We narrowed to 877 results for: HA

...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer...prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at about ...

...studying the genetic underpinnings of these processes has been complicated by the lack of precise genetic tools...Simplified in vivo delivery using SPH mice CRISPRa has largely been limited to in vitro settings due to ...can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach... or coding regions. Typically, each gene isoform has a distinct transcriptional start site. Because CRISPRa...

... is easy to use, functional in most species, and has many applications. That said, SpCas9 is not the only...SpCas9. Cas13a (previously referred to as C2c2), has several unique properties that further expand the...single A, U, or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as...This system, dubbed SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus,...

...plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all of...they were prior to amplification. And as anyone who has worked with individual plasmids knows, some plasmids...size and backbone.  Verification Once your library has been amplified, you'll need to verify your library...transduction conditions, you’re ready to transduce! Addgene has a transduction protocol you can follow and a troubleshooting...

... Cas11-like protein, and a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature...kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed PASTE, which can “drag and drop” kilobases...PASTE is more specific and efficient than HITI and has higher purity of insertion than both HITI and HDR...therapeutic applications. The potential of CRISPR has yet to fully taken advantage of, but exciting new...

...that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids... based on 100 structure-guided spike designs and has improved protein yields and stability. This stabilized...virus spreads around the world, the spike protein has had many opportunities to evolve. Several variants... to accelerate research through sharing reagents has never been so important.     References and ...

...CRISPR has become a household name, the search for smaller genome editors is heating up and TnpB has found...planning on lounging poolside or swimming laps, Addgene has pooled libraries for any summer project. While many...neurons. Created with BioRender.com. Addgene has tested these capsid plasmids in vitro and uses them...

...off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (Delivery to Intended...on-target delivery with various delivery modalities and has the potential to reduce negative side effects. This... assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection... collection since we began distribution and has been verified to perform well in standard IHC and western...

...means the bacterium is not naturally resistant but has become resistant somehow overtime. One fantastic ...on a plate! Antibiotic resistance can arise, and has arisen, for many different reasons. Exposure to antibacterial...presence of a known concentration of antibiotic that has been impregnated into a paper disk, and observing...resistance; it’s a natural process of life and one which has allowed bacteria to evolve and survive for millennia...

...world, is not inert. In all seriousness, EGFP/GFP has a real nontrivial propensity to noncovalently dimerize...artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet many investigators...dimerizing protein of interest fused to a dimerizing FP has potential to form large stable polymers of dimers... monomerized with an A206K mutation [2]. Addgene has many of these constructs and they are designated ...

...joined Addgene's Advisory Board.  Addgene: Your lab has worked extensively with enChIP systems. Can you describe...advantages? Fujii: In the last several years, my lab has been working on development of technologies for biochemical... systems to Addgene in the near future. Addgene: Has your lab been working on any other technologies that...plasmids. The merit of depositing plasmids to Addgene has been spectacular. We've got more than one hundred...

... to monitor cellular perturbations to how CRISPR has made it much easier to generate mouse models.   ...data from the Kim and Joung Labs showing that Cpf1 has lower off-target editing and similar on-target editing...

...proteins (GFP and YFP) and protein tags (c-MYC and HA). We have also created the Monoclonal Antibody Research...community. Thanks to Our Guest Blogger!   David Soll has been the Director of the Developmental Studies Hybridoma...

...science and our plasmid collection (what science has been hot this year and how do you creatively turn...procrastinating choosing their costume?  As our organization has grown, we continue to have an amazing turnout for...

...Adeno-associated virus (AAV) has many features which make it a great viral vector, but its packaging ... Overlapping  Overview: The overlapping strategy has a region of homology, typically ~400-1400 bp long...Drawbacks:  Generally an overlapping split vector has equal or higher transgene expression when compared...split AAV method While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing...efficiency of transgene expression from split vectors has been inconsistent. A greater understanding of basic...

...Your new antibody has arrived, hooray! Before you jump straight into the exciting new experiments that...good idea to pause and make sure that your antibody has been appropriately validated so that you can have...application. The goal is to show that your antibody has been validated using at least one of these strategies...is binding to something unexpected. This approach has high specificity, but you need to be aware of any...found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9. You check the mutation...

... S. University of Baroda, India  Devarshi Gajjar has 10 years experience in the field of bacterial and...biomedicine and astrobiology. During the last 11 years he has studied various aspects extreme life on earth including...educational resources for scientists globally, Addgene has partnered with various community organizations and...

...in previous animal studies suggested that fasting has a positive effect on cancer treatment (Ref 1). As... mutations and clinical responses. This platform has the potential to become an open-source pipeline and...of the plasmids on her poster is pTomo-Ras which has been deposited at Addgene was used to produce the...

...useful research materials and information. Addgene has been successful in helping thousands of sharing scientists...depositors and partners. The Addgene Scientist Team has been developing expertise in answering questions ...from plasmids in the repository. The Outreach Team has been making sure scientists know about the new initiative...

... capsid? A: It is our understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore...but reproducible success remains elusive.  There has also been a lot of effort in immunomodulation or ...AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms.  ...

...Addgene has recently announced the launch of an open-access recombinant antibody resource called NABOR...Detailed figure legend here.     The Trimmer Lab has continued to work to make the sequences of monoclonal...collection and make them publicly available. The lab has also expanded their recombinant affinity reagent ...

