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  3. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ... the protein as it exists in your assay?  First, try to understand, to the best of your ability, what ...protein that is being used as a marker for flow cytometry, for example, can only be recognized by antibodies...samples. If, however, you are performing an immunohistochemistry assay, you may want to rule out monoclonal...

  6. Hot Plasmids - October 2022

    Type
    Blog Post
    ...actively testing it in other applications! If you try it out in a new assay, let us know. We’d love to ...

  12. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...bd-spectrum-viewer.   To make life easier, always try to use fluorophores with distant excitation/emission...bleeding, or spectral overlap, and why you should try to avoid or reduce it whenever possible. Finally,...Introduction to Gating in Flow Cytometry Antibodies 101: Flow Cytometry Antibodies 101: Flow Compensation...When analyzing your cells using flow cytometry, you are typically measuring the presence or absence of...reporters, and/or dye stains used in your flow cytometry experiment are known as your "panel". In this ...from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover Excitation...degree. The phenomenon of spectral overlap in flow cytometry is commonly referred to as bleeding. When two ...

  13. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...have mastered this technique so it might be worth a try, especially since the potential rewards could be ...continually being developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies...reassignment–Expanding the chemistry in biology." Chemical Science 6.1 (2015): 50-69. Royal Society of Chemistry. 3. Ermolenko...distance between the donor/acceptor fluorophores (Stryer, 1978), a measurement that is unaffected by intervening...23667515. PubMed Central PMCID: PMC3647052. 11. Stryer, Lubert. "Fluorescence energy transfer as a spectroscopic...spectroscopic ruler."Annual review of biochemistry 47.1 (1978): 819-846. PubMed PMID: 354506. 12. Vilensky...

  18. Plasmid Cloning by PCR

    Type
    Blog Post
    ...your primer length and increase the Tm if you are trying to clone from genomic DNA, a cDNA library, or by...
Showing: 141 - 160 of 537 results