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Showing: 161 - 180 of 422 results

  1. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    ...green cloud) functions both as a restriction endonuclease and as a ligase by cleaving and rejoining supercoiled... 2. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase....

  3. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ....com. ITR structure  ITRs are symmetrical 145-nucleotide sequences that flank the ends of the single stranded...Figure 2). Additionally, the ITR contains a 4-nucleotide Rep binding element (RBE) that serves as a binding...

  5. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...chromosomes and how these arrangements may affect nuclear processes. The latest of these visualization tools...the gRNA in living cells. Broccoli aptamers are nucleic acid sequences that bind to the small molecule,...

  6. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...contain other cellular molecules such as proteins or nucleic acids. This conjunction of vectors and exosome ...contain cellular macromolecules (e.g. proteins, nucleic acids) which possibly improve transduction properties...

  7. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    ...incorporation of a 3’-5’ exonuclease in the reaction mixture. The exonuclease removes mismatched 3’ overhangs...

  8. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...and has previously been used for multiplex Cas9-nuclease editing. Typically, a single array encoding the...editing, such as base editing, prime editing, and nuclease editing, in virtually any cell type or organisms...

  10. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...transfection process is not integrated into the nuclear genome, in the absence of plasmid replication, ... lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T antigen (...

  13. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...prevent them from being degraded by restriction endonucleases. A given prokaryote typically has genes encoding...groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same DNA sequence...

  16. Antibodies 101: Conjugation

    Type
    Blog Post
    ...we would be remiss not to at least mention. Oligonucleotides allow for sensitive detection of low abundance...multiplexing potential. You will find antibody-oligonucleotide conjugates used in assays like proximity ligation...

  17. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...underwent repair not from the single-stranded oligonucleotide (ssODN) template supplied by Ma et al., but...allele rather than the supplied single-stranded oligonucleotide (ssODN) template. Embryos also underwent editing...

  18. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...phosphorylated and red fluorescence can be found in the nucleus. For testing kinase inhibitors, psMEK1 and ERK ...downstream target ERK, the KTR remained in the nucleus. But if the inhibitor targeted upstream members...

  20. A Look at Addgene's QC Process

    Type
    Blog Post
    ...mutations, frame shifts, missing elements, and nucleotide indels or mismatches in significant plasmid elements... reference; and identifying mixed plasmid and nucleotide populations. All of these topics require additional...
Showing: 161 - 180 of 422 results