We narrowed to 333 results for: uros

1. 10 Great Guest Posts We're Thankful For

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   ...and chemogenetics communities to activate neurons and neuronal pathways using light and chemicals respectively...

2. Donations from Addgene to Yield Answers for Rare Disease Researchers

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   ...sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they now think that...that mutations in the gene may prevent neurons from returning to basal conditions normally after periods...

3. The Importance of High Titer for AAV Transductions

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   ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

4. Celebrate National Book Month with Addgene

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   ...Tales of Music and the Brain by Oliver Sacks "Neurologist Oliver Sacks examines the relationship between...Musicophilia includes case studies of relevant neurological atypicalities, including a patient with amusia...

5. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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   ...serotonin is a well-known but poorly studied neuromodulator, and evidence suggests that it is incredibly...the question of whether firing of serotonergic neurons (measured using a red calcium sensor) leads to ...

6. FlipGFP, a novel fluorescence protease reporter to study apoptosis

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   ... treated HeLa cells expressing FlipGFP with staurosporine, which induces apoptosis by activating caspase...array of diseases and pathologies such as neurodegenerative diseases, autoimmune disorders, and cancer...

7. Improving Transduction Efficiency with Exosome AAVs

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   .... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...stromal cells after intravenous delivery." Journal of neuro-oncology 139.2 (2018): 293-305. PubMed PMID: 29767307...

8. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

9. Building and Validating Recombinant Antibodies: A Case Study

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   ... recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed...of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative uses high-throughput DNA...

10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    ...Methods 2018. PubMed PMID: 30377362. Studying neuronal activity using the fluorescent voltage indicator...Listen to the voltage indicator podcast segment The neurons in the brain compute using electrical impulses ...

11. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...international, collaborative project—funded by the European Commission, the US National Science Foundation...increasingly, corporations in the US and Western Europe have taken three strategic approaches to gender...

12. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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    ...University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA. The primary focus of his lab’s ...University of Colorado Anschutz Medical Campus in Aurora, Colorado, USA.     Richard Gronostajski is a professor...

13. Hot Plasmids and Viral Preps - January 2021

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    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...

14. Hot Plasmids and Viral Preps - March 2021

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    ...cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   ...new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use...

15. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ... form of blindness known as Leber’s congenital amurosis (LCA). This disease is caused by mutations in ...central and peripheral nervous systems." Nature Neuroscience 20.8 (2017): 1172-1179. PubMed PMID: 28671695...

16. Plasmids 101: FLEx Vectors

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    ... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316...

17. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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    ...conserved in mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing ...

18. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...

19. Cloning Mammalian Cells with the Agarose Method

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    ...and endocrine cells such as AtT-20, PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in...of Medicine. Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical...

20. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation)...preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric...