Antibodies 101: Flow Cytometry Controls
Type
Blog Post
...name suggests, a single color from your antibody panel. They are made up of compensation beads and a conjugated...for every fluorophore you intend to use in your panel.
Using single color controls
On a flow cytometer... the reference plots provided by the antibody companies or check the literature.
Pro tip! Early gates,...consider using an isotype control.
Pro tip! Many companies will have appropriate isotype controls linked ...