We narrowed to 252 results for: Mac

1. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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   ...lysogenic. Lytic phages hijack the bacterial hosts machinery to make more viral particles. Eventually the cell...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...efficient gene deletion in B cells, T cells, macrophages, and dendritic cells, with no deleterious effects...

3. Clear and Accessible Writing

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   ...through increasingly dense layers until the macromolecule density is equal to the buoyant force of the...

4. GCE4All: Making Genetic Code Expansion Accessible

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   ...non-interacting with) the cells’ own natural translation machinery; these are often incorporated into the cells via...

5. Viral Vectors 101: Viruses as Biological Tools

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   ...associations: common colds, COVID-19, norovirus (the stomach flu), and many more. Their infectious nature allows...

6. Sharing is Caring: But How to Distribute Open Hardware?

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   ...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation...

7. GPCRs: How Do They Work and How Do We Study Them?

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   ...some GPCRs further to respond to specific, pharmacologically-inert, small molecules. These new receptors...

8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   ...provides greater spatiotemporal resolution than pharmacological or genetic manipulation of the signaling pathway...

9. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   ...podcast segment As the primary cause for both the stomach flu and food-borne illnesses, noroviruses can infect...

10. CRISPR 101: Non-Homologous End Joining

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    ..., repressing RecA and overexpressing the NHEJ machinery improved NHEJ accuracy in M. smegmatis (Yan et...

11. RNA Extraction Without A Kit

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    ...0005-2744(74)90240-x Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically...

12. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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    ...10.1073/pnas.0700293104 Field J, Nikawa J, Broek D, MacDonald B, Rodgers L, Wilson IA, Lerner RA, Wigler M (...

13. Human Germline Editing Using CRISPR

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    ...circumvent this problem, they co-injected CRISPR machinery with sperm into M phase oocytes, thus allowing...

14. Lighting Up Cell Signaling with Photoswitchable Kinases

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    ...provide greater spatiotemporal resolution than pharmacological or genetic approaches, but only a few such ...

15. Viral Vectors 101: An Introduction to AAV

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    ...expression AAVs have to infect cells, hijack host cell machinery, and synthesize a second strand of DNA before ...

16. All in a Twist: dsRNA

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    ...foreign dsRNA activates the innate immune response machinery of the host. This pathway has been clinically...

17. Giving gRNAs a Facelift - Synthetic and Beyond

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    ...samples. RNA is inherently less stable than most macromolecules used in scientific research such as DNA or ...

18. Working with Nuclear Receptors

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    ...physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity purification...

19. How to Prepare for an Industry Interview

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    ...ago. I just recently started working in the pharmaceuticals industry after earning my Master’s in 2016 ...

20. Popular Retroviral Vectors and Their Uses in Scientific Research

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    ...can express siRNAs to direct the cellular RNAi machinery to silence target genes. This empty MuLV vector...