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We narrowed to 560 results for: mme

Showing: 141 - 160 of 560 results

  1. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...optimization. Before beginning an experiment, it is recommended that you try a few different ratios of transfer...will need to be optimized prior to starting. We recommend calculating the ratio of the surface area of the... target cell line. To overcome this issue, we recommend using a range of doses of lentivirus when transducing...

  2. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...scientists, I have spent hours sifting through comments and papers to find the most reliable (and cost-effective... crisis” came to light as antibodies became commercialized, revealing the unreliability of antibodies....antibody crisis states that up to 50% of all commercially available antibodies are unreliable (Baker, ...

  3. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior to their...of cell culture medium on the packaging cells immediately following transfection. In other words, when ...

  4. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...trained our models in close collaboration with the Krauthammer lab (University of Zurich), especially with my...F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing...Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction...

  5. Pooled Library Amplifications

    Type
    Blog Post
    ...overexpression library) or is prone to recombination, we recommend amplification on solid media. If your plasmid ...recombination. If you do see recombination, we recommend ensuring the cells used are meant for repetitive...when developing your amplification protocol! We recommend looking at protocols used for libraries that are...

  6. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...Cell lysates are then treated with benzonase immediately before iodixanol purification. This approach ...production tips or tricks? Please share them in the comments below! Special thanks for Dr. Dan Stone and...AAV production and purification! References 1. Aurnhammer, C., Haase, M., Muether, N., Hausl, M.A., Rauschhuber...

  7. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...results to a standard curve. For CNV assays, it is recommended to use a reference sample with two copies of ...Taqman chemistry (see image, left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains... 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR. Basic principles of real-time...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them in ... effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

  9. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...beta-lactamase prior to division, instead of immediately experiencing the effects of the antibiotic. This...little more time, since bacteria won’t divide immediately after it has been heat-shocked or electrically...Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley, W. K., & Brooks, G. F. (1986). ...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...but they cannot be entirely eliminated. It is recommended that you image samples before and after expansion...representation of reality. Third, expansion results in dimmer samples. Reduced signal can be caused by loss of...https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical guide...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...purification kits, it is low cost and can be immediately adopted by any laboratory. All procedures are...new DNA fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone...This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than ...

  13. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...conjugated to agarose or magnetic beads with commercially available chemical agents. Directly conjugating...Protein A/G to immobilize the antibody, it is recommended to preclear the sample to remove any proteins...low pH buffer such as 0.1M glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine...

  14. The Challenges of Cell Culture

    Type
    Blog Post
    ...the difference between materials available from commercial repositories and the same cell line from a colleague...difference is the QC and validation carried out by the commercial repository. Repositories generally ensure cell...mutation rate of the cell line, but it is broadly recommended that the lower the passage number the better ...

  15. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... the two fluorophore colours. Experts in FRET recommend to using two fluorophores that have a similar ...one that is better than another. It is often recommended by FRET experts to use as many measurement methods... Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann T,  Terjung S. FRET Basics and Applications: ...

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...corresponding fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated...this system is that streptavidin conjugates are commercially available for a wide range of fluorophores, ...on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling of...

  17. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...IPI’s anti-integrin rAbs are unconjugated and recommended for use in flow cytometry. Based on the established...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...

  18. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...crossed strand. While this pathway may not be immediately applicable in DSB-induced genome engineering,... the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA templates can be used...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech....

  19. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...with almost 80% rating it as very important. One comment reflected the desire for a flexible generic solution...Important collaborative functionalities include comments, notifications, sharing files, assigning team ...actions. These records can take the form of written comments, added files, notes etc. Detailed records about...

  20. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...DNA fragments. As a fun way to start, I highly recommend watching this entertaining video created by our...at 50 °C. After an hour or so, the sample is immediately ready to transform into competent cells. The ...contain the appropriate homologous sequences. NEB recommends an overlap of 15-40 bp, with a primer melting...
Showing: 141 - 160 of 560 results