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We narrowed to 650 results for: CHI;

Showing: 241 - 270 of 650 results

  1. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...multiple genes to achieve a desired effect. This is commonly used in plant biology to achieve traits such as...organization schema MoClo can be broken down into three hierarchical levels: individual DNA parts, transcriptional...

  2. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...Chromoproteins also enhance corals’ resistance to mass bleaching, the loss of their symbiotic algae, during heat...cost and resources are limited or for bioart and teaching applications. Find a chromoprotein for your experiment...doi.org/10.1186/s13036-018-0100-0 Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N.,...

  3. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ... it has been challenging to introduce CRISPRi machinery into diverse bacteria species. Existing protocols...bacteria with a chromosomal copy of the RP4 transfer machinery mobilize these plasmids for transfer to recipient...contain a chromosomal copy of the RP4 transfer machinery, which mobilizes the Tn7 plasmids, and lack the...

  4. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus... https://doi.org/10.1096/fj.01-0995fje. Tan, E., Chin, C. S. H., Lim, Z. F. S., & Ng, S. K. (2021). HEK293...

  5. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...writing a grant, thinking about the next project, reaching out to other teams, expanding your network…. I...problem, I was able to resolve this issue - with coaching - before it became a major problem. But I also...always the case or you may not feel comfortable reaching out to your manager. This is when having a mentor...

  6. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ... instances of focused attention become magical teaching moments. The models function as thinking tools...that the resulting physical model is an effective teaching tool. You can read about Jacob’s initial reaction...BioMolecular Modeling web site to check out our hands-on teaching tools that are designed to make the molecular ...

  7. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ... functional groups: nucleases, modulators, and chimeric effectors. Nucleases generate a classic double-stranded...break, while modulators regulate transcription and chimeric efforts make a precise edit or insertion via a...distributed CRISPR plasmids. But modulators and chimeric effectors, made available in 2013 and 2016, respectively...

  8. Of Myc and Men

    Type
    Blog Post
    ... that this step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds...c-Myc protein and did not recognize the mouse or chicken homologs. By comparing the homologous sequences...Myc1-9E10 in the late 1990s (Fuchs, et al. 1997; Schiweck, et al. 1997) with the goal of making the antibody...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...concentration. Add supplements to the media You can achieve higher cell density using a nutrient-rich broth...plasmids may need to be grown for 20 hours or more to achieve maximum plasmid yield. The optimal growth time ...insufficient gas exchange will prevent your cultures from reaching the desired density. The volume of your flask ...

  10. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ... is valuable in many experimental settings. To achieve this, scientists use a multitude of techniques ...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...to plasmids, allowing you to spend less time researching plasmid basic features and spend more time developing...

  12. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...responsible for an autosomal recessive, severe, childhood-onset form of blindness name Leber’s Congenital... therapy restores vision in a canine model of childhood blindness. Nature Genetics 28, 92-95. PubMed PMID...PubMed Central PMCID: PMC5960719. 8. Trapani I and Auricchio A., 2018. Seeing the Light after 25 Years of Retinal...

  14. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...was derived from figure 3b in Chan et al.   To achieve the full diversity of colors AND sparse labeling...co-injected. The dose of this AAV can be adjusted to achieve sparse levels of labeling, without loss of color...virus, but the desired sparsity of labeling is achieved with lose doses of inducer virus. Additionally...

  15. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital PCR as a tool for...is common. Here are some steps you can take to achieve a clean NTC: Have a dedicated bench with a dedicated...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...while not widely known, plasmid assembly can be achieved in vivo using a bacterial recombination pathway...in a gene, while adding a GFP-tag (Figure 3). Achieving multiple modifications requires multiple recombination...DNA encoding plasmids, or promoters such as the chicken beta-actin promoter of the pHLsec vector. In this...

  17. CRISPR Antimicrobials

    Type
    Blog Post
    ... approaches. Major restaurant chains including Chipotle and Panera Bread have pledged to eliminate the...bacteria have poor non-homologous end joining machinery, so a CRISPR-induced double-stranded break (DSB...into the bacterial genome, and it carries CRISPR machinery targeting 1) the given resistance gene and 2) ...

  18. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    ...and the viral mRNAs are translated) by cellular machinery. NP encapsidates the negative sense, single-stranded.... These mRNAs are translated by cellular host machinery (as viral mRNAs would be in the course of viral...initial titration experiments might be helpful to achieve optimal results. It is important to note that this...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...a synonymous mutation can be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may... A, Santana-Quintero LV, Katneni U, Simonyan V, Kimchi-Sarfaty C (2017) A new and updated resource for... https://doi.org/10.1093/nar/28.1.292 Sauna ZE, Kimchi-Sarfaty C (2011) Understanding the contribution...

  21. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...leading to a positive signal. The same effect can be achieved for a fluorophore that relies on dimerization ...cleavage and an RNA aptamer that bound the compound malachite green (MG) to create a non-protein based visualization...of high throughput assays with microarrays and biochips. Ultimately, there are just some applications ...

  22. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...salaries). Postdocs (who are not in a primarily teaching role) come under this ruling, regardless of visa...on higher education, you can see that those in teaching roles (such as adjuncts) and graduate and undergraduate...already have salaries at or above $50,000. Boston Children’s Hospital is the only part of the Harvard group...

  23. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...projects. Almost all of my projects, have involved enriching conversations with R&D, Medical Affairs, and Commercial...challenges, quickly breaking them up into smaller achievable tasks, and then executing on them in a timely...situations, and personalities. Teams striving to achieve a lot of great output in a short amount of time...

  24. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter...relocation plans. They can be skills-based: if you're switching fields, you can use your cover letter to explain...greeting like “Dear Hiring Manager.” Highlight your achievements with specific examples. Instead of saying “I...

  25. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...native conditions, the less activation you can achieve using a CRISPRa. Your gene of interest might already...Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon...Zetsche B, Volz SE, Zhang F (2015) A split-Cas9 architecture for inducible genome editing and transcription...

  26. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...Improvements in our discipline for annotation and archiving of data are likely to improve research. What is...common file types? And how long should data be archived? Simplifying data access Some of the above mentioned...data repositories emerge that allow efficient searching and extraction of information. Looking a little...

  27. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ... in your host organism. Because transcription machinery differs between cell types or organisms, promoters...from beta actin gene Constitutive  Ubiquitous. Chicken version is commonly used in promoter hybrids. ... promoter Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice...

  28. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...Matthew J., et al. "Toxic protein expression in Escherichia coli using a rhamnose-based tightly regulated...enables simplified chromosomal gene replacement in Escherichia coli." Appl. Environ. Microbiol. 81.15 (2015)...Recombineering) system for genome editing in Escherichia coli." Scientific reports 5 (2015): 15096. PubMed...

  29. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ....e. those things that will be most useful for achieving your goals. However, you should have a good mixture... up with too many tasks. If you’re constantly switching between different projects, you might not be able...thinking about when or how long it would take to achieve these goals. I have far too many projects to operate...

  30. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...highlight the specific modifications to CRISPR machinery that allow for the use of CRISPR in plants, and...in question. In plant cells, gRNA expression is achieved by placing the gRNA downstream of a plant-specific...Chloroplast-selective gene delivery and expression in planta using chitosan-complexed single-walled carbon nanotube carriers...
Showing: 241 - 270 of 650 results