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We narrowed to 605 results for: sam

Showing: 141 - 160 of 605 results

  1. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...produce the same signal, so only one HRP-conjugated RANbody can be used at a time. Samples can be sequentially... lower background and better penetration of the sample due to the smaller size of RANbodies compared to...sequentially stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible...it is approximately as sensitive as detecting the same antigen with a standard primary/labeled secondary...

  2. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...reason you can’t then ask that same question in your next interview. The same goes for answers interviewers...interview questions. Story telling shares many of the same elements as a STAR answer: a setting, a conflict...pay attention. Your interviewers will not have the same experiences as you, so set them up for success by...and your interviewers. You’re probably all in the same boat, but there are a few things you can do to make...

  3. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...affinity binding, such as immunoprecipitations. Your sample species should also be considered when choosing...immunoglobulins naturally present in the tissue sample. There are species-on-species staining protocols...secondary antibodies that are all derived from the same host species and have been cross-adsorbed. The cross-adsorption...using mouse IgG2a and rat IgG1 antibodies in the same application, using a Goat anti-mouse IgG2a cross-adsorbed... species, and secondary antibodies raised in the same species that have been cross-adsorbed to reduce ...

  4. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...high and above what would be expected in patient samples (e.g.: blood, urine). Adapting toehold switches...pathogen’s genome as unique barcodes to identify samples containing the virus. Of the 48 sensors, targeting... diagnostic results.  Our approach now detects samples at clinically relevant concentrations (1-3 fM; ... look at viral load in patient blood and urine samples; we showed that we can detect the virus directly...Interview with Previous Collin's Lab Member and Sample 6 Cofounder Michael Koeris Learn How Minigenomes...

  5. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...removing excess dye from the sample, which is particularly important for samples where washing is difficult...reason is something called “the photon budget”: each sample has a limited number of fluorophores and each fluorophore...of information one can extract from a biological sample is wholly dependent on the photon budget and pushing... desperate to extract a few more photons from a sample. Chemical fluorophores can be substantially brighter...

  6. Viral Production at Addgene

    Type
    Blog Post
    ...of the viral capsid proteins VP1, VP2, and VP3. Samples of viral preparations are subjected to polyacrylamide...proteins as a fraction of total protein present in the sample is also determined and used to determine purity...shown relative to the total protein present in the sample.   Sterility Viral vector preparations are ...rigorous barcode matching system that follows the sample from the DNA tube all the way to the cleared viral...throughout production, ensuring the identity of each sample from the DNA tube all the way to the viral preparation...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...future.  Many thanks to our guest blogger Samantha Young. Samantha Young is a Medical Writer with a PhD in... This post was contributed by guest blogger Samantha Young. The use of CRISPR/Cas9 for gene editing has...classified into four groups (4; brighter than control, 3; same as control (Cetn1/sgRNA1), 2; darker than control...technique, you can assess several sgRNA’s at the same time, and choose the most effective ones to increase...

  8. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...FACS achieves greater purity of your sorted cell sample than MACS. An effective strategy to sort out your... T cells (Figure 2). Even better, we can use the same fluorophore for the three lineage markers we have...gating labels all unwanted cell lineages with the same fluorophore to easily gate on the unlabeled cells...different while the permeabilization solution can be the same). Examples of such targets include transcription...undesired cell populations. Dump gating uses the same color for different markers, making it easy to gate...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ... different phenotypes of the same cell type, are intermingled in the same brain nucleus: a serotype that...tropism between animal species or even strains of the same species. What works well in a mouse may not work...doi.org/10.3390/cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-...10.3390/pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-...

  10. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...phenomenon wasn’t until much later when, in the 1960s, Osamu Shimomura studied the bioluminescent properties ... of the coast of Washington state. Using these samples, he was able to isolate two proteins from the jellyfish... fluorescent proteins with new colors Around the same time, researcher Sergey Lukyanov realized that, ...organisms to find the culprits. He even obtained samples of certain ones from local pet shop aquariums in...

  11. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...should receive the clinical sample to test their phages. Because the sample is limited, only the labs that...bacteria and sometimes, only a subset of strains of the same bacterial species are affected. As of now, phage...targeting organisms closely related to the clinical sample. For treatment, it is best to identify a cocktail...

  12. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ... last step will influence the stability of your sample over time and determine which experiments you can... impact the time you will be able to store your sample without having substantial degradation and which... DNA, it will limit what you can do with these samples. This is because divalent cations are essential...reaction, an effective possibility is to dilute your sample in water to reduce the EDTA concentration. Alternatively...

  13. Fostering communication in remote collaborations

    Type
    Blog Post
    ...asynchronously. The best way to keep everyone on the same page is to make communication a non-negotiable part...meeting, but short enough that everyone stays on the same page. Ideally, these meetings include all collaborators...documents While having multiple scientists edit the same document may sound like something out of your nightmares...or ensure that techniques are being performed the same way at different sites. I did a lab exchange as ...

  14. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

    Type
    Blog Post
    ...features and it allows you to more quickly identify the same feature in other plasmids. Aligning sequences with...allows us to quickly align multiple sequences at the same time. This means we can easily compare and analyze...Snapgene-powered plasmid maps and sequences use the same feature detection software as the desktop version...analyze the sequence data in depth. You will see the same features annotated in our plasmid maps, including...

  15. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ... plasmids with the same ori are incompatible because they will compete for the same machinery, creating...unpredictable environment. As a rule, plasmids from the same group should not be co-transformed, so if you need... an arbitrary designation, and plasmids from the same incompatibility group should not be co-transformed...

  16. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...good communication is making sure everyone’s on the same page. So for our first post, I thought it best to...communication by and to scientists in (roughly) the same field, often through manuscripts, seminars, or posters...PCR test” to mean “a test that uses a biological sample to look for presence of a virus.” A scientist would...consistent and clear.   And now that we’re all on the same page, we’re ready to get into the more exciting ...

  17. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ... aim to do large scale DNA preps with multiple samples without cross contamination. We currently use the...create a DNA library, one must first fragment a DNA sample into many small components. Without proper processing...process with, we got great amplification of our sample libraries. NEB also gave us a few goodies which...my first blog post! I highly recommend doing the same if you ever go to a workshop because it will help...

  18. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...this information, however, did not improve at the same pace as the quantity and quality of the information...mechanism to “collapse” variations and synonyms of the same term that appear in our data into a single filter... Matching We capture multiple variations of the same information in our search indices in order to support...depositor field will rank higher than matches of the same name in the publication field. Suggestions We provide...

  19. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... A: It is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore...somewhat more reliable quantitation. Differences in sample treatment across methods can affect reported titer...that users keep titering method consistent across samples within a project. Targeting Your AAV Q4: Are there...of co-delivering different AAV particles into the same cells in vivo? A: This will be highly dependent ...

  20. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...Monoclonal” refers to antibodies that are all of the same isotype and specificity. In other words, monoclonal...recognize the protein even though it is present in your sample. Additionally, there is a lower likelihood that...antibodies that recognize many different epitopes on the same protein - are more likely to have off-target effects...produces high-affinity/high-avidity antibodies. The same hybridoma is then used to produce all lots of the...
Showing: 141 - 160 of 605 results