We narrowed to 205 results for: Arch-GFP

...and labeled with primary antibody (0.01 µg/mL Anti-GFP [N86/38.1R] (Addgene #180084), 0.01 µg/mL Anti-HA...Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079) to create pAG Lenti CMV HA-EGFP Puro (Addgene...developed by the GENIE Project team at HHMI's Janelia Research Campus (Aggarwal et al., 2025).In this latest ...genetic cargo of choice. After hearing about researchers' favorite features and limitations of existing...excited to share our very own set of empty, HA-, and EGFP-expressing lentiviral backbones designed right here... packaging and strong expression after inserting EGFP and provide step-by-step protocols for viral generation...Figure 2). Looking for more? Explore our empty and EGFP retroviral plasmid set, our bacterial plasmid set...

...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...treatments into cells, and has been widely used in the research community for decades. Wildtype gamma-retroviruses...vectors have many aspects that make them useful research tools in a variety of cell types and models, such...integration, gamma-retroviral vectors are useful research and clinical tools thanks to their long-term expression...practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide for more...Retroviral Vectors and Their Uses in Scientific Research Gamma-retroviral Packaging Systems The Retroviral...

.../Tag Serotype(s) PI 105551 pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 CamKII GFP 9, rg* James M. Wilson 105558...9, rh10 James M. Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632...Syn EBFP 5 Hongkui Zeng 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn eGFP 1, 2, 5, 8, 9, rg*, PHPeB James...pAAV.TBG.PI.FLPo.rBG TBG none 8 Melina Fan , Addgene Research Program Light-Inducible Recombinases ID Name Promoter...

...Lab. A set of vectors for C. elegans research, including lacZ and GFP fusion vectors. C. elegans optimized...synthetic biology collections) for use in C. elegan research. Plasmid...been developed and characterized which allow researchers to express desired constructs in precise cell...highlights plasmids that contain tools for use in worm research. This includes CRISPR, RNAi, empty backbones, ...complete list of plasmids that can be used in worms, search Addgene's entire collection. ID Plasmid Description...computer scientists dedicated to providing the research community with accurate, current, accessible information...

...Use the search bar or sort buttons to find antibody plasmids for: Common antigens such as GFP or mCherry...intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a...name a few. Traditional sources of antibodies for research purposes include animals or hybridoma cells, but...extensively validated for neuroscience and other research applications: NeuroMab/Trimmer Lab Recombinant...peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies and their...Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description...

...protein, permeabilized cells  Cy5NTAc 32 25-45 GFP (fluorescein is similar)   Lumio Red Tc: CCPGCC 48... novel aminoacyl tRNA synthetase/tRNA pair, a researcher can lure the ribosome into inserting a fluorophore-tagged...beyond the FRET range of the donor (i.e. >100 Å). Researchers have developed an extensive array of chemical...currently an Assistant Professor in the Anesthesia Research department at Brigham and Women’s Hospital in ... of proteins in living cells." Cell and tissue research 360.1 (2015): 179-194. PubMed PMID: 25743694. ...

...labeling. Size is also a factor. Bulkier tags, such as GFP, may interfere with protein expression or function...processes that are crucial in the world of biological research, medical therapy, and even drug development (Grimm...stable, and versatile imaging tools in biological research. This does make finding the right dye for your...complicated. We hope this post helps you in your search for the perfect way to light up your science. Simon...

...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...treatments into cells, and has been widely used in the research community for decades. Wild-type lentiviruses ...vectors have many aspects that make them useful research tools in a variety of cell types and models. Lentiviral... of a transgene, lentiviral vectors are useful research and clinical tools. Stable Cell Lines Lentiviral...best practices with regard to lentiviral-related research. See Addgene’s Biosafety Resource Guide for more...accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration...

...measurable through an epitope tag such as FLAG/His/GFP. Fluorescent protein tags, which correlate closely...and flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, ...also includes clone names, which can allow you to search different providers for the same clone. This can...Antibodies community works to make best practices in research antibody selection and use possible, easy, and...quantification of antibody performance in major research applications. eLife, 12, RP91645. https://doi....

...cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from cells by dilution...a strategy suited to their research. Find Plasmids for Stem Cell Research Delivery Method Mechanism...layers. Choosing a reprogramming method Most iPSC research falls into two categories: 1) studies focused ...at least one method that can help you with your research goals. Are you a new or experienced reprogrammer...The promise of induced pluripotent stem cells in research and therapy." Nature 481, no. 7381 (2012): 295... Cells Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate...

