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We narrowed to 535 results for: PAN

Showing: 161 - 180 of 535 results
  1. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...name suggests, a single color from your antibody panel. They are made up of compensation beads and a conjugated...for every fluorophore you intend to use in your panel. Using single color controls On a flow cytometer... the reference plots provided by the antibody companies or check the literature. Pro tip! Early gates,...consider using an isotype control. Pro tip! Many companies will have appropriate isotype controls linked ...
  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...hardware. Upper left panel shows the path of light through a microscope. Lower left panel shows an image of...and can allow for innovative organizations and companies to provide problem-solving platforms.     ... monocyte with planes and hardware to it. Right panel shows workflow of open hardware.    Conclusion...
  3. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...other luciferases have been identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus...plagiophthalamus), deep-sea copepod (Gaussia princeps), Japanese firefly (Luciola cruciata), and more. These variant...or codon-optimized 562 61 Luciferin ATP No Sea pansy (Renilla reniformis) Natural 481 36 Coelenterazine...princeps) Natural 480 20 Coelenterazine None Yes Japanese firefly(Luciola cruciata) Natural 562 64 Luciferin...
  4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...repurposed for SARS-CoV-2 RNA detection. Early in the pandemic, labs around the world have been modifying these...viral proteins In the early weeks of the COVID-19 pandemic, the Krogan lab at UCSF generated lentiviral vectors... while many were developed during the COVID-19 pandemic. Our mission to accelerate research through sharing... up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2 pseudovirus plasmids ...
  5. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman and...advocacy skills “bootcamp” for a focused group of participants. More information can be found at http://futureofresearch.org...productive routes for advocacy efforts and help participants develop the efficacy of their advocacy skills...effective advocacy strategies on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor...
  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...each year. (The dip in 2020 is likely due to the pandemic; for more discussion on that, see Pyhtila et al...CRISPR plasmids. But many other countries, from Japan to Australia, are significant depositors and requestors...CRISPR data? Read our recent publication, “The Expanding Dissemination and Distribution Patterns of Diverse..., S., Leeson, R., & Barrangou, R. (2023). The Expanding Dissemination and Distribution Patterns of Diverse...
  7. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... are already reserved for other markers in your panel, and thus cannot be used, you may want to look at...flexibility when it comes to fluorophore selection for panel design. Find streptavidin plasmids and biotin plasmids...advantage of this system is increased flexibility for panel design. Another perk is that one primary antibody... be different from the other antibodies in your panel. Otherwise, your secondary antibody will bind to...
  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells Note: This is not an extensive...Cy dye-nitrilotriacetic acid (NTA) conjugates (Kapanidis, Ebright, & Ebright, 2001). These Cy/NTA conjugates... et al. "Designing logical codon reassignment–Expanding the chemistry in biology." Chemical Science 6.1...Science281.5374 (1998): 269-272. PubMed PMID: 9657724. 6. Kapanidis, Achillefs N., Yon W. Ebright, and Richard H. ...
  9. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...AAV Packaged on Request, an expansion of our viral vectors service, has officially launched! As of today... excited for all the ways this much-requested expansion will allow us to better serve our community. If...If you’re curious about the service, read on.  Expanding our viral vector service Addgene has offered in-stock...
  10. 2022 and 2023: Reflections and Looking Forward

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    Blog Post
    ...learning to navigate the changes brought about by the pandemic. As we adjust, we are reflecting on how to help...information sharing, and research reproducibility while expanding our suite of materials and services to support...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...
  11. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...COVID-19 research. With the COVID-19 pandemic, we saw the need to expand reagent sharing even broader and ...Tsang These tips were written early on in the pandemic. Later in the year, Joanne Kamens followed up ...
  12. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    ...As the world has battled the COVID-19 pandemic over the past few months, the scientific community has...research. The new Center for COVID-19 Response and Pandemic Preparedness (CCRP2) at Rutgers University not...care and public health for this current COVID-19 pandemic, and outbreaks that have yet to come,” said the...
  13. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...Expanding your network of relationships early and often is the most effective tactic a scientist in training... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ...
  14. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ... Society, or maybe you think about a local food pantry or community arts organization. Many people are...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better...
  15. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...physiology, development, and disease. In order to expand their model’s impact, organoid users have recently...months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9... INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON...
  16. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...for light mediated neuronal control and we are expanding our viral service to make these readily available... experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes... is vital in studying neural networks. We have expanded our calcium sensor collection to include our first...
  17. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    ...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...other disciplines, over time. The resource may also expand to include other affinity reagents, such as nanobodies...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...
  18. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...build and grow the gene therapy division of the company.  Ultragenyx’s goal is to develop novel products...annotated reagent.” He’s also interested to see Addgene expand the sharing platform to other reagents in biology...grants, or setting up commercial and nonprofit companies. His lab focuses on gene therapy work for genetic...
  19. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...career?”, "Have they sought out opportunities to expand their skill sets?", “Have they succeeded in what...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...
  20. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...commonly used yeast by biotech and pharmaceutical companies is K. phaffii (formerly known as pichia). What...general research community. Phillips Petroleum Company first commercially developed K. phaffii in the ...Since then, multiple biotech and pharmaceutical companies (along with some research laboratories) have picked...
Showing: 161 - 180 of 535 results