We narrowed to 535 results for: try;

1. Tips for Using FRET in Your Experiments

   Type

   Blog Post

   ...brightness of a fluorophore pair, donor:acceptor stoichiometry, and cross-talk between the two fluorophore ...has five-fold less brightness than YFP. The stoichiometry of donor:acceptor is difficult to address especially...

2. Five Popular Model Organisms, Part 2

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   ...popular bacterial organism in microbiology and biochemistry. Certain strains have been specifically developed...studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances in axolotl...

3. The Fluorescent Vegetables in Aptamer Soup

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   ...and U6) and measuring Corn fluorescence by flow cytometry, the Jaffrey group was able to quantify the effect...fluorescence with light-up aptamers." Frontiers in chemistry 4 (2016): 29. PubMed PMID: 27446908. PubMed Central...

4. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J. E., & Gomes, A. V., “Stain-Free...control to β-actin for Western blots. Analytical Biochemistry, 440(2), 186–188. https://doi.org/10.1016/j....

5. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ...applications like fluorescent labeling via click chemistry.    Figure 4: SuperPLD hydrolyzes membrane PC... a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7. https://doi.org/10.1038/s41557-023-...

6. Viral Vectors 101: AAV Variables That Matter

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   ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov &...Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. Journal of Virology...

7. Optogenetics + CRISPR, Using Light to Control Genome Editing

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   Blog Post

   ..., label chromatin, and study kinetics. Using chemistry to photocage CRISPRs The aforementioned techniques...-based Photoactivatable Transcription System. Chemistry & Biology 22:169–174 . https://doi.org/10.1016...

8. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

   Type

   Protocol

   ...detect specific proteins Watch the Video! Immunocytochemistry Use antibodies to detect antigens in cells...

9. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ... Transfection for Recombinant Antibodies Immunocytochemistry Protocol Western Blot Protocol Introduction...

10. Introducing the Addgene Antibodies 101 eBook!

    Type

    Blog Post

    .... If you’re looking to design your first flow cytometry experiment, “Chapter 7: Sorting” is there to help...

11. Lucky Thirteen: Guest Contributors of 2023

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    ...McCartyAntibodies 101: Conventional vs. Flow Spectrometry Author: Lila WittTips for Getting a Faculty ...

12. Antibody Data Repositories and Search Engines

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    ...Differentiation Antigen Workshops Immune cells Flow cytometry (potentially others) Data repository From ...

13. Podcast: A Malawian Professor's Path to Biotech Research

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    Blog Post

    ...one of the best of the best students in his home country. He was selected as one of 600 scholars among 100,000...

14. Addgene Stands with the Scientific Community

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    ... seeking and expanding our collaborations with industry and public and private partners to create additional...

15. How Exercise Purges Stress and Keeps Depression at Bay

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    Blog Post

    ...clue: differences in the kynurenine pathway of tryptophan degradation. It turns out that activation of ...

16. Fluorescent Proteins 101: History of Fluorescent Proteins

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    ...was recognized in 2008 when the Nobel Prize in chemistry was awarded to Shimomura, Chalfie, and Tsien for...

17. Addgene's Top Blog Posts from 2020

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    ...reagents available to scientists in both academia and industry. This year, we also launched the cloning grade...

18. Editor's Choice, September 2016

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    ...Individual Plasmid Formats Better Dyeing Through Chemistry & Small Molecule Fluorophores Addgene's Cancer...

19. Editor's Choice, August 2016

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    ...for another part of my job, I travel around the country and set up meetings with PIs to discuss the benefits...

20. Working 9-5: an Addgene Co-op

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    ... work experience I had was in the food service industry, which followed a very different schedule. Fortunately...

21. Addgene is Expanding Our Viral Vector Service!

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    ...USA); 3–4 days (international; varies widely by country and/or distributor.)  You should plan to receive...

22. Future of Research Conference - Remarkable Opening Session

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    ...Novartis, opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments ...

23. Fostering communication in remote collaborations

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    ...often mean that scientists who live across the country or around the world from each other are collaborating...

24. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

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    ... Society, or maybe you think about a local food pantry or community arts organization. Many people are...

25. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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    ...occasional TikTok. I was also responsible for typical entry level tasks such as stuffing new depositor welcome...

26. Cellosaurus: A Cell Line Information Database

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    Blog Post

    ...names and synonyms will all get you to the right entry.) You can look up the cell lines you are currently...

27. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    ...term though, she did a one year postdoc in the Biochemistry Department at Iowa, but kept up a freelance ...

28. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    ...an application support/sales representative in industry or a postdoc in academia, but knew that, without...

29. 10 Ways to Share Your Science!

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    ...finding a job. Many people in science will work in industry or take on collaborative roles in academia, and...

30. Plasmids 101: Origin of Replication

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    ... amino acid mutagenesis in Escherichia coli. Biochemistry. 2013 Mar 12;52(10):1828-37. doi: 10.1021/bi4000244...

31. Safe Port in a Storm...How Addgene is Weathering the Pandemic

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    ...SARS-CoV-2 would be fully open and available to industry requestors as well. This is a time when we need...

32. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...showing that this vector is suitable for flow cytometry and fluorescence microscopy, they also show that...

33. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

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    ...cells through fluorescence microscopy or flow cytometry. Using the ACE system, the Harris lab showed that...

34. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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    ...biotin-labeled proteins can them be analyzed by mass spectrometry allowing a researcher to specifically identify...

35. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

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    ...organizations such as the Center for Science and Industry (COSI) and the Franklin Park Conservatory. Do ...

36. In Vivo Biotinylation of Bacterial Fusion Proteins

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    ...resonance (Biacore, GE Life Sciences) or bio-layer interferometry (Octet, Pall ForteBio). Tips for using pMal-T-Avi-His...

37. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ... et al. "Super-resolution mapping of neuronal circuitry with an index-optimized clearing agent." Cell ...

38. Hot Plasmids and Viral Preps - September 2021

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    ...transfected with three different vectors and flow cytometry is used to sort populations by mCherry and GFP...

39. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    ...target of vaccines and allows the virus to gain entry into human cells via human ACE2 receptors. In their...

40. Antibodies 101: Chimeric Antibodies

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    ... binding properties. In Journal of Biological Chemistry (Vol. 269, Issue 17, pp. 13048–13055). Elsevier...

41. 10 Great Guest Posts We're Thankful For

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    ...it in your experiments. Better Dyeing Through Chemistry & Small Molecule Fluorophores In this post, Luke...

42. Another Pathway into Cells: iTOP

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    ...luciferase reporter. CPP was not required for protein entry into cells! After verifying the specificity of their...

43. Hot Plasmids: FRET-Based Biosensors

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    ...particular interest in monitoring the activity and biochemistry of living neurons in an effort to understand...

44. Reagent Repositories Are Speeding up Science During the Pandemic

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    ...Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat...

45. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

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    ...variants to eGFP followed by an internal ribosomal entry site and mCherry. If a variant has wild-type levels...

46. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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    ...utilizes her firsthand experience in the software industry to inform her work on emerging issues in novel...

47. Summer SciComm Series: A PhD in Science Communication

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    ...in human anatomy, but I also took classes in biochemistry, microbiology, epidemiology, and bioanthropology...

48. Plasmids 101: Gram Negative and Positive Bacteria

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    ... to help visualize the bleached bacteria. The chemistry behind Gram’s crystal violet stain and the ion...

49. Pushing the Limits of DNA Assembly

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    ... led to advances in biofuel generation, green chemistry and understanding the minimal genome. Many of ...

50. Addgene Depositors Get More Citations

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    ...Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, and Biochemical Research...