...Regulating translation is key to cellular function, especially during development or stress. With ribosome profiling...devised a system that labels both mRNAs and their corresponding newly synthesized proteins. As in the TRICK ... usually translated in a specific compartment. Despite these limitations, these techniques represent powerful...

... council – all worked in tandem, within their respective mandates, to tackle a controversial and ethically...” or whether the media and academic conference response to He created a positive feedback loop that rewarded..., and they are not remembered kindly. However, despite many headlines questioning if He’s experiments ...

...about the people that don’t respond; focus on the nice people who do respond. Don’t call or email multiple...multiple times if you don’t get a response. It breaks an important law of networking--don’t be creepy. There...

...correlating them with tumor mutations and clinical responses. This platform has the potential to become an ... to control immune signaling and inflammatory responses remotely with spatiotemporal precision. This is...function of immune cells that are not excitable but responsive to calcium. We’d love to expand our cancer and...

... available collection of mutant cDNA clones corresponding to cancer variants found in genome sequencing...include 634 sequence confirmed mutant cDNA clones corresponding to cancer variants derived from analysis of ...aggregate a sequence-confirmed series of clones corresponding to known cellular signaling pathways as part...

...what will be packaged. The 5’ LTR and 3’ LTR, respectively, act as RNA polymerase II promoter and terminator...packaging of the virus into particles. E2A/E2B are responsible for viral replication, with E2B being the primary... suppresses both apoptosis and the host immune response to the virus to allow it to evade detection by...

...vaccines, including 1) cellular responses in addition to antibody response 2) very high immunogenicity 3... 3) and long-lasting immune response even after a single dose. Vaccine development strategies. Image...

...preparation and a willingness to embrace new responsibilities. After my second postdoc, I realized that... team and allow myself to focus on my core responsibilities, which typically revolved around the broader...started at my previous company by taking on responsibilities such as mentoring a junior colleague. Guiding...

...always a good idea to perform primer optimization, especially if you are having difficulty amplifying your ...colonies, check that the antibiotic in the plate correspond to the antibiotic resistance marker present in...small culture volume (2 or 3 ml) containing the corresponding antibiotic, extract the plasmid, and analyze...

... as channelrhodopsins (ChRs) makes them light-responsive. When light shines on excitatory channelrhodopsin-expressing...channelrhodopsin-expressing neurons, the channelrhodopsins respond by opening and allowing an influx of ions into ...expressing mice did not have this self-stimulation response. This result showed that ChRger2 stronger photocurrent...

...to a large family of solute channels that are responsible for maintaining the nutrient and ionic balance...of an autoinflammatory loop: keratinocytes both respond to and release IL-36 cytokines. Keratinocytes are...cell and translate those signals into cellular responses. For example, GPCRs and their downstream G protein...

...stimulates the overall immune response. Once the initial antibody response starts to wane about a month...include any antibodies produced during the immune response.   Polyclonal antibodies are generated ...

...scientific publishing has a number of drawbacks, especially the increasingly growing time from submission... a few individuals.  Early-career researchers especially enjoy the ability to post a preprint as evidence...editors’ comments, reviewers’ comments, and author responses can be shared directly alongside the preprint ...

...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab...at some point, decide early on what to do in a namespace collision. It may even be worth it to have extra... you're solving, but consistency is key. Some namespace collisions you may encounter: vector, sequence...

...cyclization recombinase”), is a 38 kDa protein responsible for intra- and inter-molecular recombination ...lox system is a well-established research tool, especially in the field of mouse transgenics. A few of the... (FRT) site being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective ...

... using AdV is that it triggers a strong immune response after in vivo administration, which results in...expression ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent...spectrophotometry. An absorbance of at 260nm corresponds to 1.1 x 1012 particles/mL. To calculate the ...

...lack all viral genes, including those that are responsible for integration into host chromosomes, further... (HDR). This characteristic makes AAV vectors especially amenable to correcting disease-associated mutations...PMCID: PMC4450362. 4. Sun, J. Y., et al. "Immune responses to adeno-associated virus and its recombinant ...

... and AsCpf1 are 3.7 kb and 3.9 kb in length, respectively, larger than SaCas9 but smaller than SpCas9....These nucleases should fit well into AAV vectors, especially given that Cpf1 gRNAs are also shorter than those...rate is similar to that of high fidelity Cas9s eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins...

...communication and learning to work with people despite the distance improved my verbal and written communication...strengths and work effectively. Leadership responsibilities Being a leader in an organization, you need... different hats and came to understand the responsibilities that come with leadership. The NPA was a great...

...there’s a PAM near your insertion site, the corresponding gRNA might be undesirable due to potential off-target...than SpCas9, in this case, NNGRRT and NNNNGATT, respectively. Researchers also engineered SpCas9 variants..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...