization sfGFP-N1 - Mammalian Expression sfGFP-C1 - Mammalian...mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - Bacterial Expression...Expression sfGFP-pBAD - Bacterial Expression moxGFP 486 510 50 Monomer (A206K) moxGFP - Mammalian...
  3. Cell Migration Consortium Plasmids

    Type
    Collection
    ...Expression, RetroviralpBabe-TetCMV-puro Hahn 22032pBabe-TetCMV-puro-mPA-GFP-PA-Rac1mPA-GFP-PA-Rac1...(Homo sapiens)Mammalian Expression, Bacterial Expression, Insect ExpressionpTriEx6X His, mPA-GFP...Bacterial Expression, Insect ExpressionpTriEx6X His, mVenus Hahn 22022pTriEx-mPA-GFP-PA-Rac1PA-Rac1...sapiens)Mammalian Expression, RetroviralpMX-uGFPGFP Brugge 14567pMX GFP...Homo sapiens)Mammalian Expression, RetroviralpMXEGFP Brugge 14568pMX GFP...(Caenorhabditis elegans)Bacterial ExpressionpET17His Vale 24472U371-GFP...(Caenorhabditis elegans)Bacterial ExpressionpET17GFP Vale 24473U653-GFP...Expression, RetroviralpBabe-TetCMV-puro Hahn 22032pBabe-TetCMV-puro-mPA-GFP-PA-Rac1mPA-GFP-PA-Rac1...ExpressionpcDNA3EGFP Hahn 12601pcDNA3-EGFP-Cdc42(T17N)EGFP-Cdc42 (T17N...Hahn 12599pcDNA3-EGFP-Cdc42(wt)EGFP-Cdc42 (WT) (Homo sapiens)Mammalian ExpressionpcDNA3EGFP...13721pcDNA3-EGFP-Rac1(T17N)EGFP-Rac1(T17N) (Homo sapiens)Mammalian ExpressionpcDNA3EGFP...Expression, RetroviralpMX-uGFPGFP Brugge 14567pMX GFP Rac G12VRac1 G12V...
  4. Plasmids for Optogenetics Research

    Type
    Collection
    ...LOV Retroviral mPA-GFP Hahn 22034 pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M LOV Retroviral mVenus Hahn...22031 pBabe-TetCMV-puro-mCerulean-PA-Rac1 LOV Retroviral mCerulean Hahn 22032 pBabe-TetCMV-puro-mPA-GFP-PA-Rac1...Retroviral mVenus Hahn 22021 pTriEx-mVenus-PA-Rac1-C450A LOV Multiple mVenus Hahn 22022 pTriEx-mPA-GFP-PA-Rac1...  Inhibitory Arch AAV GFP Boyden 22223 FCK-Mac-GFP Inhibitory Mac Lentiviral GFP Boyden 22224 FCK-ss-Prl-Arch-GFP...CsChR AAV GFP Boyden 58849 pAAV-CaMKII-Mac-GFP Inhibitory Mac AAV GFP Boyden 58851 pAAV-EF1a-FLEX-ArchT-GFP...GFP Boyden 59056 pCAG-FLEX-rc[Chronos-GFP] Excitatory Chronos Mammalian GFP Boyden 59070 pAAV-Syn-CoChR-GFP...Inhibitory ArchT AAV GFP Boyden 29777 pAAV-CAG-ArchT-GFP  Inhibitory ArchT AAV GFP Boyden 29778...AAV GFP Boyden 58852 pAAV-Syn-FLEX-Mac-GFP Inhibitory Mac AAV GFP Boyden 58853 pAAV-Syn-Mac-GFP...CIBN-GFP-TMD Cry2-CIB1 Mammalian EGFP De_Camilli 79572 Lyn11-CIBN-GFP Cry2-CIB1 Mammalian EGFP De_Camilli...Mac AAV GFP Boyden 58805 pAAV-CaMKII-Chronos-GFP Excitatory Chronos AAV GFP Boyden 58806 pAAV-Syn-ChrimsonR-GFP...
  5. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...-CatCh-GFP-WPRECatCh-GFP (Synthetic)AAVpAAV Roska 9_pAAV-ProC5-CatCh-GFP-WPRECatCh-GFP...-CatCh-GFP-WPRECatCh-GFP (Synthetic)AAVpAAV Roska IV_pAAV-ProB6-CatCh-GFP-WPRECatCh-GFP...-CatCh-GFP-WPRECatCh-GFP (Synthetic)AAVpAAV Roska 60_pAAV-ProA17-CatCh-GFP-WPRECatCh-GFP...-CatCh-GFP-WPRECatCh-GFP (Synthetic)AAVpAAV Roska 76_pAAV-ProA25-CatCh-GFP-WPRECatCh-GFP...-CatCh-GFP-WPRECatCh-GFP (Synthetic)AAVpAAV Roska 83_pAAV-ProC14-CatCh-GFP-WPRECatCh-GFP...-CatCh-GFP-WPRECatCh-GFP (Synthetic)AAVpAAV Roska 198_pAAV-ProD1-CatCh-GFP-WPRECatCh-GFP...-CatCh-GFP-WPRECatCh-GFP (Synthetic)AAVpAAV Roska 146_pAAV-ProA34-CatCh-GFP-WPRECatCh-GFP...Schreiter pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40CaMPARI2_F391W-G395D (Synthetic...-F391W-L398VCaMPARI2 (Synthetic), CaMPARI2 (Synthetic)Mammalian Expression, AAVpAAVNES-his...(Synthetic)Mammalian Expression, AAVpAAV Looger pAAV.hSyn.CaMPARI.WPRE.SV40CaMPARI...pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40CaMPARI2-H396K (Synthetic)AAVpAAVNES_his, FLAG-HA-myc...)AAVpAAVNES_his, FLAG-HA-myc Schreiter pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40CaMPARI2...-WPRE-SV40CaMPARI2 (Synthetic)AAVpAAVNES_his, FLAG-HA-myc Schreiter pAAV_hsyn_NES-his-CaMPARI2...AAVpAAV-EF1α1.1tdTomato (codon diversified version) Boyden pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3rsCaMPARI...
  6. Zebrafish Plasmid Collection

    Type
    Collection
    ...Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.Cas9-T2A-GFP (Danio rerio)...Ahituv 37846E1b-GFP-Tol2-Gateway Ahituv...driven by a UAS promoter5x UAS (Saccharomyces cerevisiae), Cas9 (Other), T2A, GFP, U6 promoter (Danio...5UAS DSRedExpressEncodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for...from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP...sapiens) Guler 7431414UAS zfPSD95:GFPA fusion of zebrafish PSD95 to GFP..., 5xU6:sgRNAExpression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT...expression of rsCaMPARI in zebrafish, nucleus excludedrsCaMPARI (Synthetic) Schreiter...expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)HuC...Zebrafish Tol2 Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab....A genetic binary system for cell- and compartment-specific biotin labeling in zebrafish. pGCS Vector...(elavl3)-CaMPARI2-FLAG-HA-myc-polyA (Rattus norvegicus) Schreiter 154911pCS2...gfap promotergfap promoter (Danio rerio) Eisen 82402p5E-mnx1Gateway-compatible...elavl3 promoterelavl3 promoter (Danio rerio) Eisen 82401p5E-gfapGateway-compatible...
  7. Synthetic Biology - Bacterial

    Type
    Collection
    ...pHL1853PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA GFP (Other), tetR...pHL1855PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA GFP (Other), tetR...pHL1529PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA GFP (Other), tetR, csrA...pHL1757PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR GFP (Other), tetR (Other)Bacterial Expression...pHL 246AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1 GFP (Other)Bacterial Expression...pHL1914PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR GFP (Other), tetR (Other)Bacterial Expression...pHL 1073pCon:ompC::mCherry, pLlacO-1:sodB::gfp mCherry (Other), GFP (Other)Bacterial Expression, Synthetic...pCDFBB-eGFPegfpSynthetic Biology Schmidt-dannert Optimized compatible set...pACBB-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set...pUCBB-pBAD-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...pUCBB-ptet-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...pUCBB-ctH6-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...BsaI, BbsI compatible deGFP Murray Linear DNA for rapid prototyping of...pJexpress414_OmpA171_WTWild-type OmpA (N-terminal domain) with an HA tag in the 4th external loopOmpA...
  8. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...PP295Expresses GFP. Figure 3....PP296Expresses GFP. Figure 3....PP297Expresses GFP. Figure 3....PP298Expresses GFP. Figure 3....PP299Expresses GFP. Figure 3....PP300Expresses GFP. Figure 3....PP318Expresses GFP. Figure 3....pCDFBB-eGFPegfpSynthetic Biology Schmidt-dannert Optimized compatible set...pACBB-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set...pUCBB-pBAD-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...pUCBB-ptet-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...pUCBB-ctH6-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...pJexpress414_OmpA171_WTWild-type OmpA (N-terminal domain) with an HA tag in the 4th external loopOmpA...pJexpress414_OmpA171_H346OmpA redesign hybrid with wild-type strands 346OmpA_H346 (Synthetic)Bacterial...
  9. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...pCXLE-dCas9VPH-T2A-GFP-shP53dCas9VPH-T2A-GFP-shP53...pCXLE-dCas9VPPH-T2A-GFP-shP53dCas9VPPH-T2A-GFP-shP53...Cas9 GFP plasmid constitutively expressed from CAG promoter....col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression....EBNA episome plasmid for CAG promoter constitutive expression of dCas9-GFP....Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif....impaired BE3 mutant)....For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat....For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat....For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat....
  10. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...pCXLE-dCas9VPH-T2A-GFP-shP53dCas9VPH-T2A-GFP-shP53...pCXLE-dCas9VPPH-T2A-GFP-shP53dCas9VPPH-T2A-GFP-shP53...-p65-HSF1-P300-T2A-GFP-IRES-Neo (Other)TRE-tightNeomycin (select with G418) Otonkoski...-T2A-GFP-shP53 (Other)CAG Otonkoski Human pluripotent reprogramming with...-p65-HSF1-T2A-GFP-IRES-Neo (Other)CAGNeomycin (select with G418) Otonkoski...-P300-T2A-GFP-IRES-Neo (Other)CAGNeomycin (select with G418) Otonkoski Human...Co-expresses human optimized S. pyogenes dCas9-VP64 and GFP FUGW...Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter lentiCRISPR v2...pAct:dCas9-VP64dCas9-VP64pActin (Drosophila) Perrimon Comparison of Cas9...pAct:dCas9-GCN4dCas9-10XGCN4pActin (Drosophila) Perrimon Comparison of Cas9...Lentivirus compatible dCas9-VPR construct driven by the CAG promoter lentiCRISPR v2...pAct:dCas9-VPRdCas9-VPRpActin (Drosophila) Perrimon Comparison of Cas9 activators...Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter lentiCRISPR v2...-VPRdxCas(3.7)-VPR (Synthetic) Liu Evolved Cas9 variants with broad PAM compatibility...
