Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 323 results for: virus

Showing: 141 - 160 of 323 results

  1. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ... enable the virus to enter host cells. To do this, they injected the spike protein from two different ...spike proteins in cell culture, and inhibit these viruses.  But llama immunization has its drawbacks: it...bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable...different coronaviruses into a llama over several days and isolated special camelid antibodies that bind to the...Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell ... carriers collection Find other COVID-19 and Coronavirus Plasmids & Resources ...

  2. CRISPR Cheat Sheet

    Type
    Blog Post
    ... that allow the bacteria to target and destroy viruses that infect them. Commonly referred to as a bacterial...

  3. Video Library

    Type
    Protocol
    ...Protocol AAV Purification Purify adeno-associated virus from a preparation produced in mammalian cells AAV...measure the physical titer of your adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green...

  4. Lentiviral Prep Service

    Type
    Collection
    ... controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can be used to interrogate the genome in...fluorescence. Morris Control Lentivirus Browse our list of available viruses based on plasmids in our repository...guarantee viral service, but lets us know what viruses would be helpful to the scientific community.... Viral Vector Packaging Service Lentivirus Browse In-Stock Lentivirus Pooled CRISPR Libraries Cas9 Pooled...Ready-to-use lentivirus available from Addgene's viral vector packaging service. Browse constructs currently...Pooled Barcoding Libraries Control Addgene's lentiviruses are made from plasmids that are currently in ... viral preparation from the inventory below. Lentiviruses are distributed as purified viral particles ...

  5. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...labs engineered the tobacco rattle virus (TRV), a bipartite RNA virus, to deliver a TnpB (named ISYmu1)...

  6. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...almost three decades, recombinant Adeno-associated virus (AAV) has been widely used to deliver genetic tools..., myelocytomatosis viral oncogene (myc), simian virus 5-derived epitope (V5), the synthetic peptide FLAG...

  7. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...-Associated Virus (AAV) While other viral vectors such as adenovirus (AdV) and lentivirus (LV) are known...Resources on the Addgene Blog Adeno-Associated Virus (AAV) for cell and gene therapy AAVs for genome ...prior with the death of Jesse Gelsinger in an adenovirus-based gene therapy clinical trial gone horribly...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    ...improve the catalytic efficiency of tobacco etch virus protease (TEV), a highly sequence-specific protease... from SARS-CoV-2 sequences and live influenza A virus. It was demonstrated to work in human lung epithelial...

  9. The Challenges of Cell Culture

    Type
    Blog Post
    ...propagate viruses in cell culture; if there were no cells to infect, there would be no viable viruses to study...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling methods that can be ...animals such as rats (17).  In addition, the use of viruses allows much better spatiotemporal control over ...

  11. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression promoter from the simian vacuolating virus 40 Constitutive  May include an enhancer. PGK1...Strong plant promoter from the Cauliflower Mosaic Virus Constitutive  Active in dicots, less active in monocots...mammalian expression promoter from the human cytomegalovirus Constitutive  May contain an enhancer region...expression mRNA  Strong insect promoter from baculovirus Constitutive  Commonly used in expression systems...

  12. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  13. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ...Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells....also like... AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction...allow full precipitation. Precipitation of the viruses can proceed overnight at 4 °C if needed. Transfer...cleared lysate to the tube containing the resuspended virus from step 12 above. Add 50 units of benzonase per...

  18. New Viral Vectors - March 2024

    Type
    Blog Post
    ...on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  19. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...Sontheimer EJ (2018) All-in-one adeno-associated virus delivery and genome editing by Neisseria meningitidis...and skeletal muscle of mice via adeno-associated viruses. Nat Biomed Eng 4:97–110 . https://doi.org/10.1038...

  20. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ... use in screening experiments; large amounts of virus are required to ensure that you get full library...backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR v2 is key ...
Showing: 141 - 160 of 323 results