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We narrowed to 538 results for: PAN

Showing: 161 - 180 of 538 results

  1. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...training, I was gradually introduced to Addgene as a company and given more details on what my position entailed.... The official training process was lined out to span the first three months of my six month employment...and Geneious, I was able to determine if any discrepancies between what we sequenced in house and the submitted...they do and how it relates to other teams at the company. This provides new (and old) employees with information...

  2. RUBY-Red Siliques

    Type
    Blog Post
    ...Bottom panel, right) A highly expressing RUBY plant is a vibrant red/magenta while (bottom panel, left)...plant beginning to germinate. (Top right, second panel) Transformed seedlings can be selected without antibiotics...developing seeds from a homozygous RUBY plant. (Bottom panels) RUBY expression can vary from different transformation...

  3. Virtual Science Conference Coming Up? Three Addgenies Share Their Experience

    Type
    Blog Post
    ...first sent a pre-workshop questionnaire to the participants to learn about what they were looking to learn... content. In the middle of the workshop, the participants took part in a protocol drawing exercise. The...workshop also used a shared note-taking doc where participants could share their notes together. Figure...viewed publicly and stored in one place if the participants used the conference hashtag after the conference...

  4. How to Lead a Great Meeting

    Type
    Blog Post
    ...collaboration - a sense of dialogue and community among participants, clarification - new and useful information,... if any additional resources are needed. All-Company Meetings: As your organization grows, so will the...to address one point aloud. This keeps our all-company meetings short, informative, and pleasant. Email...timely feedback on each other’s projects. For all-company meetings, monthly still works best. Figure out ...

  5. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...name suggests, a single color from your antibody panel. They are made up of compensation beads and a conjugated...for every fluorophore you intend to use in your panel. Using single color controls On a flow cytometer... the reference plots provided by the antibody companies or check the literature. Pro tip! Early gates,...consider using an isotype control. Pro tip! Many companies will have appropriate isotype controls linked ...

  6. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...hardware. Upper left panel shows the path of light through a microscope. Lower left panel shows an image of...and can allow for innovative organizations and companies to provide problem-solving platforms.     ... monocyte with planes and hardware to it. Right panel shows workflow of open hardware.    Conclusion...

  7. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...other luciferases have been identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus...plagiophthalamus), deep-sea copepod (Gaussia princeps), Japanese firefly (Luciola cruciata), and more. These variant...or codon-optimized 562 61 Luciferin ATP No Sea pansy (Renilla reniformis) Natural 481 36 Coelenterazine...princeps) Natural 480 20 Coelenterazine None Yes Japanese firefly(Luciola cruciata) Natural 562 64 Luciferin...

  8. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...repurposed for SARS-CoV-2 RNA detection. Early in the pandemic, labs around the world have been modifying these...viral proteins In the early weeks of the COVID-19 pandemic, the Krogan lab at UCSF generated lentiviral vectors... while many were developed during the COVID-19 pandemic. Our mission to accelerate research through sharing... up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2 pseudovirus plasmids ...

  9. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman and...advocacy skills “bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org...productive routes for advocacy efforts and help participants develop the efficacy of their advocacy skills...effective advocacy strategies on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor...

  10. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...each year. (The dip in 2020 is likely due to the pandemic; for more discussion on that, see Pyhtila et al...CRISPR plasmids. But many other countries, from Japan to Australia, are significant depositors and requestors...CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse..., S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse...

  11. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... are already reserved for other markers in your panel, and thus cannot be used, you may want to look at...flexibility when it comes to fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids...advantage of this system is increased flexibility for panel design. Another perk is that one primary antibody... be different from the other antibodies in your panel. Otherwise, your secondary antibody will bind to...

  12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells Note: This is not an extensive...Cy dye-nitrilotriacetic acid (NTA) conjugates (Kapanidis, Ebright, & Ebright, 2001). These Cy/NTA conjugates... et al. "Designing logical codon reassignment–Expanding the chemistry in biology." Chemical Science 6.1...Science281.5374 (1998): 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. ...

  13. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...AAV Packaged on Request, an expansion of our viral vectors service, has officially launched! As of today... excited for all the ways this much-requested expansion will allow us to better serve our community. If...If you’re curious about the service, read on.  Expanding our viral vector service Addgene has offered in-stock...

  14. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...learning to navigate the changes brought about by the pandemic. As we adjust, we are reflecting on how to help...information sharing, and research reproducibility while expanding our suite of materials and services to support...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...

  15. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...COVID-19 research. With the COVID-19 pandemic, we saw the need to expand reagent sharing even broader and ...Tsang These tips were written early on in the pandemic. Later in the year, Joanne Kamens followed up ...

  16. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...As the world has battled the COVID-19 pandemic over the past few months, the scientific community has...research. The new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not...care and public health for this current COVID-19 pandemic, and outbreaks that have yet to come,” said the...

  17. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...Expanding your network of relationships early and often is the most effective tactic a scientist in training... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ...

  18. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ... Society, or maybe you think about a local food pantry or community arts organization. Many people are...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...physiology, development, and disease. In order to expand their model’s impact, organoid users have recently...months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9... INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON...

  20. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...other disciplines, over time. The resource may also expand to include other affinity reagents, such as nanobodies...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...
Showing: 161 - 180 of 538 results