...sites, will result in similar sized bands, thus making this simple digest less informative. This is particularly...freezer and you are not 100% sure it is what you are looking for, but you have a map and know exactly what it...

...Liquid Bacterial Culture You may also like... Streaking and Isolating Bacteria on an LB Agar Plate Creating...Incubate bacterial culture at 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require...

...appropriate for the lab space in which you are working. Even if your samples may not require specific ...centrifuge is clean and that everything appears to be working smoothly. Place your sample tubes into the rotor...

... with a two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations... fine in an assembly if you want to save time. Working on ice, combine segments in the Gibson Assembly...

...amounts of liquid (think: 0.1 µL to 1 mL). When working in a laboratory, properly dispensing liquid ensures...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...

...syringe and a blunt edge 18 ga Hamilton needle, taking care to avoid bubbles (Figure 1). 8 mL of 15% iodixanol...facing the 40% step. Collect up to 5 mL per tube, taking care to avoid collecting the proteinaceous material...

...Wear a lab coat, eye protection and gloves when working with this chemical. Note: If you add EtBr to your...the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield, gloves...

...business of iPSC clone picking, a 100-fold decrease is the difference between picking 100 versus 10,000 clones...efficiency.   But what’s better: PAM-blocking or guide RNA blocking mutations? This depends entirely on...efficiency of guide RNA blocking mutations. So if the locus does not allow blocking the PAM sequence (e.g...intended mutation while taking advantage of the improved efficiency provided by blocking mutations. Essentially... incorporation of the blocking mutation. Despite the fact that both the blocking mutation and our intended... same CRISPR/Cas-blocking mutation but not the intended mutation. Here, the blocking-only templates will...used CRISPR/Cas9 extensively for the purpose of making sequence-specific changes in human induced pluripotent...

...experience working in a developing tropical country. This is why I set up a placement working in a rural...work-life balance compared to working in research. My previous experience working in Industry was really valuable...sustainable. After working at this company for several years, I was ready for a change.  Working abroad: Research...new science graduates and the pro’s and con’s of working in different sectors. Luckily, I have worked for... decide for themselves.  Here is my experience looking for the perfect job. Summer work experience in... explain why I changed jobs. I highly recommend taking on a similar position in your field of interest...and problem solving. I believe that is something lacking during degree courses; they never really prepare...

...insufficient blocking) or reduced signal (too much blocking.) If you’re having issues with your blocking, first...it, try making fresh running buffer. Old or improperly made running buffers can cause all kinds of odd ..., degraded or improperly mixed blocking buffer, or insufficient rocking during incubation steps.  Many...pattern in your background, try asking more experienced labmates or looking it up online. You’ll find many...ResearchGate’s western blot forum.  Blocking Don’t skimp on the blocking time! Fill the time with smaller...  Troubleshooting Blocking reduces the amount of background in your blot, so blocking issues can either...you can try either longer blocking times or higher concentrations of blocking buffer. If you see a low ...

...room, so it is a good idea to start thinking about public speaking early and often. Two of my jobs have...supposed to be done. They will start looking at their phones. Speaking longer just makes you seem unprepared...choosing controls, or mixing clear solutions—public speaking is skill that any scientist can learn.  Any time...how to get it, especially if introverted ), and networking (how to get people to remember you). What better...Watch other people give great talks We are not talking about lab meeting here. Lifeless presentation of... Series. Watch how these speakers talk without looking at their slides or notes. Listen to their stories...and policy content. Watch Joanne give her "Not" Networking 101 Talk 2. Tailor every talk to the occasion...

...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

...46 : How Kingdom ended up working in the life sciences and agriculture 5:47-9:35 : Kingdom's biotechnology...: Intro 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to...14:53 : Kingdom's current projects producing transgenic crops 14:54-23:44 : Follow up Kingdom's PhD projects...Seeding Labs is helping advance Kingdom's research 28:08-END : Kingdom's Thoughts on his time in Boston...family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best of the best...Biotechnology at Michigan State University with the backing of a prestigious Fulbright Fellowship. Dr. Kwapata...research at Michigan State University 9:36-10:29 : Kingdom's transition back to Malawi and Lilongwe University...

