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Showing: 141 - 160 of 783 results

  1. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    ...scientists up to on Twitter? Prior to writing this post, my interest in Twitter was fleeting. I’ve had an...8 tweetable facts. Note: The images used in this post were created using data from or modified from Ke...Biologists, neuroscientists, and ecologists (orange box) post tweets containing scientific domains more often ... use social media as a scientist? Check out this post. Resources on the Addgene Blog 6 Tools & Tips:...

  2. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...antibiotic resistance marker). In a previous blog post, we covered how to use positive and negative selection...are blue. Check out our blue-white screening blog post for more information.   Figure 1: Result of... process, or visit our restriction analysis blog post. Colony PCR Colony PCR can detect the presence...details and learn more about colony PCR in this blog post.   Figure 2: The steps of colony PCR.   ...either terminus. Want to know more? Visit this blog post for tips for analyzing and troubleshooting Sanger...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...

  3. The time and cost required to make a plasmid

    Type
    Blog Post
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...million plasmids shared milestone in 2018, we wrote a post on the time and cost required to make a plasmid.... not!), we think it’s about time we updated this post. How much time does it take to make a plasmid?...precise calculations for any of the numbers in this post is a mathematical story well beyond my ability to...help you choose the right cloning method Find blog posts about scientific sharing here Download the Plasmids...save yourself hours by chatting quickly with the postdoc who’s really good at primer design – but first,...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...elements for vector propagation. This post, along with a future companion post on mammalian vectors, will catch...In our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance...of plants and animals. Unlike bacteria, yeast can post-translationally modify proteins yet they still share...Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed how the regulation of bacterial...

  5. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...biology blog posts Find tips for Arabidopsis transformation Read our Plasmids 101 blog posts Resources ...This post was contributed by Samuel Mortensen, a PhD candidate at Northeastern University. Working with...transient technique that uses a virus and the plant's post-transcriptional machinery to silence genes of interest...MoClo system. The vectors mentioned in this blog post above are ready to use vectors, but with the MoClo...and Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene silencing method." International...

  6. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...connecting. You can see what they post and occasionally comment on their posts as appropriate, possibly giving...companies and organizations to see their news and job postings. 4) Use LinkedIn to pay it forward The best way...profiles. 7) Keep it professional at all times Do not post or comment on anything that is not relevant to business...for using social media as a scientists Read blog posts about networking ...will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile” instead of “...

  7. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...CRISPR blog posts Read blog posts about using Cre-lox Read our genome engineering blog posts Resources...seem endless - and overwhelming. In the first blog post in this series, I’ll highlight terminology you should...mouse seems less overwhelming! In the second blog post of this series, I’ll discuss crossing and maintaining...

  8. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    ...lower the probability of off-target editing. In this post, we’ll summarize how IDT (Integrated DNA Technologies...nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the RuvC domain, ...donors (if possible) The text and images in this post were adapted from an IDT webinar: Optimized methods...Mollie Schubert and Shuqi Yan for reviewing this blog post. Additional Resources on the Addgene Blog Learn...

  9. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...tools not listed in today’s post? Share them in the comments below. This post was written in April 2020 ...virtual molecular biology toolbox organized, today’s post features a list of free online molecular biology...functional information, such as expression patterns, post-translational modifications and polymorphisms Codon...

  10. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...In this post of the Careers in Science Communication blog series, you’ll hear more about Caitlin Runne-Janczy...how Caitlin got into scicomm and landed her first post-grad school job, head over to part I of the interview... proposals, a few PhD theses, and occasionally posting something science-related on Facebook. “I think...Channel of the blog so you don’t miss the future posts in this series! Additional Resources on the Addgene...

  11. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...about what makes a good mentor, see the previous post in this series. Check out Joanne's Reddit AMA Different...provided a downloadable PDF at the end of this blog post. This document includes a list of questions to ask...Academic Mentors by @drmellivora, an excellent blog post about workplace bullying featured on the always ...developing your career in science.  Read other posts in our Science Mentors series: Part 1: "What Makes...shocking how little pre-work most PhD students and postdocs do to ensure the advisors they choose will be ...Different types of mentors for grad school vs. postdoc Choosing an advisor to complete a successful PhD...PhD is different from choosing a lab for postdoctoral work. However, selecting a lab with a PI who is ...

  12. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...creative uses of old posters, pipette tip boxes, and more (stay tuned for a few blog posts coming up about ...Eppi the red nosed reindeer.       View this post on Instagram     Eppi the red nosed Reindeer! ...research #cardiovascularresearch #parkinsonsdisease A post shared by Ladies in labcoats (@ladiesinlabcoats...

  13. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...scicomm ♬ original sound - Addgene We'll be posting these animations regularly from now until May, ...follow us to get updates whenever a new video is posted! If TikTok isn't your platform, all of our videos...

  14. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...active under specific circumstances. In a previous post, we discussed inducible promoters, which can be ...either category, but those are a subject for a future post! How are repressible promoters regulated? Like...as the inducible manner detailed in our previous post. In the past 30 years since the original Tet-Off...as the inducible manner detailed in our previous post. While originally developed for use in mammalian... modular vector for ease of use! Conclusion This post contains only a brief overview of some of the most...The repressible promoter systems detailed in this post can also be combined with other systems, such as... what might work best for your experiments! This post was originally written by Mary Gearing in 2018, ...

  16. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... Research Toolbox blog posts on free tools for making scientific graphics post and social media for scientists...going on in other areas of science. Scientists often post tweetorials, or threaded summaries, of their papers...topics.  Twitter paper bots: Twitter bots tweet posts that contain specific keywords. It’s sort of like...covered four ways to find relevant papers in the post, there are many more ways out there. Let us know...

  17. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    ...Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides...infrastructure, and secure collaboration.  In that post, we talked about Addgene’s data processing needs...

  18. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...Other Career Blog Posts Read the Networking for Scientists Series Read our Blog Post about Choosing a ...This post was contributed by guest bloggers and IMP organizers Rosario F. Godino, Rocío López-Diego, ...expectations of the program and their baseline skills and a post-program self-assessment, participants completed ... results. Participants’ responses to the mid and post-program self-assessment revealed that their knowledge... degeneration. She is also a blogger who writes posts for the blogs of MasScience, ECUSA, IMFAHE and Bostonadas...linkedln.       Rosario Fernandez-Godino is a Postdoctoral Researcher at MEEI-Harvard Medical School, where...

  19. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...research. When you're done with this post, check out our follow up post on repressible promoters. How are...only active under specific circumstances. In this post, we’ll discuss inducible promoters, which can be...Two-Component Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org...

  20. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...This post was contributed by Nikolai Braun and Keira Havens, co-founders of Revolution Bioengineering...Bioengineering. Read their previous blog post about how they started their company here. The first transgenic plant...Biophysics from UC Davis in 2007. Nikolai trained as a post-doc at the Biochemistry Department at the University...Like RevBio on Facebook Read more Addgene blog posts about synthetic biology ...
Showing: 141 - 160 of 783 results