We narrowed to 396 results for: ICL
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TypeBlog Post...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid...
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15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post... for producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as...integration needed for the production of lentiviral particles. It’s capable of inducible expression of shRNA... -
Viral Vectors 101: Biosensors
TypeBlog Post...determine viral tropism—the type of cells the viral particles infect. This can direct your biosensor to specific...choose lentivirus, gamma retrovirus, or AAV as your vehicle, we’ve got you covered with packaging plasmids!... -
Viral Vectors 101: Types of viruses
TypeBlog Post...infect both dividing and non-dividing cells. Lenti particles enter the nucleus through nuclear pores and thus...Relative to the other viruses discussed in this article, adenovirus has the highest maximum titer, a plus... -
RNA Extraction Without A Kit
TypeBlog Post...solutions when all the spin columns run out. In this article, we cover the basics of isolating RNA without a...is to precipitate the RNA using lithium chloride. LiCl solutions selectively precipitate RNA, but not DNA... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...have the words to focus on. If you’re reading an article online, you have the words and maybe a few links...vying for your attention. If you’re reading an article with images, now you have words, images, and links... -
Transferable Skills Guide: Problem Solving
TypeBlog Post...transferable skills seemed fuzzy or ill-defined. In this article, I try to clarify what people mean when they ask... After sifting through dozens of career advice articles, I settled on defining problem solving skills ... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...materials and progress reports, in addition to articles for the Fred Hutch website, social channels and... says Susan. Another difficulty is that some articles are just harder to write. “There’s something about... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog Post...were at institutions where no data had been made publicly available (below). However, on November 22nd a...Resources on the Addgene Blog Read Gary's Previous Article: Changing Labor Laws Bring Increased Postdoc Wages... -
Hot Plasmids - October 2020
TypeBlog Post...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...You can learn more about their research in this article. To see other plasmids for SARS-CoV-2 spike pseudotyping...Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...the repository through our hot plasmids articles. These articles provide brief summaries of recent plasmid... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...contains the same sections as a primary research article, it’s important to understand that presenting your...Posters should not look like a primary research article condensed into one big page. Your sections should... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post...Viruses are intracellular parasites and natural vehicles for genetic information. Therefore they make excellent...produce a ~10-fold higher titer of lentiviral particles than the original lentiCRISPR v1. Special note... -
Viral Vectors 101: Chemogenetics
TypeBlog Post... and some, such as varenicline, are already approved for clinical use. Varenicline has been further modified... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog Post...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...popularity as they are uniquely suited gene delivery vehicles with great safety profiles as well as other benefits... -
Tips for Technical Support Calls
TypeBlog Post...thousands of depositors. That means their original articles are your best bet to figure out how to use a particular...using Google Scholar, which searches scientific articles, patents, and even some doctoral dissertations... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post... personality types is beyond the scope of this article, there are a few principles that will help you ...Management for Scientists" series More science career articles on our blog Read Joanne Kamen's Post on What ... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...methodologies have been extensively described in other articles; so if you are interested in learning more about...Plasmids 101 blog posts New to plasmids? Read our article on What is a Plasmid? Download the Plasmids 101... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog Post...sequence all the DNA packaged inside the viral particles (Figure 1). Briefly, packaged DNA is extracted...recombinase-independent recombination occurs in 0.1-0.6% of AAV particles in a given viral prep. With this in mind, we recommend...