...Edward Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893...115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter...Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Edward...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Eric Schreiter Calcium...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Eric Schreiter Dopamine...Tommaso Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Tommaso Patriarchi Oxytocin...nLight Oxytocin (OT) Sensors GRAB_OT Voltage Sensors Archon Voltron JEDI-2P ASAP ATP Sensors iATPSnFR2 5-HT...

...DOG New Tricks: Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and...system of choice, promoter, or search by inducible system (e.g., search for "split", "rapamycin", “tamoxifen...been limited by low integration efficiency but research groups continue to optimize these tools. The best...recombinase (Cre, Flp, Dre, other recombinases). Use the search bars or sort buttons to find plasmids based on ...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Flp recombinase...Vector Type Promoter PI Additional Addgene resources Search our full catalog for plasmids expressing Dre recombinase... gene expression may be activated or repressed. Search by recombinase (Cre, Flp, Dre, etc.) or target ...

...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team demonstrated...undoubtedly constitute a valuable tool for the research community. You can find the AAV vectors for infecting...imaging of two biosensors simultaneously   Researchers in the labs of Michael Lin and Ryohei Yasuda ...mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring...

...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...MoClo Plasmid Kits Available from Addgene Browse or search the table below to find MoClo kits in our collection...Johannes Stuttmann 95 additional plasmids for hierarchical assembly of plant multigene constructs. MoClo...characterized parts that can be used for bottom-up hierarchical assembly of single and multi-gene constructs...

...plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity of the genetic...codon-amino acid pairs. Over the past two decades, researchers have also expanded the genetic code by redirecting...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies; MilliporeSigma F- mcrA Δ(mrr-hsdRMS-mcrBC...information to aid in the reproducibility of scientific research. Long-read Sequencing Sanger sequencing and Illumina...

...vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype pAAV-CaMKIIa-EGFP AAV PHP.eB...viral vectors repository this year (and it's only March!)  In our repository, you'll find the latest iteration...Chemogenetics Deisseroth New viral vector pAAV.CMV.PI.EGFP.WPRE.bGH  AAV PHP.eB Controls Wilson New viral ...

...identify materials that may be useful to your research. But searching our website also allows for filtering for... empty backbones such as mEGFP-N1 for expression in mammalian cells or mEGFP-pBAD for bacterial expression...As Addgene’s collection of research tools available to industry researchers continues to expand, we encourage... barriers, facilitating access to high-quality research materials, tools, and resources, while also fostering.... While Addgene is well known for facilitating research at nonprofit organizations, we have always sought...sought to support scientists at all research institutions. In 2012, we shipped our first plasmid to an ...we wanted to highlight a few of these exciting research tools. The materials available to industry cover...

...fluorescent protein (EGFP) reconstitution. (a) Scheme of validation for DSB mediated EGFP expression cassette...halves of EGFP to recombine by homology directed repair, and resulting in the expression of EGFP. Using ...adaptation for use in mammalian cells in 2012-2013. Researchers are now using this system in ever more creative...Hopefully this post will help get your in vivo research up and running as soon as possible! Selecting ...strand annealing) took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid...pCAG-EGxxFP target plasmid contains overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The...can be placed in multi-cloning site (MCS) between EGFP fragments. The pX330 plasmid contains humanized ...

...fluorescent tool with magnets would enable new research and applications that don’t yet exist, but it ... this was even possible. There is a variety of research on whether and how organisms respond to magnetic... non-biological conditions. So, they decided to search for a magnetic response among other proteins that...protein they tested — E. coli expressing good ol’ EGFP — showed a slight but detectable change in fluorescence...better, multiple other labs have reproduced their EGFP findings since the initial report in 2023.)   ...   Figure 1: Fluorescence intensity of EGFP-FlavinTag in E. coli changes in response to a ~25 ...proteins display a similar effect (many did!). Fusing EGFP to the self-labeling FlavinTag made a reliable one-component...

...Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP 50457 AAV2 pAAV-hSyn-DIO-EGFP   Biosensor AAV...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype...H134R)-EYFP   Control AAV Control AAV allow researchers to not only interpret their results, but also...to achieve different patterns of expression, researchers often need to determine these transduction patterns... Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE 114471 AAV1, AAV5 pAAV-Ef1a-fDIO mCherry ...sensors: Dopamine and norepinephrine Neuroscience research relies on accurate monitoring of neurotransmitters... viral protocols and videos Find AAVs for your research ...