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We narrowed to 801 results for: MAK

Showing: 321 - 360 of 801 results

  1. Western Blot

    Type
    Protocol
    ...lid of the device. Select the desired method and make sure the parameters are correct. Transfer for 5–...

  2. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...differentially expressed genes can be the next stage in making sense of such data. These targeted screens can ...you have your list of genes, Green Listed can help make the gRNA design process quick and easy. Green Listed...information that you have input in the web interface, making it convenient for you to later identify how you...letting us use their reference libraries thereby making it possible to put together this software. To me...

  3. Addgene at SfN23!

    Type
    Blog Post
    ...you go!  Tips to Make the Most of a Scientific Conference Poster Session TipsHow to Make Friends and Meet...Specifically, Jo, Brook, and Angela are co-leading "Making a Greater Impact Using Clear and Accessible Scientific...

  4. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ...taking care at each step, but some purification kits make it easier than others to prevent quality issues....miniprep kits! Larger kits generally take longer.) Make sure to take a look at the manufacturer’s kit pages...select a kit. So...which kit should you pick? To make it easier to compare kits at a quick glance, Table...budget is an important consideration, you do want to make sure you’re getting the best bang for your buck....

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...target bases without breaking the DNA backbone, making them efficient and precise tools for altering DNA...the core ring structure intact, allowing them to make 4 out of the possible 12 base-to-base conversions...were engineering an ABE to reduce its tendency to make off-target edits to RNA. Like other ABEs, the editor...mammalian cells Researchers in the Xueli Zhang lab were making similar observations around the same time. (The...

  6. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

    Type
    Blog Post
    ...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com... wrapped gifts using life science catalog pages. Make your WFH space feel a little more festive this holiday... entering in (if you don’t include this, we will make our best guess). Follow @Addgene, use the hashtag...

  7. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    ...experiments. Best practices!). Making the find plasmids badge appear So how do you make this magical Addgene badge...favorite topic. If you have any other cool tricks to make PubMed browsing even easier and more useful, let...

  8. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    ...recent blog post, we looked at how long it takes to make one plasmid and how much time Addgene has saved ...researchers. Assuming 5 hours of hands-on time to make each plasmid, we would have saved 5 million hours...will be moving into a bigger space so that we can make an even bigger impact - we are always on the lookout...

  9. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular...additional questions. We’re happy to see scientists making their own cells to save money and create the tools...Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids...

  10. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your... and the small difference in protein expression makes confirmation of antibody selectivity even more challenging...used has endogenous levels of expression, this can make the results more difficult to interpret, and a knockout...Antibodies. The Only Good Antibodies community works to make best practices in research antibody selection and...

  11. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...Neuroscience meeting in Washington, D.C., we must be making a dent in the global coffee supply… My back-of-...we've spoken with are excited and passionate about making such tools open-access. Though it is time for me..., remember there are only 2 days left for you to make the most of this great opportunity – so if you’re...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...capacity (8-10 kb) than other retroviral vectors. This makes it possible to use a polycistronic cassette that...Another challenge is that multiple transfection makes it difficult to control the dose of plasmid the ... pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and... and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent stem cells: reprogramming à...

  13. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...is being an overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping forward...in a life sciences career but life has a way of making unexpected decisions. As someone who was not necessarily...additional person each day. It felt like I could actually make connections with people. These were some of the ...

  14. Reflections and Looking Ahead

    Type
    Blog Post
    ...story. This year, we made significant strides in making science more accessible and reliable. We continue...experimental results. We also redesigned our website to make it easier to find what you need and to bring it ... every breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank...

  15. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...control of an otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled...paired with any Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1...other viral vector tools in the years to come. We’ll make many more viral vector tools and resources available...

  16. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...expression vector and attached a DNA barcode to each to make the library usable for pooled screens. Applications...different contexts.” It’s also possible you could make use of these plasmids in living animals, to explore... that’s thrilling to us,” Wood says. “We want to make these available as widely as possible.” The researchers...

  17. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...this interest in the ways science is shared and in making it easier to share, we take special note as the...Way to Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As... As we prepare to ring in the New Year, let's make a toast to open science. And, please, share your favorite...

  18. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ... the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative...derivative to conduct subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs)...the unexpectedly photoconvertible GFP proteins to make a new transgenic line could avoid problems in localization...

  19. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...partners and Tech Transfer Offices at universities to make COVID-19 research-related plasmids available more...Find plasmids available to industry scientists To make plasmid sharing more open for academic and industry... deposited plasmids at Addgene and would like to make them available to industry, please reach out at ...

