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We narrowed to 474 results for: mag

Showing: 141 - 160 of 474 results

  1. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...for certain applications such as in vivo tissue imaging.   Figure 1: Ace-mScarlet is a fusion ... a proportion of mScarlet's emission via FRET.  Image from Beck et al, 2019 under CC BY 4.0.   FRET...This system is particularly powerful for in vivo imaging especially since JFs can cross the blood brain ...targeting your cell type of interest. For in vivo imaging applications, the ability to direct your voltage...

  2. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... antigens as measured by suspension bead assay. Image from  Preger et al., 2020. Preger et al., Journal...3: Schematic representation of the MPyV system. Image from Cheah et al., 2021.   After validating ... vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2... terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded...

  3. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...engineering of biology much easier. So you could imagine engineering the entirety of a cell. That's a big... or so sensor domains for everything you could imagine. That I think is the next priority; we need the...clear, but the way I'd leave it is mostly to the imagination. The question is: what can you use biology to...off-the-shelf genetic elements that allow you to begin to imagine designing 10-gene systems with quantitative precision...

  4. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should...enough information to tell a compelling story in an image or do you need a full exhibit? In a five minute ... to convey an entire story or context via still images. Some things cannot be photographed or need text...interpretation. Infographics Low Combines both words and images; especially useful for conveying and contextualizing...

  5. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously   Researchers...energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate the power...shown great performance in cellular functional imaging and in protein fusions. The Gadella lab has deposited...toolbox of fluorescent proteins (FP) for cellular imaging is constantly expanding. Case in point - check ...could be better suited for deep-tissue in vivo imaging. These variants were developed as part of a larger...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al., 2018. APOBEC-Cas9n-UGI...Editing efficiencies using base editing reporters. Image from St. Martin et al., 2019. The Harris lab ...results in shift in emission spectra from BFP to GFP. Image from Standage-Beier et al., 2019. The team put...luciferase reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of...

  7. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...Central PMCID: PMC4364232. 2. Baker, Monya. “RNA imaging in situ”. Nature Methods 9, 787–790 (2012). 3. .... Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer...PMCID: PMC4071757. 6. Hong, He, et al. “Molecular Imaging with Nucleic Acid Aptamers.” Curr Med Chem.  2011...

  8. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...non-fluorescent, and misfolded FP-fusions on quantitative imaging experiments and cell physiology in general. Pitfalls...readily apparent and can confound quantitative imaging experiments or even negatively impact cells (see... represent one of the most inert solutions for imaging in a variety of cellular compartments including...proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also an avid long distance ...

  9. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...material is a factor of practicality (given image sizes) but images should be unprocessed and in capture format...other than the actual author. Instead, selected images are said to be representative or the statistical...charts and tables in the paper are in scope. For images, the number of examples supporting the published...

  10. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...blocking, and washing, the membrane is reassembled and imaged. In this example, a 1:1,000 dilution gives a high...eye; a fluorescent ladder compatible with your imaging system; or a combination of the two. There are ... wash for the full amount of time, every time. Imaging The last step in a western blot tends to be the...technical design of a western blot”) post, most imaging issues can be fixed real-time by exposing for either...seeing high background or low signal during the imaging process, try adjusting your exposure time first...

  11. Antibody Guide

    Type
    Guide
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...a substrate kit. Image the membrane using either X-ray film in a darkroom or an image capture machine....reaction can be detected either digitally by an imaging machine or by X-ray film. HRP cannot be used to...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...antibody, incubate, and wash. Preserve sample and image using a microscope. Special considerations Many ...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...Scientists around the world began looking for the magic molecule that serves as the code of life. In 1944...Raymond Gosling took the first ever X-ray diffracted image of the DNA. Finally, in 1953, Franklin along with...the two most popular topics that sparked the imagination of most of the authors during this period.  Genetic...engineering in pop culture SciFi is one of the most imaginative and most important genres of literary fiction...

  13. CRISPR Guide

    Type
    Guide
    ...repress target genes, purify specific regions of DNA, image DNA in live cells, and precisely edit DNA and RNA...antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9-VPR...Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including the simplicity...detect multiple targets simultaneously. CRISPR imaging can visualize chromatin and RNA dynamics in live...RNA-binding proteins (RBPs). Multicolor CRISPR imaging can be achieved by using orthogonal dCas9s (e.g... stability and amplifies the signal for better imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent...Cas13 fusions have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: ...

  14. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...of regenerative medicine is to replace lost or damaged cells. Here, I will discuss the two major processes... is observed in the pancreas when excess β-cell damage results in the transdifferentiation of glucagon-producing...cells from the patient in vitro then correct the damaged gene in the resulting iPSCs before differentiating...to replenish and repair cells that are lost or damaged. Both transdifferentiation and reprogramming give...

  15. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen...Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's...

  16. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ... beautiful Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids... plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create...Preferences” and click on the “Filters & Icons” link (see image to the right). Once on the “Filters” page, select...

  17. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... them. For the application we were looking at, magnetic isolation of circulating tumor cells, we found...may have an impact on this challenge either for magnetic capture or for capture using microfluidic devices...peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad...

  18. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    ...through entire papers, perhaps you search google images for specific experimental results but, obviously...simple Chrome plugin that allows you to save any image from articles openly available on PubMed Central.... For example, you might include a confirmatory image to show that a previously used reagent performs ...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...control. C) Iterative transduction workflow overview. Image from Sun et al. 2021. The Organoid EasyTag ...of the receptor-based SL fluorescent biosensor. Image from Chesterfield et al., 2020     Chesterfield... calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128...

  20. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    ...retain the stop cassette following recombination. Image from Isaacman-Beck et al., 2019. Sparse Predictive...indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck et al., 2019. Applications...for optogenetics. The Clandinin lab used SPARC to image the calcium response in the dendrites of T5 neurons...
Showing: 141 - 160 of 474 results