  11. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...65014-AAV5 pAAV-hsyn-Jaws-KGC-GFP-ER2 Ed Boyden AV-8-PV3638 65014-AAV8 pAAV-hsyn-Jaws-KGC-GFP-ER2...Ed Boyden AV-1-PV2509 29777-AAV1 pAAV-CAG-ArchT-GFP Ed Boyden AV-1-PV2527 99039-AAV1 pAAV-CamKII-ArchT-GFP...Ed Boyden AV-9-PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV2527 99039-AAV9 pAAV-CamKII-ArchT-GFP...(PV2527) Ed Boyden AV-1-PV3446 59170-AAV1 pAAV-Syn-Chronos-GFP Ed Boyden AV-1-PV3447 59171-AAV1...Ed Boyden AV-5-PV2527 99039-AAV5 pAAV-CamKII-ArchT-GFP (PV2527) Ed Boyden AV-5-PV3446 59170-AAV5...(PV2527) Ed Boyden AV-9-PV3446 59170-AAV9 pAAV-Syn-Chronos-GFP Ed Boyden AV-9-PV3447 59171-AAV9...pAAV-Syn-Chronos-GFP Ed Boyden AV-5-PV3447 59171-AAV5 pAAV-Syn-ChrimsonR-tdT Ed Boyden AV-5-PV3638...Wilson AV-1-PV3690 100832-AAV1 pAAV.hSyn.CaMPARI.WPRE.SV40 Loren Looger, Eric Schreiter AV-1-PV3844...AV-1-PV4705 101061-AAV1 pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40 Eric Schreiter AV-5-52925 52925...Wilson AV-5-PV3690 100832-AAV5 pAAV.hSyn.CaMPARI.WPRE.SV40 Loren Looger, Eric Schreiter AV-5-PV4090...100831-AAV5 pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40 Loren Looger, Eric Schreiter AV-5-PV4618 100889-AAV5...Wilson AV-9-PV3690 100832-AAV9 pAAV.hSyn.CaMPARI.WPRE.SV40 Loren Looger, Eric Schreiter AV-9-PV3849...AAV1 pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 GENIE, Douglas Kim AV-1-PV4703 101060-AAV1 pAAV_hsyn_NES-his-CaMPARI2...-WPRE-SV40 Eric Schreiter AV-1-PV4704 101064-AAV1 pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40 Eric Schreiter...
  12. Antibodies

    Type
    Collection
    ...(MoonTag-Nb-GFP)Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells....CRY2 PHR E490G fused to anti-GFP nanobody for optogeneticsCRY2 PHRolig-VHH(GFP) (Arabidopsis thaliana...)express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogeneticsCRY2 PHR-VHH(GFP) (Arabidopsis...nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins....nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins....METHODS: A Companion to Methods in Enzymology 8, 94–103 (1995)....access to a large number of standardized renewable affinity reagents (to cancer-associated targets) and accompanying...(MoonTag-Nb-GFP)Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells....of vsfGFP-9....anti-GFP Nanobody in E. coli cells.GFP-Nanobody (Synthetic) Nakayama...ferritinExpresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin...of GFP nanobody-Fc antibody domain fusionHuman IgG1 Fc-domain fused with GFP nanobody (Homo sapiens)...
  13. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...expression of FnCas9-GFP in bacterial cells pET-His6-GFP-TEV-LIC...Cas9 GFP plasmid constitutively expressed from CAG promoter....col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression....Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone....Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker....Ubiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif....Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif....Compatible with MultiSite Gateway cloning N/A...For use in plants and compatible with the MultiSite Gateway system pDONR-221z...Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible...Nme2Cas9_pCDestNme2Cas9 (Other)CMV Sontheimer A Compact...It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence...
  14. Synthetic Biology - Sensing and Signaling

    Type
    Collection
    ...pAWp7-10UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively...pAWp7-12UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-124]Lentiviral Lu Massively...pAWp7-13UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively...pAWp7-15UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-128]Lentiviral Lu Massively...pAWp7-16UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively...pAWp7-18UBCp-RFP-[miR-132 sensor]-CMVp-GFP-[miR-132]Lentiviral Lu Massively...pAWp7-5UBCp-RFP-CMVp-GFP-[miR-128 + miR-132]Lentiviral Lu Massively parallel...pTD103luxI_sfGFPLuxI, sfGFP, and LuxRBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA...pZE-PalkB-GFPGFP, PalkBBacterial Expression, Synthetic BiologyPalkB, PalkB Alper...pPhlF-BCD22-sfGFP-ssrA P64U26C39T11 v1-1 (632)sfGFP-ssrA Murray Rapid cell-free...pSCKtheoRaj12-olsaTheoHHAzRaj12,cisRaj12,sfGFP (Synthetic), theoHHAzRaj12,cisRaj12-sfGFP (Synthetic)Bacterial...pTD103luxI_sfGFP -RsfGFP and luxIBacterial Expression, Synthetic BiologypLuxssrA tag (AANDENYALAA)...pJexpress414_OmpA171_H1346OmpA_H1346 (Synthetic)Bacterial Expression, Synthetic BiologyT7FlAsH, 6His...pJexpress414_OmpA171_H1256OmpA_H1256 (Synthetic)Bacterial Expression, Synthetic BiologyT7FlAsH, 6His...
