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We narrowed to 314 results for: Cas9

Showing: 161 - 180 of 314 results

  1. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...catalytically inactive (dead), human codon-optimized Cas9 under the control of Murine Stem Cell retrovirus...backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used to produce a... (shRNA, miR-30-shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together with expression of ...and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression of a catalytically...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...using CRISPR/Cas9 to edit a gene, the expression of Cas9 is no longer necessary. However, Cas9 can be toxic...like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing inducible...

  4. Zebrafish Plasmid Collection

    Type
    Collection
    ...Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector System...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart...resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful...GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease...use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally validated...

  5. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759...none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...your gene of interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit.... "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans." Genetics...blog Learn more about SapTrap Read about CRISPR/Cas9 in C. elegans Browse our Plasmids 101 blog posts...

  7. CRISPR Plasmids - Yeast

    Type
    Collection
    ...) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49014... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding...handle_RPR1t pRPR1 HindIII S. cerevisiae LEU2 none, need Cas9 plasmid Lu Do you have suggestions for other plasmids...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...Marker PI Publication Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a ...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Bentley For RNA-guided genome editing, think beyond Cas9 with STITCHR: a combined CRISPR / retrotransposon... Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein, ...for scarless insertion of the payload (teal). The Cas9 gRNA targeting guide and the transposon RNA homology...scientists fused the retrotransposon protein R2Tocc to a Cas9 nickase to improve target recruitment and nicking...TurboCas provides the genomic targeting power of dCas9 with the dynamic labeling power of miniTurboID, ...proximity ligation tool (Cenik et al., 2024). While dCas9 has been used in combination with proximity labeling...background labeling. By fusing miniTurboID with dCas9 to create TurboCas, the Shilatifard Lab created ...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...the development of site-specific nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges ...piggyBac™ transposon system can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in ...along with a site-specific nuclease (such as CRISPR/Cas9) to create a double-stranded DNA break. After DNA...technologies including Zinc Fingers, TALENs, and CRISPR-Cas9 nucleases. Mathew applies his specialized knowledge...

  10. CRISPR Plasmids - Tagging

    Type
    Collection
    ...pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged Cas9 AND a gRNA... consists of two components: A vector containing Cas9 and a validated gRNA, based on the Zhang lab's PX458...need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design...consists of two components: An all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously... to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for selectable genome...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  11. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...and CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs...Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality Control for Pooled... CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9 Day 10: Transcriptional regulation:...The PAM Requirement and Expanding CRISPR Beyond SpCas9 Day 8: Repair mechanisms: CRISPR 101: Non-homologous...

  12. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method..., relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish ..., and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these...

  13. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...PASTEsystem involves insertion of landing sites via Cas9-directed reverse transcriptases, followed bylanding...bylanding site recognition and integration of cargo via Cas9-directed integrases.   Use PASTE can be delivered...adenovirus templates. The system, which is larger than Cas9, can also be used with mRNA and a synthetic guide...

  14. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... third of the size of Cas9). Figure 2: Comparison of TnpB (A) and Cas9 (B) genome editors. Effector..., recruited by either the ωRNA (TnpB) or a gRNA (Cas9). Drawings not to scale. Created with BioRender....

  15. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ... the genome. They fused a catalytically impaired Cas9 protein to a DNA-editing enzyme to generate the ...based on a prime editor which is a fusion between Cas9 and reverse transcriptase. Using an elongated template...The new frontier of genome engineering with CRISPR-Cas9. Science 346:1258096 . https://doi.org/10.1126/science...Zhang F (2013) Genome engineering using the CRISPR-Cas9 system. Nature Protocols 8:2281–2308 . https://doi.org...

  16. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ...Tools CRISPR Blog Posts A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes... CRISPR dCas9-FokI CRISPR: dCas9-FokI...with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...gRNAs, binding ~15-25 bp apart, are required for dCas9-FokI to dimerize at a given region of the genome...

  17. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help...Promoter Selectable Marker Publication Prime Edit Cas9 H840A nickase fused to a reverse transcriptase (...PI Publication Interfere Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors... CRISPR Blog Posts Base Edit Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a ...

  18. RUBY-Red Siliques

    Type
    Blog Post
    ...transgene free individual. For example, with CRISPR-Cas9 gene editing, transformation may be identified by...CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 Tips for Arabidopsis Transformation CRISPR 101: ...

  19. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several...fusion for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids... plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion...175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798...

  20. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi...Anzalone et al., 2019, including modifications to the Cas9 or reverse transcriptase enzymes, inhibition of ...end of the pegRNA, which is not well protected by Cas9. This is similar to the proposed mechanism for engineered...of applications, including CRISPR activation by dCas9-VPR, prime editing, and delivery of disease-relevant...
Showing: 161 - 180 of 314 results