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We narrowed to 388 results for: nans

Showing: 141 - 160 of 388 results

  1. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ..., Bjorck 1984). Protein A, Protein G, or the recombinant Protein A/G are conjugated to agarose or magnetic...their primary antibody. To circumvent this, a recombinant protein, Protein A/G, was developed, which can...on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene  ...

  2. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...has created an automated tank for feeding and maintenance that had the audience gasping. Animal husbandry...the number of plants you need to grow to get a recombinant because this process is expensive. They are using...fluorescent indicator system to rapidly identify recombinants. Arthropods (Jason Rasgon) This lab is developing...

  3. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...non-specific binding or false positives.  If testing a recombinant protein, especially one with tags, be sure to...the endogenous protein, since folding of the recombinant protein may differ from the native form, which...addgene.org The Addgene Antibody GuideAddgene's recombinant antibody collection...

  4. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...results in the production of air-filled protein nanostructures called gas vesicles, which scatter sound waves... acoustic contrast in E. coli. We deleted an unannotated hypothetical protein, improving expression, and...Maresca, D. et al. Nonlinear ultrasound imaging of nanoscale acoustic biomolecules. Appl. Phys. Lett. 110, ...

  5. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...to the daily news briefing, to quick notes, to finances, to flights & ordering food - everything is covered...significant barrier to ELN adoption. This includes financial outlay, staff hours, troubleshooting, and the ... long-term use is likely to require on-going maintenance and support.” Besides that, academic institutions...

  6. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...When to Use Them Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide... software Reagents Purified antigen Purified recombinant antibody Isotype control antibody HRP-conjugated...plate was coated with serial dilutions of human recombinant purified Desmin (blue) or a negative control ...

  7. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Addgene's own empty lentiviral backbones New recombinant antibodies for Glypicans DNA editing with STITCHR...11012), and counterstained with DAPI. New recombinant antibodies targeting Glypicans By Ashley Waldron...) recently released a set of highly-specific recombinant antibodies against three of the six human glypican...

  8. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ... Central PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction and expression of genes...expression and largely defines the amount of recombinant protein that will be produced. Many commonly-...

  9. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...components can be electroporated into the oviduct of pregnant females, a technique called Genome editing via...injection into either an oocyte or into the oviduct of pregnant females (Yoon et al., 2018, Mizuno et al., 2018...in vivo genome editing in mouse embryos using recombinant adeno-associated viruses. Nat Commun 9: . https...

  10. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...and should not be confined by birth country or financial means. Instead, talent should be realized through...globalized environment re-emerging from international financial struggle, our new generations feel thrown, to ...twitter @zcastan or on linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard...

  11. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...largest source among those mentioned above. The contaminants can spread through dirty clothing, lab wears...should be made available for cell culturing and maintenance of the cell lines. Providing such an environment...for the lab to avoid bringing in environmental contaminants.  Figure 2: An Addgenie works in the...

  13. Important Considerations When Using AAVs

    Type
    Blog Post
    ...and Richard J. Samulski. "Self-complementary recombinant adeno-associated virus (scAAV) vectors promote...-limiting step for efficient transduction by recombinant adeno-associated virus vectors." Journal of virology...10. Wu, Jianqing, et al. "Self-complementary recombinant adeno-associated viral vectors: packaging capacity...

  14. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...imaged with stimulated emission depletion (STED) nanoscopy. Scale bar, 2 μm. Inset shows comparison with ...& Bewersdorf, J. (2016). Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications... R. Y. (1998). Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells. Science,...

  15. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...light (~400 nm), the chromophore photoconverts predominantly to the anionic form, causing increased fluorescence...multilabel imaging and dual color fluorescence nanoscopy." Nature biotechnology 26.9 (2008): 1035-1040....fluorescent proteins for far-field fluorescence nanoscopy." Biophysical journal 95.6 (2008): 2989-2997. ...

  16. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...EcoFlex a great option for those interested in recombinant protein purification and metabolic engineering...expression, the system includes a toolkit to express recombinant proteins within the mitochondrial matrix.   ...localization and transport studies in HEK cells, recombinant protein purification in bacterial cells, and ...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator allele of the E. coli MMR protein...and SnoopTag/SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists...wine, fuels, pharmaceutical ingredients, and recombinant proteins. In order to keep production yields ...containing long homology arms and both auxotrophic and dominant selection markers, allowing for use with both ...

  18. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...collection of IPI’s well-validated and ready-to-use recombinant antibodies. The first collection, a set of integrin...sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access to IPI...

  19. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...manufacturer. You can also consider using monoclonal or recombinant antibodies, which have much lower lot-to-lot ...point. You can also consider using polyclonal or recombinant multiclonal antibodies to increase overall signal...enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not properly...

  20. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... their reagents: detection methods, synthetic nanobodies, SARS-CoV-2 protein expression, and more. We ...Quantification Methods to Consider by Jennifer Tsang The nanodrop vs. spec debate dominates DNA quantification discussions...
Showing: 141 - 160 of 388 results