...subcellular localization and composition of cellular complexes are important steps in understanding the ...Once you've created your fusion protein, it's important to validate its function relative to the untagged...experiments in a scientific and meaningful way it is important to choose the appropriate quantification methods...require some consideration of FP properties. It is important to look out for general problems associated with... for fluorescently tagged viral and bacterial components. For example, the Rainbow Vectors from the Mariette...

...Preparing for the first meeting There are many important pieces before that first meeting takes place to... to ensure success. One of the most important is letting people know it’s happening. Aim for 3-4 weeks...technical dive on single papers could be more important.  Any slides, presentations, recordings, and other...yourself in this situation, considering stopping (temporarily or permanently) the recurring meeting or perhaps...be well planned and carefully organized. Most importantly, they should be regularly assessed for continued...some of the information described above you feel empowered to start your own journal club! Additional resources...

...antibiotic compounds. In such a case, the microbe itself would be unharmed by the compound it produces...this post will, indeed, focus on bacteria, it is important to note that the term ‘antibiotic’ can also be...treat infections, we are treating microbes with compounds similar to those they have been battling for centuries...animal farming is already being recognized as an important step, as you can’t walk into any supermarket now...hygiene standards. Handwashing is an essential component of preventing infection, as is cleaning and disinfecting...

...point is there are many ways to quantify the composition of a viral vector solution, and generally these... characteristics give us information on three important factors. What can AAV titers tell you? Physical...dosing purposes in preclinical studies, it is important to understand what these values mean and how they...low levels of background protein and chemical components, ddPCR may not be an improvement over qPCR (Taylor... lab. The absolute titer values may not be as important as the relative titer values. Finally, because...PMC2900506. Additional Resources on the Addgene Blog Important Considerations When Using AAVs Addgene's AAV Quality...

... are more likely to consider cover letters an important part of the application. Even within an organization...core skill, considers cover letters to be very important. On the other hand, our app/dev team rarely, if...that organization or group. This is particularly important for labs and nonprofits, who tend to prioritize... research], and have excellent [list the most important skills for the position]. I am enthusiastic about...about [something that you love and is important for the job]. Notice that the intros above allow you ...that they considered cover letters to be more important for higher-level positions. Many did not require...

... of working in industry and how delegating can empower people Chapter 3: How Crystal advanced at Novartis...Chapter 4: Crystal's approach to management and the importance of realizing that different people have different...projects, and how she sets metrics for success. The importance of thinking about the big picture and not just... who are planning on working in industry. The importance of recognizing and leveraging your unique skills...skills and how they've helped her at Novartis. The importance of mentorship in developing career skills. Outro...

...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it does...genetic disease in a postnatal mouse model, an important proof of concept for future preclinical and clinical...desirable. Should stem cell editing occur, the CRISPR components would only need to be expressed for a short period...does not cause obvious germline modification, an important finding given the controversy associated with ...great step forward for CRISPR gene therapy, it’s important to realize that DMD is a simpler case than other...

...orchestrate the formation of high-functioning-but-temporary teams that have a specific purpose and finite ...year, or a few months, or even a few weeks. The important thing to remember is that every team should have...when I do come across one. Also, remember, one important feature that all projects have in common - and... What does a good team need? Besides that all-important sense of purpose, there are many more things necessary... function is certainly necessary, but equally important is making sure all team members understand what...their responsibilities are. That’s a subtle but important distinction. If I invite someone to be on a project...

...because it will be difficult or (more likely) impossible to find; therefore all the time spent collecting...t guarantee a publication. There is an art to composing manuscripts and the ASM Scientific Writing and...writer, I was unsure if I should possibly take a composition class in the English Department. He suggested... coming to the writing workshop in D.C. had to compose and submit a manuscript that they were working .... Practice writing everyday Overall, the most important instruction the classes taught me was to write...

