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We narrowed to 471 results for: FLU;

Showing: 161 - 180 of 471 results

  1. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...you can use PCR based detection, DNA staining, fluorescent tagging, agar plating, etc. Be wise to choose... to detect mycoplasma. When observed under a fluorescent microscope, the mycoplasma DNA appears in the...small clusters, apart from the cellular DNA. Fluorescent DNA staining is another DNA staining alternative...contamination by other bacteria or extracellular fluorescent signals produced by these bacteria can hinder...culture media. Also, drugs like BM Cyclin, fluoroquinolone ciprofloxacin, ciprobay, zagam, baytril, tetracycline...Method for the Detection of Mycoplasmas Using Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390...

  2. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...used for western blot, flow cytometry, and immunofluorescence . However, protein purification is not ideal...amino acid peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively charged...for immunoprecipitation, western blot, and immunofluorescence with some success for protein purification...and can serve as an epitope tag as well as a fluorescent marker. The GFP tag is ideal for live cell imaging...imaging, FRET, flow cytometry, or immunofluorescence where an antibody can’t be used or that step can ...

  3. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...various structured surfaces. Plasmids expressing fluorescent proteins will be used to tag individual strains...within the biofilms will then be monitored using fluorescence microscopy and FACS. In an additional approach...this condition is crucial. Plasmids expressing fluorescent proteins and a new two-photon laser scanning ...plasmids containing promoter regions that activate fluorescent protein transcription in response to river contaminants...water samples at specific locations, measure fluorescent signals from the sensor, and send a signal including...

  4. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...separate DNA or transforming cells with green fluorescent protein, these activities are limited to those...Typically, if you want to make a protein – say green fluorescent protein – you first need the DNA sequence that... instructions it needs to produce that green fluorescent protein through transcription and translation...students can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can ...Stark, Jessica C., et al. "BioBits™ Bright: A fluorescent synthetic biology education kit." Science Advances...

  5. Penn Vector Core Partnership with Addgene

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    Collection
    ...James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917 105541...James M. Wilson AV-8-PV0105 105532-AAV8 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-8-PV0146 105535...Karl Deisseroth AV-5-PV0105 105532-AAV5 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-5-PV1090 105537-AAV5... M. Wilson AV-8-PV1302 105538-AAV8 pENN.AAV.TBG.PI.ffLuciferase.RBG James M. Wilson AV-8-PV3637 65015-...Karl Deisseroth AV-9-PV0105 105532-AAV9 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-9-PV0109 105533-AAV9...Karl Deisseroth AV-2-PV0105 105532-AAV2 pAAV.CMV.ffLuciferase.SV40 James M. Wilson AV-1-PV3365 105554-AAV1...

  6. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...microenvironment enzymes and metabolic reprograming influence tumors development? Being pathological active ...to cure cancer. To understand how cancer cells influence microenvironments and vice versa, let’s take a... being shaped by them. Another set of highly influential microenvironment cells are Cancer-Associated ...fact, Sanford H. Barsky and his team tested the influence myoepithelial cells had on the conversion of non-malignant...begun to understand two main areas of study that influence tumor microenvironments: immunology and angiogenesis...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...implications for the fusion protein effectivity), and a fluorescent protein tag for visualization (Fig. 1b). While...catalytically inactive dCas9) to modify chromatin. A fluorescent protein can still be included for visualization....   G-baToN can also be applied to other fluorophore/antibody pairs. Because the anti-GFP nanobody...quantify protein degradation the Ward lab used fluorescent proteins (FPs) fused to the AID* (minimal 44 ...Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair pathways, Amit Choudhary's lab developed...

  8. Hot Plasmids February 2024

    Type
    Blog Post
    ...colleagues identified a highly bright and stable fluorescent protein, StayGold. Although it quickly saw wide...dimerization while maintaining the bright and stable fluorescence of the original (Ivorra-Molla et al., 2023)....Ivorra-Molla, E., et al. (2023). A monomeric StayGold fluorescent protein. Nature Biotechnology, 1–4. https://doi.org..., et al. (2023). Bright and stable monomeric fluorescent proteins derived from StayGold. ResearchSquare...mature marmosets. CAP-Mac also robustly expressed fluorescent reporters in green monkeys and cultured human...

  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ... FAST system, Ye’s lab could edit a tdTomato fluorescent reporter gene in mice using Minicircle DNA vectors...of Cas9 and a photo-dissociable dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa... flanking the DNA-binding cleft of Cas9, the fluorescent domains dimerize and prevent DNA binding in the...chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins can be used to identify specific...gRNA and a dCas9 fusion protein with three red fluorescent tags in U2OS cells. Upon light activation, dCas9...figure below. Next, by using a dual reporter fluorescence assay, Hemphill et al. demonstrated that the...

  10. Plasmids 101: Protein tags

    Type
    Blog Post
    ...Which Fluorescent Protein Should I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent Protein...Plasmids 101 has covered GFP and its related fluorescent proteins, which are sometimes used as tags for...tag for purification? Or you want to combine a fluorophore with a tag that localizes your protein to the...

  11. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...better ability to record subthreshold voltage fluctuations, while having high fidelity during action potentials...Structure of Voltron or Voltron2 conjugated to a JaneliaFluor dye emitting at 525 nm. Image reused from https...Because they are negative-going sensors (with fluorescent signal at baseline voltage), the investigators...closely-packed labeled cells. Plus, Voltron2’s fluorescence is tunable by changing the dye that becomes ...6).   Figure 6: Illustration of split fluorophore assay to test reconstitution via mRNA trans-splicing...

  12. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...The SunTag system uses an scFv to amplify the fluorescence intensity of a tagged protein. SunTag has two...bind the SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule...contains an HA-binding scFv that’s fused to a fluorescent protein such as GFP. The HA frankenbody works...frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies  Read about...SunTag system which uses an scFv to amplify the fluorescence intensity of a tagged protein. Resources on...

  13. Plasmid Collections

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    Collection
    ...and pooled libraries. Find tools for CRISPR, fluorescent proteins, optogenetics, biosensors, and more....CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent Proteins Luciferase Optogenetics Chemogenetics... set. Click here to view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways ...collections. Depositor Collection Types CRISPR Fluorescent Proteins Protein Expression Synthetic Biology...

  14. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...transporters. The intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by ...primary visual cortex (V1) layer 2/3 (L2/3). B) Fluorescence from axonal boutons of neurons expressing iGluSnFR3... IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Thermo Fisher A-21131) or Goat anti-Rabbit ... (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (Thermo Fisher A-11012), and counterstained ... optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of ...

  15. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...26322837. 2. Stadler, Charlotte, et al. "Immunofluorescence and fluorescent-protein tagging show high correlation...displayed good binding efficiency in both immunofluorescence and immunoprecipitation applications, but...Plasmids Tag endogenous proteins with CRISPR Fluorescently Tag Your Protein of Interest ...

  16. Worm Expression Resources

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    Collection
    ... which makes them attractive for imaging and fluorescence microscopy experiments. The C. elegans genome...This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large ...and GFP fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without...Approach to Better CRISPR/Cas9 Editing Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even...

  17. Allen Institute for Cell Science Plasmid Collection

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    Collection
    ... Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in... Like... CRISPR Guide CRISPR Protein Tagging Fluorescent Protein Guide Subcellular Localization Plasmids... collection (Link opens in a new window) of fluorescently tagged, human, induced pluripotent stem cell...plasmids researchers will be able to generate fluorescently tagged proteins of key cellular structures in...

  18. Lentiviral Prep Service

    Type
    Collection
    ...expresses EGFP for easy visualization via direct fluorescence. Morris 115644 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris 115645 Morris CellTag Barcode Library...expresses EGFP for easy visualization via direct fluorescence. Morris Control Lentivirus Browse our list of...based on plasmids in our repository that express fluorescent proteins. ID Name Insert Antibiotic Resistance...

  19. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...fused to a fluorescent protein (FP) can help visualize specific genomic loci using fluorescent microscopy...repetitive regions. Fluorophores may also be fused to gRNA sequences to produce fluorescence at a target region...

  20. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

    Type
    Blog Post
    ...element (tyrosine sulfation consensus sequence, fluorophore, or APEX2), allowing for quantitative analysis... Since the anti-GFP nanobody is able to bind fluorescent proteins derived from A. victoria the functionalized...fused with GFP, YFP, mCerulean, or other related fluorophores. Find all of the nanobody toolkit plasmids here...technologies to quantify proteins in tissues, cells, and biofluids. Recently, Sam Myers, a Scientist in the group...
Showing: 161 - 180 of 471 results