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  1. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...questions concern great scientific problems, but the technology may not exist to answer the question or it might...received his PhD in Molecular Microbiology and Immunology from Oregon Health Sciences University, did his...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...proteins with small molecules in vivo. Nature Biotechnology, 21(1), Article 1. https://doi.org/10.1038/nbt765... V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis. ACS Chemical...

  4. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...Sullender M, Ebert BL, Xavier RJ, Root DE. Nat Biotechnol. 2014 Sep 3. doi: 10.1038/nbt.3026. 10.1038/nbt...Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed PMID...

  6. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... label it with finesse using tRNA suppression technology (Fig. 1) (Dumas, Lercher, Spicer, & Davis, 2015...References 1. Degorce, François, et al. "HTRF: a technology tailored for drug discovery–a review of theoretical...

  7. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red and Cas9, which...for highly specific genome editing.”  Nature Biotechnology 32(6) (2014): 569-576. PubMed PMID: 24770325...

  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...have been making major improvements to CRISPR technology since its initial applications for genome engineering...CRISPR-Cas9 for optogenetic genome editing. Nat Biotechnol 33:755–760 . https://doi.org/10.1038/nbt.3245...

  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ... PubMed PMID: 28585549 Kim Y, et al. Nature Biotechnology. 2017. PubMed PMID: 28191901 Listen to Our ...vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library targets 289 genes...

  11. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...membrane, you can dry the membrane, wet it with 20% methanol, and place over a light box to visualize proteins...the bubbles in the membrane sandwich Ensure methanol concentration in transfer buffer is < 20% ...

  23. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ... understanding and application of CRISPR/Cas9 technology are coming along at a fast and furious pace. ...

  24. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial expression ...

  28. Let There Be LITE Plasmids

    Type
    Blog Post
    ...including Zhang and Mark Brigham have taken the technology to another level with the addition of two light-sensitive...

  30. Editor's Choice, September 2016

    Type
    Blog Post
    ...can find the rest of our September posts in chronological order here: Four Things I Learned at the 10...

  37. Science as Art

    Type
    Blog Post
    ...Richards’ work is held by The Museum of Jurassic Technology in LA, where it is currently on display.   Many...

  40. What's New in CRISPR - March 2020

    Type
    Blog Post
    ...relative to BE4max.  Read the paper in Nature Biotechnology Find the base editor plasmids CRISPRi in Caulobacter...
Showing: 321 - 360 of 545 results