...addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal...source project that is creating the “first virtual organism in a computer.” This project has produced various...Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open resources described...highlight a few toolkits that we have available at Addgene that were discussed primarily at the workshops ...interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...resources about C. elegans and the plasmids we have at Addgene check out our worm expression resources page. This...PMCID: PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR...

...maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid Guide     ...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in..., enter the common or scientific name into the Organism box. This small piece of information can significantly... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...NCBI BLAST website. Additional Resources on the Addgene Blog: Plasmids 101: How to Verify Your Plasmid...

...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with... community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation...27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches...e15272. PubMed PMID: 27090089. Resources on the Addgene Blog Read other Synthetic Biology Posts Listen...Your Next Experiment Deposit Your Plasmids with Addgene ...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...

... at a professional organization to expand your skill set Resources on the Addgene website Become of ...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific...of a member of the Addgene depositor community ...

...types even when systemically administered in an organism. These features make AAV ideal for human therapies...2023). PubMed PMID: 36899921. Resources on the Addgene Blog New to Virus Work? Check out Our Primer for... Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik. Didem Goz Ayturk...Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All...

...you can accomplish this by employing a neat and organized format.  Columns. You can choose from either ...make it your own! Additional resources on the Addgene blog Check out this blog post on navigating the...

...field has its own framework: a particular way of organizing and connecting information and a logic to the...accessible to your audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...

...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse... a mouse strain. Additional resources on the Addgene blog Find our CRISPR blog posts Read blog posts... genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering...

...learning.” Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since...work at Addgene. I posed this question to all nine of my interviewees. Fabienne Furt, Addgene’s Associate...resources on the Addgene blog Browse our science career blog posts Download Addgene's science career guide...stay in science.” Addgenies Beth Kenkel and Shannon Rinaldi in the Addgene lab! Read their Instagram... guide Resources on Addgene.org Learn more about working at Addgene ...Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member...go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community...

...applications for funding from charities, commercial organizations, or universities may have someone senior from... why they should give you money. Check out the Addgene Blog for Some Ways to Improve Your Science Communication...multiplex assays.   Additional Resources on the Addgene Blog Learn How to Become a Grant Writing Professional...

...Heckler. Additional Resources on the Addgene Blog Get Addgene's Take Aways from the FoR 2015 Symposium...Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future...

...frequently used species due to its use as a model organism in genetics and biochemistry. When using S. cerevisiae... use. Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD from Northeastern University...proteins in plant root exudates Resources on the Addgene Blog Learn about inducible promoters Learn about...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...

...promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017...capturing information about spatial and temporal organization. The design use to detect PKA activity can be...sREACH. Find the FLIM-FRET acceptors here. See Addgene's FRET reference page for many other common FRET...  Various Addgenies...

...PBS Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein...function, and can have a specialized role within an organism. Mechanisms of epigenetic modifications The main...

...Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they...Everybody's favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning ...elements, which has not been available for plants. Addgene: Will the MoClo tools work in any plant? Patron... it a good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those looking...start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working on for plants...interaction work and we’ve gotten some tools back from Addgene recently to explore their use in plants, including...technologies in the near future. Science on Twitter Addgene: You are active on twitter @nicolabiologist, where...

...including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate... K. 1993. PubMed PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ...

...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...University Medical Center are now available at Addgene along with a description of their experimental ...The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6...Maddalo et al., 2014). These tools are available at Addgene and could be useful to labs wishing to create this...including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from...technology guides, FAQs, and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT ....1089/hum.2015.087 Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome...

...yielding promising results in the laboratory, and Addgene is accelerating this research by helping to make...proliferation. Hence it is imperative to the health of an organism that both classes of genes function normally to...possible. Connecting the research community - Addgene's cancer collection pages Scientists are making...latest resources? In response to this challenge, Addgene has launched an initiative to curate our collection...direct the investigator to a list of all available Addgene plasmids he or she can use to study it (as seen...section below. Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing...Institute: Ras Pathway v2.0 Page Resources on the Addgene Blog Tackling Cancers' Drug Resistance with a ...

...From the beginning, one of Addgene’s goals was to ensure the scientific community obtained high-quality...the years to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions...will describe the importance of bioinformatics at Addgene and how our search for solutions led us to a partnership...bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow...than half of these sequences were generated by Addgene as part of our QC process. Automation and continuous...pipelining In many use-cases, including ours at Addgene, the overall purpose of a data pipeline is to perform... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...

... do not necessarily represent the views of Addgene. Addgene performs Sanger sequencing on select regions... success of CRISPR-Cas9 across multiple model organisms (3) lies in the versatility of its delivery methods...Twitter - Tweet suggestions and tips with #NgAgo The Addgene Blog - Leave suggestions and tips in the comments...PubMed Central PMCID: PMC4343198. Resources on the Addgene Blog Learn More about NgAgo Enhance CRISPR Targeting...comments section below or contact blog@addgene.org if you'd like to write a more detailed post It may take...Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org Request NgAgo Find CRISPR Resources Catch Up...

...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn...provide an on-topic answer by creating time to organize your thoughts. It’s likely you won’t know every... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...

...directly from A. Victoria and expressed in any organism while still maintaining fluorescence. The protein...where the gene is expressed. Click here to view Addgene’s collection of empty backbones for constructing... such as FRET, calmodulin, and others. Review Addgene's collection of fluorescent biosensors here. Optogenetics...OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs ...microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim...

... of your gene’s functional biology in a living organism. This is the first post in a three-part series...own mutant flies. Find Drosophila Resources at Addgene The birds and the bees when it comes to fruit ...basic knowledge to start working with flies. Like Addgene, the Drosophila community is highly sharing-oriented... a “pound symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

...their presence. They are genetically modified organisms, and GMOs have a bad reputation. The public is...Russell Terriers. More SynBio Reading Read more Addgene blog posts about synthetic biology ...

...of tube for each plasmid we have at Addgene. Each sample at Addgene has four layers of location which can...pages! Resources Avaliable on the Addgene Blog Read more Inside Addgene Posts! Learn all about Plasmids ...Every plasmid sample enters Addgene the same way. A package is delivered by a mail courier and then the... a microcentrifuge tube. Once a sample enters Addgene’s lab, a series of events is triggered. Each step...fluctuates throughout the year (see graph below). Addgene scientists inspect each sample and make sure they...takes over and prepares the sample for entry into Addgene’s repository. For long-term storage in our facilities...location There are many different types of samples at Addgene - DNA sent from depositors, bacterial glycerol ...

...window) . Addgene Website & Blog References Website CRISPR plasmids and resources at Addgene CRISPR 101...cell lines Viral service at Addgene Viral plasmids and resources at Addgene Blog CRISPR topic page How ...Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. Science...knock out target genes in various cell types and organisms, but modifications to various Cas enzymes have...You Use a CRISPR Library? CRISPR libraries from Addgene are available in two formats: as liquid DNA, or...be added to detect the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar...located. The majority of the CRISPR plasmids in Addgene’s collection are from S. pyogenes unless otherwise...

... Joanne Kamens Resources at Addgene.org Find Open Positions at Addgene ...training in a variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting...Chemical Society (ACS) are just 2 examples of organizations that hold large, annual conferences. Gordon ...that person before ever asking them for work. Addgene’s very own Joanne Kamens, a strong advocate for ...Scientists on docjobs.com Additional Resources on the Addgene Blog Check Out Career Topic Page Tips on Writing...

...Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease...Glazebrook, 2002). Find plant plasmid resources at Addgene  Choosing an expression system Choosing an appropriate...21 September 2018). Additional resources on the Addgene blog Engineering the plant genome using CRISPR...resource page Deposit arabidopsis plasmids with Addgene ...Using arabidopsis in the classroom Resources on Addgene.org Find plant plasmids Visit our plant resource...

...CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-Cas system they block can...10.1126/sciadv.1701620 Additional Resources on the Addgene Blog First time CRISPR user? Check out this post...Optogenetic Control of CRISPR   Resources on Addgene.org Find CRISPR Plasmids for Your Research Interested...

...been an invaluable resource for studying the organization and function of cellular systems. FPs have been...PMCID: PMC4889673. Additional Resources on the Addgene Blog Learn about Fluorescent Protein Timers Check...engineering FP fusions associated with cellular organelles, scientists have been able to study many cellular...Use Rosella to Monitor Autophagy Resources on Addgene.org Check out the SapTrap Kit Page Find Other Fluorescent...

...connected to the neck. Shortly after, we learned about organs and the jobs they perform. This became foundational...Pubmed PMID 17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS...Gene Expression with FLEx Vectors Resources on Addgene.org AAV Vector for TVA Expression AAV for Rabies...

...proximity labeling experiments in cells, tissues, and organisms. Lee, S.Y., Cheah, J.S., et al. (2023). Engineered...43R] has been one of the hottest antibodies in Addgene’s collection since we began distribution and has...[K28/43R] under the Application section of the Addgene antibody page, and learn more about the whole-brain...

...doi.org/10.1093/mutage/gei016I Resources on Addgene.org Addgene’s Basic Molecular Biology Protocols Over-...Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies...Antibiotic Plating Plasmids 101 eBook Resources on the Addgene blog Plasmids 101: Antibiotic Resistance Plasmids...

...Vectors 101: Viral Applications Resources on addgene.org Addgene's Lentiviral Guide Most popular lentiviral...Resources and References More resources on the Addgene blog Viral Vectors 101: Viral Vector Elements ...Telesnitsky, A. (2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus... This blog was written by Addgenie Brian O'Neill, with thanks to Addgenie Rebecca Hutcheson for her assistance...

...Your Lentivirus Preps More resources on Addgene.org Addgene's virus protocols Viral Plasmids and Resources...types, spanning both prokaryotic and eukaryotic organisms. Typically, what system is used is dictated by...Resources and references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV...

..., which connect the heart, lung, gut, and other organs to the CNS, are also an important target for gene...Central PMCID: 3293995. Additional Resources on the Addgene Blog Important considerations when using AAVs ...AAV, and much more! Find ready-to-use AAV from Addgene here or check out these protocols if you want to...Viral Vector Blog Posts Additional Resources on Addgene.org Search for AAV Vectors to use with your research...

...Resources on Addgene.org Browse our Fluorescent Protein Collection Find Plasmid Kits at Addgene Find Plasmid...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The...and BamHI EcoRI Additional Resources on the Addgene Blog Read about Gibson Assembly Learn About Other... you use MXS Chaining by emailing us at blog@addgene.org. Table 3: Chaining Based Cloning Methods ...

...Resume Writing Resources on Addgene.org Check Out Career Opportunities at Addgene ...pharmaceutical companies, nonprofit or global health organizations, investment and equity research firms, biotech...it's impact on human health.     Resources on the Addgene Blog Learn How to Experiment with New Careers ...

... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...pair. Find BiFC plasmids for your experiment at Addgene! How to Design a BiFC Experiment Much like FRET.../10.2144/000113943 Additional resources on the Addgene blog Browse our Fluorescent Protein 101 blog posts...covering an introduction to FRET Resources on Addgene.org Find fluorescent protein plasmids and resources...

...including the age of the model organism, injection site, and titer. Addgene’s Viral Vector Service Besides...in the Addgene repository each year from 2016–2023. B) AAV preps distributed per year by Addgene from 2016...available at Addgene from 2016–2023 by type of tool. B) AAV preps distributed by Addgene per year from...As part of Addgene’s 20th anniversary celebration, we’ve been finding and sharing stories unique to our... our repository and resources. As editor of Addgene’s eBooks — including the recently released Viral Vectors...quality of the prep. This was a large factor in Addgene’s decision to launch our viral vector service in...able to order high quality viral vectors from the Addgene repository can save researchers significant time...