...library of E. coli tRNA adenosine deaminases (ecTadA), fused it with dCas9, and transformed it into E. coli... (Komor et al., 2016 and Nishida et al., 2016) invented CRISPR-base editors by tethering cytosine deaminase...

...University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal deletions in human cells...efficiency of the method. One observation was an inverse relationship between deletion size and frequency...outcome to deletions following paired DSBs is inversions [3]. These rearranged segments may also be informative...nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

...10.1534/genetics.111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with...CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, ...testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This... the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting the...Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist named...which goes horribly wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting...

...recombination persists even in recombinase deficient E. coli and under conditions used to minimize recombination...Bourges D, Ersing I, Haery L, Harten DeMaio K, Sanders E, Tasissa M, Kostman M, Tillgren M, Makana Hanley L...reading frame is inverted in relation to the promoter, hence the name, double-floxed inverted open-reading...transgene is no longer able to flip back into the inverse orientation (See Plasmids 101: FLEx Vectors). While...opposite orientation (direction of arrows) the inverted transgene is reverted into the sense orientation...

...greatly simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary...gate cloning that results in the creation of many E. coli constructs with greatly varied levels of expression...well as a dominant-negative mutator allele of the E. coli MMR protein MutL, all under the control of the... MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range of strains...domains, but these domains often do not express well in E. coli without their partner regulatory domains. The...library and used this library to screen for genes involved in West Nile Virus (WNV) induced cell death. The...for their gene of interest. Although the parts involved in altering gene expression are relatively well...

... Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk...doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, ...results in effective gene delivery in mice. J Clin Invest 118:1955–1964 . https://doi.org/10.1172/jci34316...

....org/10.1126/science.1231143 Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender...doi.org/10.1038/s41598-018-33408-9 Mizuno N, Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi...easy. In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome...

..., A. I., Saper, C. B., Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman... plasmids — as DNA or bacterial stabs — ‌and reinventing our search experience so you can find exactly...

...synthesizes the critical amino acid). Many common E. coli strains used for cloning are auxotrophic for.... Fortunately, there are many ways to do this involving positive selection, negative selection, and/or...

...grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping Beauty transposons rapidly...) The transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest ... enzyme is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction ...