We narrowed to 204 results for: Arch-GFP

...Use the search bar or sort buttons to find antibody plasmids for: Common antigens such as GFP or mCherry...intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a...name a few. Traditional sources of antibodies for research purposes include animals or hybridoma cells, but...antibodies extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their...Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description...

.../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* James M. Wilson 105558...9, rh10 James M. Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632...tdTomato 9 William Pu 203842 pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE EF1a EGFP 8 Ivo Spiegel 105550 pAAV.GFAP.Cre.WPRE.hGH...Syn EBFP 5 Hongkui Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB James...pAAV.TBG.PI.FLPo.rBG TBG none 8 Melina Fan , Addgene Research Program Light-Inducible Recombinases ID Name Promoter...

...Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized...synthetic biology collections) for use in C. elegan research. Plasmid...been developed and characterized which allow researchers to express desired constructs in precise cell...highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...computer scientists dedicated to providing the research community with accurate, current, accessible information...

...protein, permeabilized cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48... novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure the ribosome into inserting a fluorophore-tagged...beyond the FRET range of the donor (i.e. >100 Å). Researchers have developed an extensive array of chemical...currently an Assistant Professor in the Anesthesia Research department at Brigham and Women’s Hospital in ... of proteins in living cells." Cell and tissue research 360.1 (2015): 179-194. PubMed PMID: 25743694. ...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...

...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...also includes clone names, which can allow you to search different providers for the same clone. This can...Antibodies community works to make best practices in research antibody selection and use possible, easy, and...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi....

...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...a strategy suited to their research. Find Plasmids for Stem Cell Research Delivery Method Mechanism...layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies focused ...at least one method that can help you with your research goals. Are you a new or experienced reprogrammer...The promise of induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295... Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate...

...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...treatments into cells, and has been widely used in the research community for decades. Wildtype gamma-retroviruses...vectors have many aspects that make them useful research tools in a variety of cell types and models, such...integration, gamma-retroviral vectors are useful research and clinical tools thanks to their long-term expression...practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide for more...Retroviral Vectors and Their Uses in Scientific Research Gamma-retroviral Packaging Systems The Retroviral...

...Edward Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893...115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter...Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Edward...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Eric Schreiter Calcium...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Eric Schreiter Dopamine...Tommaso Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Tommaso Patriarchi Oxytocin...nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors iATPSnFR2 5-HT...

...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team demonstrated...undoubtedly constitute a valuable tool for the research community. You can find the AAV vectors for infecting...imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin and Ryohei Yasuda ...mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring...

...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and...system of choice, promoter, or search by inducible system (e.g., search for "split", "rapamycin", “tamoxifen...been limited by low integration efficiency but research groups continue to optimize these tools. The best...recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort buttons to find plasmids based on ...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Flp recombinase...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Dre recombinase... gene expression may be activated or repressed. Search by recombinase (Cre, Flp, Dre, etc.) or target ...

...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...treatments into cells, and has been widely used in the research community for decades. Wild-type lentiviruses ...vectors have many aspects that make them useful research tools in a variety of cell types and models. Lentiviral... of a transgene, lentiviral vectors are useful research and clinical tools. Stable Cell Lines Lentiviral...best practices with regard to lentiviral-related research. See Addgene’s Biosafety Resource Guide for more...accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration...

...peak action spectra Example: Jaws Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is ...589 Archaerhodopsins Arch Light-activated outward proton pump from Halorubrum sodomense 566 eArch 3.0 ...TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from...3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated outward proton pump from...used to inhibit neurons in optogenetic experiments. Arch is a light-activated outward proton pump that hyperpolarizes...) the cell when triggered by green-yellow light. Arch variants have been developed with the following ...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...

...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...MoClo Plasmid Kits Available from Addgene Browse or search the table below to find MoClo kits in our collection...Johannes Stuttmann 95 additional plasmids for hierarchical assembly of plant multigene constructs. MoClo...characterized parts that can be used for bottom-up hierarchical assembly of single and multi-gene constructs...

...vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB...viral vectors repository this year (and it's only March!)  In our repository, you'll find the latest iteration...Chemogenetics Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral ...

...plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity of the genetic...codon-amino acid pairs. Over the past two decades, researchers have also expanded the genetic code by redirecting...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies; MilliporeSigma F- mcrA Δ(mrr-hsdRMS-mcrBC...information to aid in the reproducibility of scientific research. Long-read Sequencing Sanger sequencing and Illumina...

...fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression cassette...halves of EGFP to recombine by homology directed repair, and resulting in the expression of EGFP. Using ...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...strand annealing) took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid...pCAG-EGxxFP target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The...can be placed in multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized ...

...fluorescent tool with magnets would enable new research and applications that don’t yet exist, but it ... this was even possible. There is a variety of research on whether and how organisms respond to magnetic... non-biological conditions. So, they decided to search for a magnetic response among other proteins that...protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable change in fluorescence...better, multiple other labs have reproduced their EGFP findings since the initial report in 2023.)   ...   Figure 1: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 mT...proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made a reliable one-component...

...Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP 50457 AAV2 pAAV-hSyn-DIO-EGFP   Biosensor AAV...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype...H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns... Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO mCherry ...sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters... viral protocols and videos Find AAVs for your research ...

... acceptor GFP2-Gγ chimeras, and two untagged Gβ constructs. For the Gγ constructs, the GFP2 tag is fused... Gα-Rluc8 and Gγ-GFP2 will be in close association, resulting in a high level of GFP2 emission. Following... TRUPATH work? The TRUPATH collection allows researchers to measure the physical association of combinations...interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2 and Gβ constructs. Cells are then treated with the...complex will disassociate, resulting in a decreased GFP2 emission. The activation state can be measured by... by a BRET2 ratio, which is the ratio of GFP2 emission to RLuc8 emission (see figure). What can TRUPATH...