...understand the specific components that make up a plasmid and why each is important. Our “Plasmids 101” series...molecular biology knowledge and techniques, and empower you with a firm understanding of the fundamentals...Drives transcription of the target gene. Vital component for expression vectors: determines which cell ...and selectively grown on antibiotic plates.  Importantly, because the bacteria from which plasmids are...

...from one man teams.  The composition of the management team is more important than the product itself ...CEO of miLab, a Boston-based startup focused on empowering early-stage scientists. You can think of miLab... that raising capital for such a fund is an impossibility at our early stage.  After each pitch we made...diverse educational backgrounds (an essential component to any successful startup management team). Sreyan...

...warfare [Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling ...nonspecifically soak up your fluorophore package. One important concept in FRET experimental design is that the...donor first where labeling specificity is most important. Donor labeling and the need for specificity Donor...protein molecules contain the donor and, more importantly there is no fluorescence background resulting...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...

...biosensor with two components - an enzyme that breaks down the HMO of interest into its component simple sugars...particularly notes their ability to be precisely spatio-temporally controlled as a key advantage over other inducible...learn more about Xin’s work with photoswitchable components of the ERK signaling pathway in our recent blog...Oligosaccharides (HMOs). HMOs are thought to play an important role in nuturing the development of beneficial...

...responsible for the degradation of over 600 cellular components ultimately leading to cell fragmentation and ... fluorescence range to better study the spatio-temporal dynamics of apoptosis in living animals. To do...and (3) β-strand 11 (β11). Each part contains components and residues that are necessary for fluorescence...zebrafish, the Shu Lab used FlipGFP to track the spatiotemporal dynamics of apoptosis during embryo development...allow scientists to observe both the spatial and temporal dynamics of apoptosis both in vitro and in living...

...enough DNA and competent bacteria to ensure all components of the library are represented. Typically, Addgene...may be amplified preferentially, skewing the composition of the final recovered DNA. This bias can be ...need to count how many times each of the library components is found in the read list. If you also sequenced...give you a bell curve of the abundance of each component of your library. You should look for two things...hope this post has helped clarify some of the important considerations when using, amplifying, and verifying...

...secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium, a panel...might be able to attend the 10 Years of iPSCs Symposium earlier this year, I jumped at the chance. Here... most scientists recognize and appreciate the importance of ethics, the topic can often take the back ... the stem cell community for addressing these important questions that the scientific method cannot address...

...not E. coli. They fall under the heading of two-component systems (TCSs). TCSs consist of a histidine kinase...created the first E. coli light-sensitive two-component system (TCS). Levskaya et al. fused a photosensory...gene expression. An E. coli light-sensitive two-component system (TCS). A hybrid histidine kinase/photosensor...experimented with the promoter strength of various components, removing inducible promoters that could crosstalk...optimization of light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ....

...believe that communication is the single most important skill that scientists need to succeed in their... always recognized and valued for its immense importance, it may well be what determines whether you get...wasted if you cannot convince others that it is important and relevant to them.   When you think about it...you will also have to explain why the work is important and how it should change how the members of your...fields become excellent science communicators. To empower graduate students to become ambassadors for their...

...another way to activate gene expression, and two-component systems used in synthetic biology use light to...Addgene also has yellow flame light-regulated two component systems designed by the Tabor lab. Which inducible...promoter is right for me? Here’s a list of some important variables to consider when choosing an inducible...’re expressing a gene that may be toxic, it’s important that your inducible promoter not be too leaky....Region Plasmids 101: Cre-lox Light-Switchable Two-Component Systems for E. coli Browse other blog posts from...

...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...the linearized vector by T4 digestion; then the components are combined and annealed. The resulting recombination...BioRender.com.   SLIC also opened up a new world of multicomponent assembly, as fragments with overlapping sequence...efficiency (~20%). Figure 2: Schematic of multicomponent assembly with SLIC. Multiple fragments are ...issue is sequence similarity. If fragments in a multicomponent assembly have 5′ or 3′ sequence homology to...