...partnership was fruitful and a first version of the study has just been uploaded to the preprint repository BioRxiv... platform; while they are a minority, their help has been essential to push certain projects. The idea...year on, our biggest lesson running the platform has been the realization that there is a great untapped...

...makes a good mentoring program. If your organization has a mentoring program, consider tapping into it and... have going on locally. Your university probably has an alumni directory. Alumni who enter their information... own peer mentoring circle. This type of program has many advantages and I am seeing a lot of success ...

...Request, an expansion of our viral vectors service, has officially launched! As of today, you can request... on.  Expanding our viral vector service Addgene has offered in-stock viral vectors since 2016, with the...

...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...anytime. The CSV is a rich source of information and has data on the date, material, requesting PI & organization...

...continue their careers in the United States. There has been a growing shift away from employers sponsoring...that the researcher is working under in the lab or has won, awards, media reports, and membership in groups...that you are a classically trained ballerina who has performed at Lincoln Center. Your case should only...only support tenure track professors. Casseus Law has seen that research institutions are willing to sponsor... your current research goal is and why your work has impacted your field (i.e. finding vaccines for deadly...The platinum standard for an expert is someone who has cited your work. Immediate steps that you can take...

...changes, and the addition of protein tags. It also has the flexibility to modify the E. coli chromosome,...recombineering system The lambda red recombineering system has three components (Figure 1): 1) Exo, 2) Beta, and... in the center of the sequence. Since lambda red has a higher recombination frequency when the lagging...activating MMR: 1) use a strain of bacteria that has key MMR proteins knocked out or 2) specially design...recombinant clones If a antibiotic resistance gene has been inserted, recombinants can first be selected...

... Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer specific...databases annotate the TSS in different ways, and it has been shown that the FANTOM database, which relies...mismatches that can nevertheless still lead to activity has been established, and can be used to identify sites...Perturbation Platform at the Broad Institute and has worked with many Addgenies to help improve the understanding...e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base editing: precision chemistry ...

...trimers, tetramers). Since then, multimerization has been observed to occur in many different kinds of...sequences. In some specific cases, multimerization has been observed to occur more frequently in plasmids... Ausubel, 1976). In fact, if your plasmid sample has a mix of different-sized multimers, you may even ...~3.6 kb and ~1.1 kb (lane 3). Uncut DNA (lane 2) has a band at <3 kb, which corresponds to the supercoiled...multimers is capillary gel electrophoresis (CGE), which has been used in plasmid vaccine and gene therapy development...

... flow rate into the pipette tip. Once the liquid has finished drawing into the pipette tip, lift the pipette...

...kD TurboID, which has 15 mutations compared to BirA and the 28kD miniTurbo, which has the N-terminus domain...proteins in minutes rather than hours like BioID. APEX has allowed scientists to identify the proteomic map ...BioID was developed in 2012, proximity labelling has become a powerful technique for studying protein-...

...The Rett Syndrome Research Trust (RSRT) has an ambitious goal of curing Rett Syndrome and Addgene is ....  As a non-degenerative, monogenic disease that has shown reversibility of symptoms in animal models,...neuronal subtypes of the hippocampus. This technology has formed the basis of the Rett syndrome program being....1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen TL, Rajewsky K, Chu VT (2019) ...

...within the human genome. Another variant, xCas9 3.7 has 7 mutations found in the REC2, REC3, and PAM interacting...CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically increase the number...additional targeting flexibility. Cas13a (formerly C2c2) has been adapted for targeted RNA cleavage in mammalian... MH, Tang W, Chen L, Sun N, Zeina CM, Gao X, Rees HA, Lin Z, Liu DR (2018) Evolved Cas9 variants with ...

...epitope (GKPIPNPLLGLDST) has been used as a tag since 1992 (Hanke et al., 1992), and has proven itself a versatile...like PROTAC targeted protein degraders. But CRBN has been difficult to study in vitro, requiring either...Cas9 system By Aryana Khosravani Deborah Hung’s lab has developed a novel dual-plasmid CRISPR/Cas9 system...

...optical pooled CRISPR screening, Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled... stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future...conferred several new improvements. The new variant has increased signal-to-noise ratio and better ability...limitations of traditional AAVs, the Becirovic Lab has developed REVeRT, for gene delivery using reconstitution...

...as a scientist has been building your creativity all along. In my own career, my team has used these techniques...and instead building an environment where everyone has their ideas heard.  So how can we assure that all...continue advocating for your idea. Our product team has implemented a policy of never rejecting an idea simply...

...right corner.      Figure 2: This flow plot has two populations that are B+ and one population that...Figure 3 above, until the population of interest has been successfully identified and isolated.  Following...from Boix et al., 2018, uses quadrants to gate and has numbers representing the percentage of cells in each...2017). Neonatal immune response to rhinovirus A16 has diminished dendritic cell function and increased ...

...never went away. While he has frequent bouts with Impostor Syndrome, Joe has found his people through ... match people within similar fields (ASM Microbe has eight tracks), something we didn’t have enough people...notebook paper, despite the fact that it was gray. He has worked in molecular and microbiology for the last...

... undermine critical research infrastructure that has taken decades to establish.These funding decisions...Together Throughout history, the scientific community has demonstrated remarkable resilience and creativity...

...time in grad school. As you'll learn, grad school has its ups and downs for everyone but you can come out...their grad student communities 5:59 - 8:16 : What has made you unhappy in grad school? As part of this ...