Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 636 results for: ESP

Showing: 161 - 180 of 636 results

  1. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...about the people that don’t respond; focus on the nice people who do respond. Don’t call or email multiple...multiple times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ... council – all worked in tandem, within their respective mandates, to tackle a controversial and ethically...” or whether the media and academic conference response to He created a positive feedback loop that rewarded..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...

  3. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...research. These fluorescent proteins light up in response to calcium, which is more than just the stuff ...mouse cortex. Bottom: Zoomed-in view of traces corresponding to the dashed box above. The number of action...indicates a single AP. B) Averaged fluorescence responses to increasing numbers of evoked APs in neurons...

  4. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...preparation and a willingness to embrace new responsibilities. After my second postdoc, I realized that... team and allow myself to focus on my core responsibilities, which typically revolved around the broader...started at my previous company by taking on responsibilities such as mentoring a junior colleague. Guiding...

  5. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...correlating them with tumor mutations and clinical responses. This platform has the potential to become an ... to control immune signaling and inflammatory responses remotely with spatiotemporal precision. This is...function of immune cells that are not excitable but responsive to calcium. We’d love to expand our cancer and...

  6. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

    Type
    Blog Post
    ... available collection of mutant cDNA clones corresponding to cancer variants found in genome sequencing...include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived from analysis of ...aggregate a sequence-confirmed series of clones corresponding to known cellular signaling pathways as part...

  7. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...what will be packaged. The 5’ LTR and 3’ LTR, respectively, act as RNA polymerase II promoter and terminator...packaging of the virus into particles. E2A/E2B are responsible for viral replication, with E2B being the primary... suppresses both apoptosis and the host immune response to the virus to allow it to evade detection by...

  8. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...vaccines, including 1) cellular responses in addition to antibody response 2) very high immunogenicity 3... 3) and long-lasting immune response even after a single dose. Vaccine development strategies. Image...

  9. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...stimulates the overall immune response. Once the initial antibody response starts to wane about a month...include any antibodies produced during the immune response.   Polyclonal antibodies are generated ...

  10. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...always a good idea to perform primer optimization, especially if you are having difficulty amplifying your ...colonies, check that the antibiotic in the plate correspond to the antibiotic resistance marker present in...small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract the plasmid, and analyze...

  11. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ... as channelrhodopsins (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing...channelrhodopsin-expressing neurons, the channelrhodopsins respond by opening and allowing an influx of ions into ...expressing mice did not have this self-stimulation response. This result showed that ChRger2 stronger photocurrent...

  12. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...to a large family of solute channels that are responsible for maintaining the nutrient and ionic balance...of an autoinflammatory loop: keratinocytes both respond to and release IL-36 cytokines. Keratinocytes are...cell and translate those signals into cellular responses. For example, GPCRs and their downstream G protein...

  13. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientific publishing has a number of drawbacks, especially the increasingly growing time from submission... a few individuals.  Early-career researchers especially enjoy the ability to post a preprint as evidence...editors’ comments, reviewers’ comments, and author responses can be shared directly alongside the preprint ...

  14. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

  15. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...lox system is a well-established research tool, especially in the field of mouse transgenics. A few of the... (FRT) site being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective ...

  16. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ... using AdV is that it triggers a strong immune response after in vivo administration, which results in...expression ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent...spectrophotometry. An absorbance of at 260nm corresponds to 1.1 x 1012 particles/mL. To calculate the ...

  17. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...lack all viral genes, including those that are responsible for integration into host chromosomes, further... (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated mutations...PMCID: PMC4450362. 4. Sun, J. Y., et al. "Immune responses to adeno-associated virus and its recombinant ...

  18. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ... and AsCpf1 are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9....These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter than those...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

  19. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...communication and learning to work with people despite the distance improved my verbal and written communication...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need... different hats and came to understand the responsibilities that come with leadership. The NPA was a great...

  20. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...with the Krauthammer lab (University of Zurich), especially with my deep learning-wizard colleague Ahmed ...selecting the most promising pegRNA designs and especially facilitates the use of prime editing in pooled...true for any prime editor experimentalist, and especially shines once large-scale prime editing experiments...
Showing: 161 - 180 of 636 results