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We narrowed to 420 results for: Numb

Showing: 161 - 180 of 420 results

  1. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...genome-containing viral particles relative to the total number of viral capsids (which can include empty capsids...PCR system for absolute quantitation of DNA copy number." Analytical chemistry 83.22 (2011): 8604-8610.4...

  2. Lab to Office Culture Shock

    Type
    Blog Post
    ...transition. Of course, a “desk job” could entail any number of career paths--industry, nonprofit organization...alternative careers.” Realistically, based on the numbers of PhDs and available positions alone, it’s the...

  3. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...is reproducible, and only the highly restricted number of people who can access the ELN have the ability... data repositories Over the past decade or so a number of general purpose repositories have been launched...

  4. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...within a given sample is simply determined by the number of bands present on the strip. This type of readout...provide a CRISPR-based platform on which an infinite number of tests” for biosensing can be built upon. Mammoth...

  5. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...time called sprints. The team is mindful of the number of stories we commit to for each sprint, to ensure...the feature. For example, we recently reduced the number of steps involved during checkout. Demo details...

  7. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type
    Blog Post
    ... be unstable and lack precise control over copy number. MUCICAT system contains 4 plasmids: pDonor, pTnsABC...transposition-inducing agar lead to higher cargo copy numbers. (D) The effect of gene dosage on N-acetylglucosamine...

  8. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ..., according to the NSF data) and therefore the numbers of postdocs at different points on this salary ... issues. In the long term, it seems likely the number of postdoc positions in the biomedical enterprise...

  9. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...culture post thaw. In general, the higher the passage number of a culture the lower the transfection and transduction...with other envelope proteins may have a limited number of targets and therefore titers obtained in one...

  12. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...consuming and expensive process. Consider the vast number of possible primary antibodies that exist and you...your protein of interest. Thankfully, there are a number of websites compiling antibody data that can help...

  14. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...expression significantly reduced the HIV-1 copy number, although the efficiency differed from 48-100% ...similar experiment, CRISPR reduced the HIV-1 copy number by over 50% in isolated CD4+ T-cells from two HIV...

  15. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...for an individual application is dependent on a number of factors, including species, strain, age, sex...off-target expression, since they are “seeing” a great number of cells as they circulate in the blood, rather...1011 vg/mouse), AAV-PHP.eB can transduce a greater number of neurons in the CNS (Chan et al., 2017). AAV-...

  16. Antibodies 101: Validation

    Type
    Blog Post
    ...Bradbury & Plükthorn, 2015).  To combat this, a number of groups have come together over the past few ... Strategies for Antibody Validation There are a number of resources available that provide in-depth discussions...

  17. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...they make a copy of themselves. This increases the number of copies of the TE while also increasing the size...TTAA for Sleeping Beauty, they can insert at any number of these sites found in the genome.  To target ...

  19. Deep Dive: qPCR

    Type
    Blog Post
    ...you run the plate.    Analyzing There are a number of methods available for analyzing qPCR data, most...Shaun S. LightCycler qPCR optimisation for low copy number target DNA. Journal of Immunological Methods 270...

  20. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...etc.) can effect reprogramming rates and such a number maybe misleading. Integrating iPSC delivery methods...robust and efficient generation of iPSCs is the number one priority, with safety taking lower priority...
Showing: 161 - 180 of 420 results