We narrowed to 565 results for: mme

...optimization. Before beginning an experiment, it is recommended that you try a few different ratios of transfer...will need to be optimized prior to starting. We recommend calculating the ratio of the surface area of the... target cell line. To overcome this issue, we recommend using a range of doses of lentivirus when transducing...

...you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG...machine fits on a table top and can be easily programmed for a variety of lab needs. Addgene uses it to... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...

...the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior to their...of cell culture medium on the packaging cells immediately following transfection. In other words, when ...

...trying to strip and reprobe. In this case, we recommend using mild stripping solution (see below) to minimize...Run your gel and membrane transfer as normal. Immediately after transfer, dry the membrane to maximize ...retention. Following this step, most PVDF membranes recommend re-wetting with methanol before rinsing with wash...

...results to a standard curve. For CNV assays, it is recommended to use a reference sample with two copies of ...Taqman chemistry (see image, left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains... 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR. Basic principles of real-time...

...Cell lysates are then treated with benzonase immediately before iodixanol purification. This approach ...production tips or tricks? Please share them in the comments below! Special thanks for Dr. Dan Stone and...AAV production and purification! References 1. Aurnhammer, C., Haase, M., Muether, N., Hausl, M.A., Rauschhuber...

...trained our models in close collaboration with the Krauthammer lab (University of Zurich), especially with my...F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing...Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction...

...overexpression library) or is prone to recombination, we recommend amplification on solid media. If your plasmid ...recombination. If you do see recombination, we recommend ensuring the cells used are meant for repetitive...when developing your amplification protocol! We recommend looking at protocols used for libraries that are...

...beta-lactamase prior to division, instead of immediately experiencing the effects of the antibiotic. This...little more time, since bacteria won’t divide immediately after it has been heat-shocked or electrically...Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley, W. K., & Brooks, G. F. (1986). ...

...but they cannot be entirely eliminated. It is recommended that you image samples before and after expansion...representation of reality. Third, expansion results in dimmer samples. Reduced signal can be caused by loss of...https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical guide...

...reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them in ... effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

...purification kits, it is low cost and can be immediately adopted by any laboratory. All procedures are...new DNA fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone...This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than ...

...company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time when...sometimes teams disagree. Yes, sometimes work is a bummer when you’d rather be at the beach or binging a ...

...conjugated to agarose or magnetic beads with commercially available chemical agents. Directly conjugating...Protein A/G to immobilize the antibody, it is recommended to preclear the sample to remove any proteins...low pH buffer such as 0.1M glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine...

...the difference between materials available from commercial repositories and the same cell line from a colleague...difference is the QC and validation carried out by the commercial repository. Repositories generally ensure cell...mutation rate of the cell line, but it is broadly recommended that the lower the passage number the better ...

... the two fluorophore colours. Experts in FRET recommend to using two fluorophores that have a similar ...one that is better than another. It is often recommended by FRET experts to use as many measurement methods... Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann T,  Terjung S. FRET Basics and Applications: ...

...IPI’s anti-integrin rAbs are unconjugated and recommended for use in flow cytometry. Based on the established...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...

...with almost 80% rating it as very important. One comment reflected the desire for a flexible generic solution...Important collaborative functionalities include comments, notifications, sharing files, assigning team ...actions. These records can take the form of written comments, added files, notes etc. Detailed records about...

...corresponding fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated...this system is that streptavidin conjugates are commercially available for a wide range of fluorophores, ...on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling of...

...crossed strand. While this pathway may not be immediately applicable in DSB-induced genome engineering,... the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA templates can be used...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech....