We narrowed to 609 results for: GAN
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TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR...
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Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post... on the advisory board for Art in Giving, an organization that raises money for childhood cancer through...art. About two years ago, I had a big role in organizing an Art in Giving think tank event where we brought...proposal, which Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful...wonderful! I also volunteer for a dog rescue organization where I foster dogs and help screen dog adoption... -
Why Do I Need an MTA Anyway?
TypeBlog Post... for more details. Benefits to the provider (organization / lab that owns the material) Affirmation ...materials will only be shared with non-profit organizations Assurance that recipient may not use the ...Limited liability Benefits to the Recipient (organization / lab receiving the material) Permission ...directly to your institution’s MTA coordinator. Organizations that have opted into our eMTA system can even... -
Let There Be LITE Plasmids
TypeBlog Post...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... making use of the new optogenetic tools in C. elegans and in Plasmodium. For those who do want to apply... Kendall Morgan... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...research is vital in combating misinformation and propaganda that limits access to full reproductive health...-based policy and legislation hit home hard in Michigan, where programs like the Great Lakes Restoration...planet. And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use...a postdoctoral researcher in the University of Michigan Department of Astronomy. She studies how planets...I was also incredibly impressed with how well organized the march was, as well as the friendly atmosphere... -
Science as Art
TypeBlog Post... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog Post... the organization of the nucleus in space and time, and to understanding how nuclear organization varies...varies across the life of a cell, or how organization may be altered in disease states. dCas9 gets a colorful...work to increase our understanding of chromatin organization. This fluorescent CRISPR/Cas9-based method is... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from. Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed... -
AAV Packaged on Request
TypeCollection...Approval 1+ business days, varies by requesting organization Viral vector preps remain subject to the terms... -
Safe Port in a Storm...How Addgene is Weathering the Pandemic
TypeBlog Post...succeed at the same time. I began approaching science funding organizations to request support for our ..., cures, and diagnostics. Almost immediately we began to receive urgent requests for access to coronavirus-related...were facing a financial crisis. As a nonprofit organization, Addgene charges enough for our services to ... -
Meet the New Addgene Board Members!
TypeBlog Post...,” Baradaran says. Baradaran’s path to science began in high school. “I had a great chemistry teacher...this is what I wanted to do,” Stegmeier says He began this journey in biochemistry made the foray into... at Addgene. While deposits from for profit organizations are less common, they can be very impactful.... diseases and over the past few months, the lab began a project to develop an AAV-based COVID-19 vaccine... -
Viral Vectors 101: Transductions
TypeBlog Post...depends on two things. First, what type of cell or organism context you are targeting? Second, what is the...moderate levels. With regard to target cells or organism, some primary cells and differentiated cell types...shine is when a specific cell type within a live organism is the target. Although rarely done, viral pseudotype...for a type or subtype of cells. This allows an organism to be injected with a virus, but enables tissue-specific... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog Post...tool to advance our knowledge of brain-visceral organ signaling. The problem Previously, there were... for monitoring how both the brain and visceral organs interact during various behaviors. Either tissue...common neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar...applications are to expand the tool to different brain-organ axes. Alternatively, the system could be used to... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...Everyone workshop at TAGC with the three other organizers. We asked them about their experiences as they...Reproducibility for Everyone Workshop, Abitua and the other organizers found several avenues to keep the audience engaged...conference was over. Depending on the conference, organizers may make the poster available after the conference...actively reach out. It’s more proactive versus organic networking,” she says.Depending on the meeting,... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...also another free citation manager that lets you organize and cite papers. With the Zotero Connector, papers...managers. If you want more tips on finding and organizing papers, check out this related blog post: Early...Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out about Zotero here Dear...manuscripts: literature searching, reading, and organizing Tips for writing a scientific review article ... -
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...small, and their transparency allows for whole organism imaging. They created an endogenous ApoB-NanoLuc...excitable cells of the nematode Caenorhabditis elegans. They have found several voltage biosensors and...were larger than previously reported for other organisms, and the Gottschalk group hypothesizes that this...may be due to different lipid compositions of C. elegans membranes, which might beneficially influence the... -
CRISPR Plasmids - Bacteria
TypeCollection...Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR... -
TALEN Guide
TypeCollection...www.taleffectors.com) . Whether you work in a C. elegans lab and have been struggling to mutate a specific... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...showed that it can alter motor responses in C. elegans. Find the split BoTN/B plasmids at Addgene. Liu...in mammalian hosts Article contributed by Lukas Morgan Listen to the acoustic reporter gene podcast segment...the body. When a microbe travels through a host organism, the expression of some genes can change depending...protein structure that water-bound photosynthetic organisms use for buoyancy. These proteins allow dissolved... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post..., if you are studying a protein that may bind a ligand during your experiment, then you’d want to make...antibody that recognizes an epitope far from the ligand binding site (Mould et al., 2016). If your antibody...part of your protein that may be masked while the ligand is bound, then you’ll get a false negative signal...Byron A, Takada Y, Jowitt TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological ...