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TypeBlog Post...modified monomeric red fluorescent protein (RFP), named GEMCherry2, that can rapidly and efficiently evaluate...methionine start site. This first iteration was named GEmCherry1, due to the “genomic fragment inserted...
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Viral Vectors 101: Biosensors
TypeBlog Post...biology you are studying. For example, calcium dynamics in the cytosol and sarcoplasmic reticulum are ... to stimuli and as well as changes in cellular dynamics during so-called housekeeping activities. ... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post... matches of a name in the depositor field will rank higher than matches of the same name in the publication... -
Plasmid Tools for Microbiome Studies
TypeBlog Post...gut microbiota Monitoring microbial population dynamics in the gut can provide insight into how the microbiome...distributed cell division counter reveals growth dynamics in the gut microbiota." Nature communications ... -
Antibodies 101: Affinity Reagents
TypeBlog Post...needed steps to continue where the guide left off - namely, a blog post devoted to the other affinity reagents...Single-domain antibodies (sdAbs), sold under the commercial name nanobodies, are derived from a camelid antibody ... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...Note that many antibodies are sold under different names through multiple vendors, so look at epitope target... between the same antibody sold under different names. If all else is equal, feel free to make the decision... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...Find as many opportunities as you can to get your name on things.” Susan personally found this easier to...“If you use it right, it could help you get your name out for stuff that you’ve written.” Ultimately, ... -
CRISPRainbow and Genome Visualization
TypeBlog Post...Stanley Qi and used in Chen et al. to track telomere dynamics throughout the cell cycle. To optimize the signal...Addgene. References 1. Chen, Baohui, et al. “Dynamic Imaging of Genomic Loci in Living Human Cells by... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...all of my papers and they all have filenames (year first author name). It’s rudimentary, but it works for... -
Plasmids 101: Cre-lox
TypeBlog Post...tags, and antibiotic resistance markers (just to name a few). In this edition, we’re going to take a look...genome manipulation. Cre recombinase, originally named because it “causes recombination” (although later... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...articles published there as well. Worm Development Dynamics Database (WDDD) The WDDD aims to acquire quantitative... Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41.D1 (2012)... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...CRY2clust enables faster assembly and disassembly dynamics, higher sensitivity to light, and better distribution...super-resolution techniques for live-cell imaging of the dynamic changes in biochemical activity within the cell... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog Post...social biomolecules. They can be part of multiple dynamic and overlapping complexes that have distinct functions...particularly useful for in-depth characterization of dynamic protein complexes. Split-BioID, like BioID, is ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...anti-CRISPR nomenclature Acr family proteins are named for the type of CRISPR-Cas system they block and...light, or small molecules could achieve rapid, dynamic, and spatial control of CRISPR-Cas activity. For... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...components: a deaminase (TadA, originally from E.coli, named TadA7.10 in ABE7.10) a catalytically impaired Cas... ABE7.10 accordingly which resulted in variants named “ABE8.x”: “8” stands for the eighth round of ABE... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...ligand co-developed by Bryan Roth's and Takafumi Minamimoto's lab called deschloroclozapine (DCZ) shows promise...Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide, clozapine, and... -
Tips to Make the Most of a Scientific Conference
TypeBlog Post...want to include your university and your advisor’s name for some context and maybe common ground. The people...also helpful to write your Twitter handle on your nametag for the tweep up (and for the entire conference... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...differentiation. CAPTURE 2.0 captures chromatin's dynamic role in gene regulation The high resolution and... capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated... -
Antibodies 101: Flow Compensation
TypeBlog Post...compensate between them. Another rule of thumb is if the names of the fluorescent markers are similar, you will...Chart. Pro tip! Some antibodies go by different names depending on your flow cytometer’s setup. For instance... -
Polymerase Chain Reaction Overview and Applications
TypeBlog Post... analyzed. End point PCR End point PCR, as the name implies, analyzes the end product of PCR temperature...Quantification.” Multiplex PCR Multiplex PCR, as the name implies, is a method in which multiple targets can...