...presenting on building relationships (also known as “networking”), one of the most common questions I receive...starting to see the advantages of using social networking to start, build, and track professional relationships...professional contact information available. When I am speaking at a new institution, for example, I will often...d be much more likely to say  “Hey, thanks for looking me up on LinkedIn!” resulting in just a little ...inviting someone to connect, perhaps you should be asking yourself if it’s really a good idea.  Pro tip: ... unless you’re on a profile page. You might be looking at a list of suggested connections and you’ll see...complete strangers as it borders on my first rule of networking--”If it feels creepy, don’t do it.” It’s just...

...chat box and upvote If the thought of walking up to the mic and asking your question in a large conference...in case someone’s internet cut out. “ On networking Networking can be more difficult in the virtual format...organic networking,” she says.Depending on the meeting, there may be a “place” for networking within the...answered this way and the hurdle of getting up and asking in front of many people is lower,” says Bachle....,” says Zhang. “Usually the moderator starts by asking the speaker the highly upvoted question.”If the...in the chat box. That’s another benefit to note-taking: you can revisit the Q&A in the chat box. Presenting...the participants to learn about what they were looking to learn in the workshop so they could steer their...

...scientist who began working remotely recently? Most of us at Addgene began working from home last week... mindfulness group is sticking with their Headspace practice even while working remotely. Social distancing...week to flatten the curve, but working remotely can be more difficult if your work is based in the lab. ...sharing links to online yoga classes. Get outdoors. Taking a walk or a bike ride is a great activity for both...the desire and ability to, some communities are looking for bench scientists to assist in labs that are...volunteering to foster a pet, for example. If you are looking for some resources specific to self-care during...check out Care For Your Coronavirus Anxiety and Taking Care of Your Behavioral Health. Work on personal...

... to DNA, crosslinking may not be necessary. This is known as Native ChIP.  If you’re working with a tissue...analysis process. And ChIP itself begins at...  Crosslinking Once you have your sample, the first step is...sample processing and therefore will require crosslinking, which uses formaldehyde or UV light to create...complexes together until you want to break them. Crosslinking is necessary for most ChIP experiences, but ...determine concentration.  Protein Capture After crosslinking and sonication or enzymatic digestion, you’re... not be a good choice) and the amount of cross-linking can affect epitope availability.  Collect the DNA...then incubate with Proteinase K to reverse the crosslinking. Finally, purify your DNA with a PCR purification...

...time for me to start considering my future path. Talking to Addgenies has helped me get a much better idea... Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since he... really get me where I haven’t gotten myself.” Perkins noted that there is a more emphasis today about...those doors to be opened.” Addgenie Eric Perkins presented a poster a few years ago at a CRISPR ...away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community college when she...assistant in a lab at the university that I went to. Making that switch from being a grad student to an employee...Technician, dreamed of becoming a doctor. After taking a year break in between undergraduate school and...

...there's a big office vs. lab culture shock). I'm looking to see if there are any resources that can help...and many of my scientist colleagues have as well. Making the transition from the bench can be jarring for...aren’t a lot of resources out there for scientists making this transition. In this installment of the Addgene.... What causes the lab → office culture shock? Working in an academic research lab involves a few key ...your family. I talked to a graduate student at a networking event recently who referred to her lab as her...applicant asked if she would partake in “critical thinking” in the position to which she was applying. I ... stretch of time that doesn’t involve critical thinking. Former Addgenie Jessica Welch says, “[It] seems...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where... cell culture, I would often respond to friends asking me to dinner saying something like, “I’ll be there...ve learned a lot since my days as an undergrad working with cancer cell lines. As I started planning out... this post useful. After a bit of research and thinking about my own processes, I’ve broken down time ...until it was too late. I didn’t spend enough time thinking about the possibilities and how to prioritize ...the day, month, or even the year, you should be thinking about two main things: How useful the task is...time it will take you to complete the task When picking which tasks to actually do, you should err on the...