We narrowed to 298 results for: IRES

1. Plasmids 101: Origin of Replication

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   Blog Post

   ...varies. See below for additional considerations. *Requires pir gene for replication (reference). **F1 is...

2. Summer SciComm: Science Storytelling

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   Blog Post

   ...opposing forces or (2) the result of competing desires or (3) the presence of obstacles that need to be...

3. Plasmids 101: TOPO Cloning

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   Blog Post

   ...does not use restriction enzymes or ligase, and requires no post-PCR procedures. Sounds easy right? The...

4. Trading Spaces - Visiting Scientist Edition

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   Blog Post

   ...from equipment sharing or a collaboration – it requires re-location of the scientist to the new institute...

5. The Power Behind NGS Plasmid Validation: seqWell

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   ...limited by costly library prep, as each sample requires an individual library. The plexWell™ workflow ...

6. Science Career Options

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   ... Experience in the field or in a position that requires travel can show that you are self organised, reliable...

7. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

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   Blog Post

   ...pointed out that networking at virtual conferences requires you to take more initiative. “You have to actively...

8. Visualizing Genomic Loci with CRISPR-Sirius

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   Blog Post

   ...different chromosomes in living cells, the system requires high-copy chromosome-specific loci in the genome...

9. Improving Transduction Efficiency with Exosome AAVs

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   ...delivery of therapeutic proteins to the brain which requires crossing the blood-brain-barrier. In summary, ...

10. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post

    ...transcription. Transcription from σ54 promoters requires binding of bacterial enhancer binding proteins...

11. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to recognize the target. Osamu Nureki...

12. Plasmids 101: NGS Quality Control for Pooled Libraries

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    ...a powerful research tool, but their proper use requires careful amplification and maintenance of good ...

13. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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    Blog Post

    ...longer exposure times. Additionally, KillerRed requires mitochondrial targeting to increase cell ablation...

14. 5 Great Apps for Lab Life

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    Blog Post

    .... The tools are a lifesaver when your research requires cell culture and include a counter, lab timer,...

15. From Lab Bench to Leadership: Transitioning into Management as a PhD

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    ... goals, and fostering team success. This shift requires deliberate preparation and a willingness to embrace...

16. Viral Vectors 101: The AAV Retrograde Serotype

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    Blog Post

    ...any genetic tool delivered by the AAV.   AAVrg requires high titers for efficient retrograde transport...

17. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post

    ...dividing cells, as their access to the host genome requires breakdown of the nuclear envelope, which occurs...

18. Hot Plasmids - February 2022

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    Blog Post

    ...label your favorite protein! While the target only requires a small epitope tag (FLAG is eight amino acids...

19. Antibodies 101: Isotypes

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    Blog Post

    ...deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene locus. ...

20. Luminescent Imaging with Nano-lanterns

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    Blog Post

    ...technology. Multicolor imaging using Nano-lanterns requires a linear unmixing algorithm to separate the various...