  15. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...pAWp7-14pFUGW-UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128]UBCp-RFP-[miR-128 sensor]-CMVp-GFP-[miR-128...pAWp7-2pFUGW-UBCp-RFP-CMVp-GFP-[miR-128]UBCp-RFP-CMVp-GFP-[miR-128]Lentiviral Lu...E0040m (GFP)_CDMoClo Basic Part: CDS - Fluorescent protein. Green....pJWV25To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via...pHL391Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry...pHL417Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::micC::Asp terminator +...pHL836Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator +...pCDFBB-eGFPegfpSynthetic Biology Schmidt-dannert Optimized compatible set...pACBB-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible set...pUCBB-pBAD-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...pUCBB-ptet-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...pUCBB-ctH6-eGFPeGFPSynthetic Biology Schmidt-dannert Optimized compatible...Expression, Synthetic Biology Schmidt-dannert Optimized compatible set of...compatible set of BioBrick vectors for metabolic pathway engineering....
  16. Bacterial Expression Systems

    Type
    Collection
    ...contains GFP but all others are empty....araBAD promoter whereas heterodimer formation with dominant negative AraC decreases GFP expression....Can be used in combination with a fluorophore-tagged MS2 (such as that found in pZS*12-MS2-GFP) to track...pAK Series Various Plasmids pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range vectors...pET LIC Series Various Plasmids T7-lacO (lactose/IPTG inducible) Flag, His, Strep, mOCR, MBP, GFP, with...Preferential association of AraC-fusion homodimers results in the activation of GFP expression from the...Compatible with Gateway cloning..... pPro24-gfp contains GFP but all others are empty....Comprehensive multi-cloning site compatible with standard cloning using many different restriction enzymes...
  17. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Early endosomes Rab5B AcGFP Voeltz GFP-Rab4B Early endosomes Rab4B AcGFP Voeltz GFP-rab7 WT Late...Rab7a mCherry Voeltz GFP-Rab7A Late endosomes Rab7a AcGFP Voeltz GFP-rab11 WT Recycling endosomes...Adherens junctions E-cadherin GFP Stow Paxillin-pEGFP Focal adhesions paxillin GFP Horwitz pPaxillin-PSmOrange...Mitochondria ABCb10 GFP Shirihai mCherry-Mito-7 Mitochondria Mitochondrialtargeting sequence(COX8A...Targeting Gene/Signal Fluorescent Protein PI H2B-mCherry Chromatin H2B mCherry Benezra H2B-GFP...For example, if the marker protein is fused to GFP, you may want to fuse your protein to a red fluorescent...Microtubules EB1 GFP Shaw pmiRFP703-Tubulin Microtubules alpha-tubulin miRFP703 Verkhusha pEB3...One of the most common is to use fluorescence microscopy to compare the localization of your protein...-N Extracellular milieu cfSGFP2-N EGFP Wada Lck-GFP Membrane palmitoylation sequence from Lck EGFP...Early endosomes Rab5B AcGFP Voeltz GFP-Rab4B Early endosomes Rab4B AcGFP Voeltz GFP-rab7 WT Late...Microtubules EB1 EGFP Takemaru pEGFP-rNFM Intermediate Filaments (neuronal) Nefm EGFP Brown GFP-hChibby1...Base Chibby1 EGFP Takemaru GFP-hCCDC11 Centriolar satellites CCDC11 EGFP Takemaru *Fusions to...utrophin (aa# 1-261) GFP Bement pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin EGFP Wadsworth...Rab7a mCherry Voeltz GFP-Rab7A Late endosomes Rab7a AcGFP Voeltz GFP-rab11 WT Recycling endosomes...
  18. Cre-lox system

    Type
    Collection
    ...Cre and GFP coexpression CAG Retroviral Gage 49054 CAG-GFP/cre Cre-GFP fusion CAG Retroviral Gage...GFP and Cre CAG Mammalian Lu 117148 Hiv7CMV-Cremyc-2A-GFP GFP and Cre CMV Lentiviral Lu 118029...Bacterial Liu 63704 pRetroX GFP T2A Cre GFP and Cre tight TRE Retroviral Foijer 64769 pTW040 Cre...49056 AAV-GFP/Cre Cre-GFP fusion CMV AAV Gage 49111 pEMS1980 iCre with MCS for inserting promoter...on GFP (CRE-DOG) CAG Mammalian Cepko 69573 pCAG-C-CreintG Cre recombinase dependent on GFP (CRE-DOG...iRFP670, Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler 68552 HRE:GFP-pGK...fusion Rous sarcoma virus (RSV) LTR/promoter Mammalian Sauer 11920 pBS500 EF1alpha-GFPcre Cre-GFP...constructs also contain fluorescent labels that indicate if recombination has occurred, allowing for direct comparison...Cre-GFP fusion EF-1 alpha Mammalian Sauer 11923 pBS598 EF1alpha-EGFPcre Cre-EGFP fusion EF-1 alpha...tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre..., Cre, and GFP TRE Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler 68552 HRE:GFP-pGK:Cre...Cre dependent GFP expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch plasmid...
  19. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Please note- this plasmid does not express GFP....Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker....Cas9 inducible by removal of a GFP flip-out cassette pUASTattB...(M)Cas9FRT-GFP-FRT-uORF(M)Cas9 Boutros A large-scale resource for tissue-specific...pCRISPaint-T2A-Cas9-T2A-GFP...-3xP3-RFPCas9-T2A-GFP Perrimon Gene Knock-Ins in Drosophila Using Homology-Independent...UAS-FRT-GFP-FRT-u...pAct:dCas9-VPRdCas9-VPRpActin (Drosophila) Perrimon Comparison...pAct:dCas9-VP64dCas9-VP64pActin (Drosophila) Perrimon Comparison...pAct:dCas9-GCN4dCas9-10XGCN4pActin (Drosophila) Perrimon Comparison...Tribolium basal heat shock promoter driving Cas9 pSLfa[Tc-bhsp68::nlsEGFP]fa...
  20. Worm Expression Resources

    Type
    Collection
    ...-mex3Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjectionPmex-5:GFP::H2B::PEST...+ SEC)SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology armsPmyo-2::GFP + SEC (Caenorhabditis...34877pCFJ420 - Peft-3::GFP::H2B (ubiquitous)Peft-3 GFP H2B tbb-2 UTR (Caenorhabditis elegans)...A set of vectors for C. elegans research, including lacZ and GFP fusion vectors....(dpiRNA)::CDK-1cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode....elegans) Kuwabara 38147pPK720dpy-7p::ptc-3::gfp Kuwabara...Backbone pPD95.79 (Firelab).firefly luciferase luc+ fused to GFP (S65C) (Other) Glover...endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible...(myo-3::sfGFP::let-858, using backbone as in L3785)superfolder GFP (Synthetic) Fire...75027pJJR82EGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsEGFP^SEC^...GFP is optimized by removing all piRNA targeting sites to allow germline expression.GFP(dpiRNA)::CDK-...Vellai 116943pSM-GFPnovo2GFPnovo2_unc-54 utr vector for expression in C. elegansC. elegans...-GFP (Caenorhabditis elegans) Sternberg 85818pCS2+ cMyc-oDam_f_GFPoNLSiDamID...
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