  20. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ... by making them brighter, more photostable, smaller, and less toxic to cells. They’d like to make near-infrared... muscles work inside a living animal. For those making use of these probes, he says, when in doubt, read...

  21. Fostering communication in remote collaborations

    Type
    Blog Post
    ...best way to keep everyone on the same page is to make communication a non-negotiable part of your collaboration...Luckily, there are techniques and tools that helped make my experience run smoothly and can help other teams...meetings can be tricky, and it takes extra effort to make sure everyone in the meeting has an equal opportunity...

  22. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...toxicity or high off target effects, both of which make it a non-ideal drug. If a drug selectively kills...added QC is where CRISPR can save time and money to make sure quality candidates are selected.      ...Additional resources on the Addgene blog CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to Edit...

  23. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...because I knew it would make me a better writer, but also because I knew it would make me a better scientist...mates, you add the individual figures and results to make the final product with an interesting introduction...

  24. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ... mechanisms for feedback (more on this below). Make sure to give positive feedback when it is merited...some people find this hard so get used to it by making it a concrete goal to say “thank you, job well ...unnecessarily big issue for you and for the employee. Make sure to focus on the action, not the person - intent...

  25. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    ... of the features you may want to consider as you make your choice. The first and most important thing ...vector that you will need. You will also have to make sure that your plasmid has been incorporated into... applications used to study gene function. From making transgenic animals, gene targeting and genome modifications...

  26. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ... of bacterial genomes utilize this system (4). Makarova et al. grouped the systems into three distinct...Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 Dec; ...1709–1712 (2007). CRISPRdb. Date: 2013-09-18. Makarova K, Haft DH, Barrangou R, Brouns SJJ, Charpentier...

  27. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the...sequence Inverted terminal repeats (ITRs) are what makes an AAV transfer plasmid an AAV transfer plasmid....

  28. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...development, vaccine development, and assay technologies makes her a perfect fit for Addgene. She was Head of Quality...around the world who would otherwise have difficulty making or getting these reagents is profound.” Frank Stegmeier...we’ve built create inefficiencies that ultimately make innovation and science slow,” Vandenberghe says....

  30. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ... A good rule of thumb is to find the pieces that make you think, “Oh, that’s interesting! How did you ...in with something that sparks their interest and makes them want to know more. This could be by outright...needs to capture your audience’s attention enough to make them decide to read (or listen or watch) further...

  31. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    ...spatio-temporal regulation, and is difficult to reverse, making it unsuitable for some types of experiments. (To...arabinose, requires many wash steps to remove arabinose making it difficult to regulate quickly. Arabinose also...Khammash M, Di Ventura B (2021) Engineering AraC to make it responsive to light instead of arabinose. Nat...

  32. What the HEK?

    Type
    Blog Post
    ... four. No Y chromosome is present in this line, making it genetically female. HEKs are generally cultured...properties. HEK-293 cells are tumorigenic, which makes them a candidate cell line model for cancer-specific...reproducible results. It’s also widely available, which can make for a seamless transition of biological tools to...

  33. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...screening experiments. Get the full details on how to make these libraries from the post and the associated...Editing CRISPR has revolutionized how researchers make knockouts and other types of genome edits, but, ...depositors and guest writers. Your contributions make Addgene and the Addgene Blog useful resources for...

  35. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...resolution functional tracking of T lymphocytes, too, making them a versatile tool for application in both neuroscience...element-binding protein (CREB) in living cells. “Now we are making this really good,” Griesbeck said. “We know CREB... but not the other). “It’s quite a robust way to make a FRET sensor with a large dynamic range,” he says...

  36. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands on While...University of Barcelona wanted to develop strategies to make our classes more practical and hands-on. Students...interactive setting. The problem is…how can you make a “hands-on” lesson on genetic manipulation of human...

  37. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...greater familiarity with scientific methodology makes it easier to spot even minor weakness or limitations...Zealand’s multiple Covid-19 lockdowns, I held mask-making workshops in my community. I brought along printed...run workshops where I showed people how they can make reusable cloth menstrual pads while we talked about...

  38. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...researchers to utilize and develop these tools by making their own fluorescent protein technologies widely...off with light.  Xin hypothesized that she could make enzymes controllable by tethering them to Dronpa...communication skills throughout her training and wants to make her work understandable to people both inside and...

  40. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ... of making science easier and more transparent. That’s largely my job [at the Broad] too… to make science...help guide and promote Addgene in its mission to make research easier. Addgene is excited to announce ...
Showing: 321 - 360 of 801 results