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Showing: 141 - 160 of 337 results

  2. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...for successful transduction. In another example, neuronal gene expression by an anterograde AAV was shown...input-defined neural pathways for defense behaviors." Neuron 93.1 (2017): 33-47. PubMed PMID: 27989459. PubMed...

  5. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    .... Exo-AAV9 was successfully shown to transduce neurons in the brain after systemic (i.v.) delivery in ...stromal cells after intravenous delivery." Journal of neuro-oncology 139.2 (2018): 293-305. PubMed PMID: 29767307...

  6. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Tales of Music and the Brain by Oliver Sacks "Neurologist Oliver Sacks examines the relationship between...Musicophilia includes case studies of relevant neurological atypicalities, including a patient with amusia...

  7. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ... recombinant antibody resource called NABOR (Neuroscience AntiBody Open Resource). The antibodies distributed...of monoclonal antibodies publicly available. The Neuro Mab Sequencing Initiative uses high-throughput DNA...

  10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...international, collaborative project—funded by the European Commission, the US National Science Foundation...increasingly, corporations in the US and Western Europe have taken three strategic approaches to gender...

  12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ... form of blindness known as Leber’s congenital amurosis (LCA). This disease is caused by mutations in ...central and peripheral nervous systems." Nature Neuroscience 20.8 (2017): 1172-1179. PubMed PMID: 28671695...

  13. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...cell types including endothelial cell-pericyte, neuron-astrocyte, and cancer-stromal cell pairs.   ...new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now available as a ready-to-use...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral ...channelrhodopsin (C1V1) targeting parvalbumin cortical interneurons is now available as a ready to use viral prep...

  15. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ... morphology, and circuit mapping of a targeted neuronal population.    Figure 2 (modified from...activated and inactivated transgene expression in neurons.  PubMed PMID: 2286602. PubMed Central PMCID: PMC3406316...

  17. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...mitochondrial fission/fusion, nuclear import, and neuronal trafficking. Although FPs have enabled discovery...YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the Allen Institute ...

  18. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...and endocrine cells such as AtT-20, PC12, Rin5F, Neuro2A and GH4C1, among others (see Lindberg papers in...of Medicine. Iris Lindberg is a professor of Neurobiology and Anatomy at the University of Maryland Medical...

  19. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ...placing FP timer DsRed-E5 under the control of Neurog3 (a gene controlling pancreatic differentiation)...preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric...

  20. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are commonly ...GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab.   Figure 3: A knockout...
Showing: 141 - 160 of 337 results