...m a father of three, a scientist and science communicator, and I strive to be a volunteer and contribute...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...great feedback from teachers and other science communicators.  What can CRISPR do? I spent more time on...

... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds simple, and it.... et al. (2015) ‘The genome of cultivated sweet potato contains Agrobacterium T-DNAs with expressed genes...

...is the Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations...through barcoded tubes that are scanned into our Laboratory Information Management System (LIMS), a database...operations, she also works on improving laboratory efficiency through automation.       More Resource Avaliable...

...amount of grant funds available and the increasing regulatory process for grants has fostered the demand for...professional. Dr. Cook served as a principal investigator for several research grants. One of the benefits...benefits of being a principal investigator is that you are asked to review grant proposals from others. ...worked for a health department and then for the regulatory services department as our healthy homes program...

...time-consuming, and complex to use outside of standard laboratories. In the case of the COVID-19 pandemic, qPCR ...platforms to reliably detect nucleic acids at the atomolar level. The Zhang lab has adapted natural RNase...reporter enables SHERLOCK to detect DNA or RNA with atomolar sensitivity and single base pair mismatch specificity... of HPV, HPV16 and HPV18, from human cells, at atomolar levels, within one hour. DETECTR thus has the ...

...recently was invited to attend the first Principal Investigator meeting of the NSF EDGE (Enabling Discovery ...parenting strategies and make important ecological indicators since species live in water and on land. Current...that tastes yucky (and is poisonous) for bird predators - birds eat a caterpillar once, vomit, and never... expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants. Arthropods...

...simple as a piece of paper on the front of the incubator containing the passage number, cell line, and ...book and on the actual flasks of cells in the incubator). This means any other scientist who wishes to...The LGC Group also provides standards and has laboratories to carry our independent quality control for...culture contamination." Archives of pathology & laboratory medicine 133.9 (2009): 1463-1467. PubMed PMID...

... vector preps expressing the Voltron2 voltage indicator, created by the Schreiter Lab and the GENIE Project... available! Building on their earlier Voltron indicators (a fusion of Ace2N and HaloTag, Figure 4), they...The same A-to-D mutation in the related voltage indicator Ace2N-mNeon also led to similar improvements in...fluorescent signal at baseline voltage), the investigators found that administering these AAVs with a dilute...

...ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs....Plasmids The ARF (ADP-ribosylation factor) family of regulatory GTPases have long been characterized as members...26893300 ). Though best known for their roles as regulators of membrane traffic, they also play critical ...

...characteristics that make them ideal for studying in a laboratory environment. We’ve covered five model organisms...have been specifically developed to thrive in laboratory settings. One of its most attractive features...characterized genome, and is easily maintained in a laboratory environment. What sets Arabidopsis apart from.... The Norway rat, from an obnoxious pest to a laboratory pet. ELife, 9. https://doi.org/10.7554/elife.50651...

... 37°C heat block, LED blue light illuminator or UV light illuminator. Approach: Highly similar to the ...method based on CRISPR-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch...SARS-CoV-2 Genetic Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang...

...systems such as Arabidopsis, wheat, maize, rice, tomato, and a variety of other species. In addition to... genomes using genome engineering; validated in tomato, tobacco, M. truncatula , wheat, and barley - Daniel...metabolic engineering in plants (including tobacco and potato) - Scott Lenaghan. Joint Modular Cloning (JMC) ...

... serotype displays enhanced transduction of hematopoietic stem cells and microglia. The AAV6(dbY-F+T-V...High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies...

...enChIP-MS - A putative role of HDX as a negative regulator. Hamidian A, Vaapil M, von Stedingk K, Fujita ...ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5.../srep03171. PubMed . Direct identification of insulator components by insertional chromatin immunoprecipitation...

... 58112 tdTomato-MAPTau-C-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58113 tdTomato-MAPTau-...TARDBP GFP CMV ALS Zuoshang Xu 28205 wtTDP43tdTOMATOHA TARDBP HA, tdTomato CAG ALS Zuoshang Xu 28206 TDP43 ...ataxia Richard Possemato 102971 pLKO.1P shFXN_2 FXN Friedreich ataxia Richard Possemato 102975 pLKO.1P...pSLIK-NFLAG-hFUS FUS Flag, His TRE ALS Zissimos Mourelatos 51146 DsRed-p150 217-548 DCTN1 CMV ALS Trina ...tdTomato-MAPTau-N-10 MAPT tdTomato CMV Parkinson's, FTD Michael Davidson 58259 pBabe-Neuroserpin SERPINI1 Dementia Joan....1P shSOD1 SOD1 ALS Richard Possemato 103913 HTRA2_PDZ_1 HTRA2 GST tac Parkinson's Sachdev Sidhu 104448...James Trimmer 182922 hSOD1-FLAG SOD1 CMV ALS Philip Eaton 183984 huntingtin:TOC031-A07:C246751 HTT Flag CMV...

...transcriptional repressor), and dCas9-VP64 (transcriptional activator)-containing plasmids. Each destination vector ...one. This approach is especially useful for combinatorial analyses. For example, if you were to develop...A versatile modular vector system for rapid combinatorial mammalian genetics.” Journal of Clinical Investigation...Cress, Brady F., et al. “CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR Arrays for dCas9...

...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9...multiple serotypes pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes...

...ways scientists have adapted them for use in the laboratory. The discovery of GFP The story of how fluorescent...sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous organisms...studying it grew. In particular, studies in the laboratory of UCSD biochemist Roger Tsien resulted in much...

...research as an NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of ...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...

...Vampire bacteria Vampirovibrio chlorellavorus is a predatory bacteria that attaches to the cell wall of green...forcing it out of hiding to become exposed to predators. Hungry birds spots the “caterpillar” and then... "Back from the dead; the curious tale of the predatory cyanobacterium Vampirovibrio chlorellavorus." ...