We narrowed to 539 results for: try;

...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially...

...applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC... a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7. https://doi.org/10.1038/s41557-023-...

...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...

...presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J. E., & Gomes, A. V., “Stain-Free...control to β-actin for Western blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j....

...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...

...Biotinylated proteins were isolated, identified by mass spectrometry, and classified by source cell based on single-cell...widefield imaging, two-photon microscopy, and fiber photometry. Plus, both designs offer promising templates...

...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov &...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology...

... blots, ELISAs, immunoprecipitation, and flow cytometry. The better these tools are, the better the reproducibility...cells. This enables direct screening via flow cytometry for improved binding under defined environmental...

..., label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques...-based Photoactivatable Transcription System. Chemistry & Biology 22:169–174 . https://doi.org/10.1016...

...detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens in cells...

...via a proprietary BP clonase, creating an entry clone. The entry clone now has recombined attL sites flanking...made an entry vector containing your DNA of interest (or obtained one of the thousands of entry vectors...collection contains thousands of Gateway donor , Gateway entry , and Gateway destination vectors with different...reducing ligation efficiency. TOPO-ready Gateway entry plasmids are also available, allowing for rapid ...

... Transfection for Recombinant Antibodies Immunocytochemistry Protocol Western Blot Protocol Introduction...

...acids. Plasmids and Recombinant DNA Technology Chemistry techniques enable the isolation and purification...select or sort the cells by visualization or flow cytometry. Primer Binding Site A short, single-stranded .... Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. Empty Backbones...UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG Tyrosine Tyr Y UAU, UAC Valine Val ...

.... If you’re looking to design your first flow cytometry experiment, “Chapter 7: Sorting” is there to help...

...McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila WittTips for Getting a Faculty ...

...metabolic operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Constitutive Hybrid... The trp operon involved in the production of tryptophan is an example of a positively-controlled operon...

...Differentiation Antigen Workshops Immune cells Flow cytometry (potentially others) Data repository From ...

...one of the best of the best students in his home country. He was selected as one of 600 scholars among 100,000...

... seeking and expanding our collaborations with industry and public and private partners to create additional...

...clue: differences in the kynurenine pathway of tryptophan degradation. It turns out that activation of ...

...reagents available to scientists in both academia and industry. This year, we also launched the cloning grade...

...was recognized in 2008 when the Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for...

...Individual Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer...

...for another part of my job, I travel around the country and set up meetings with PIs to discuss the benefits...

...Needle sticks and ripped gloves are common points of entry. Biosafety should always be considered with respect...Blood-brain barrier (BBB) Layer of cells that prevent the entry of toxins and pathogens from the blood to the CNS...packaging cell line). Infection The natural process of entry and multiplication of a pathogen, like viruses or...

... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...

...USA); 3–4 days (international; varies widely by country and/or distributor.)  You should plan to receive...

...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

...often mean that scientists who live across the country or around the world from each other are collaborating...

...occasional TikTok. I was also responsible for typical entry level tasks such as stuffing new depositor welcome...

...names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

...term though, she did a one year postdoc in the Biochemistry Department at Iowa, but kept up a freelance ...

...an application support/sales representative in industry or a postdoc in academia, but knew that, without...

... Society, or maybe you think about a local food pantry or community arts organization. Many people are...

...finding a job. Many people in science will work in industry or take on collaborative roles in academia, and...

...organizations such as the Center for Science and Industry (COSI) and the Franklin Park Conservatory. Do ...

...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

...transfected with three different vectors and flow cytometry is used to sort populations by mCherry and GFP...

...target of vaccines and allows the virus to gain entry into human cells via human ACE2 receptors. In their...

...cells through fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that...

...biotin-labeled proteins can them be analyzed by mass spectrometry allowing a researcher to specifically identify...

...showing that this vector is suitable for flow cytometry and fluorescence microscopy, they also show that...

... amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37. doi: 10.1021/bi4000244...

...SARS-CoV-2 would be fully open and available to industry requestors as well. This is a time when we need...

... binding properties. In Journal of Biological Chemistry (Vol. 269, Issue 17, pp. 13048–13055). Elsevier...

... et al. "Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell ...

...it in your experiments. Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke...

...luciferase reporter. CPP was not required for protein entry into cells! After verifying the specificity of their...

...particular interest in monitoring the activity and biochemistry of living neurons in an effort to understand...

...Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat...