We narrowed to 526 results for: MEA;
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TypeBlog Post...times A red flame means a plasmid has been requested 50+ times A blue flame means a plasmid has been...
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Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog Post... protein shell that excludes water but is gas permeable. These vesicles are primarily used by water-dwelling... of arg1 and arg2 ultrasound signals. This also means that multiplexing is possible if bacteria are sequentially... -
Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post... 1 out of 10,000 cells on average! Without some means of quickly determining which cells successfully ...Polymyxin B polypeptide Alters outer membrane permeability Bactericidal 10-100 ug/mL Tetracycline tetracyclin... -
Intro to Our Summer SciComm Series!
TypeBlog Post...other uses. Many laypeople would use “PCR test” to mean “a test that uses a biological sample to look for...for presence of a virus.” A scientist would likely mean “a test for the presence or absence of a predefined... -
New and Upcoming Viral Vectors - December 2019
TypeBlog Post...spatiotemporal resolution required for precise in vivo measurements of dopamine (113049, 113050) or norepinephrine...Looger’s lab at Janelia for neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog Post...temperature readings using a heat gun, and pH measurements. We collected small water and sediment samples...samples and move farther away until the bison meandered off. In the end we were able to safely get back... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...toxic,” Merkx explained. New tools were needed to measure them. Merkx says, while in principle adapting a...them,” Merkx said. “For us, it is one of the main measures of success – whether we have really developed ... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog Post...poster (Reshef et al., 2020).The Twitter format meant that the posters could be viewed publicly and stored... difficulties? Attending a conference virtually means getting to know the app or virtual platform and ... -
Visualizing Genomic Loci with CRISPR-Sirius
TypeBlog Post...interest, the team could visualize three loci and measure inter-locus distances. They tested this system ...intergenic DNA region (IDR3) as a reference loci to measure inter-locus distances of loci pairs from intergenic... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...deletions on biofilm formation and growth will then be measured to link the deleted genes to specific functions...river, take water samples at specific locations, measure fluorescent signals from the sensor, and send a... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...multiplexed pool. The cost of these normalization steps, measured in time and consumables, can be daunting for large...normalized to two-fold variation above and below mean read count per sample, and the amount of information... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...process of generating your own fly lines. In the meantime, be sure to browse the publication references ...Brand, N. Perrimon, Targeted gene expression as a means of altering cell fates and generating dominant phenotypes... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...combinations that can be imaged together, but this also means that the choice of fluorescent proteins requires...this filter set and have reported brightness measurements. Here, EGFP outperforms the improved folding... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...plasmid components, it may be useful to describe the means of introducing genetic material (such as plasmids... post, we will define positive selection as the means of picking up positive traits (i.e. the plasmid ... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...respond as expected, use of representative images, measuring the main outcomes in several different ways, and...and you think there are four important times to measure that growth rate, you report the results of all... -
Developing a cold tolerant E. coli using specialized chaperones
TypeBlog Post... for milk processing, fruit juice clarification, meat tendering, and oligosaccharide synthesis. A major...function well under suboptimal conditions. This means recombinant proteins can aggregate or precipitate... -
Viral Vectors 101: Types of viruses
TypeBlog Post...generally have moderate to high immunogenicity, meaning they induce an immune response in the host. So...endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the same high immune response... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post... yellow GECIs, OCaMP shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater...curves for OCaMP and various red and yellow GECIs measured with soluble protein. Reproduced from Aggarwal... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post... commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria can easily...electrical charge to cells, to increase cell membrane permeability and thus transformation efficiency. Check out... -
Targeted Mutagenesis with EvolvR
TypeBlog Post...generating mutant libraries exist, but none provide a means to continuously diversify all nucleotides within...size at the target site. The use of such libraries means that the diversity introduced by lambda red recombineering...