We narrowed to 257 results for: PORA

1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

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   Blog Post

   ...the deletion. REPLACR-mutagenesis successfully incorporated a 60-nucleotide insertion; beyond this insertion...

2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   Blog Post

   ...sequence flexibility indicates that gRNAs could be incorporated into these constructs without compromising cleavage...

3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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   Blog Post

   ...bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics, Inc. in March 2009...

4. Kiran Musunuru on the Newest TALEN Genome-Editing System

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   Blog Post

   ...plasmids have been introduced by transfection or electroporation. All you do to create a protein that binds ...

5. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   Blog Post

   ... Eisenhoffer lab, is a new tool that increases temporal and spatial precision of gene expression and reduces...

6. Celebrating 15 Years of Scientific Sharing

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   Blog Post

   ...robust committee process in place and continue to incorporate additional Board best practices. I thank them...

7. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   ...promoters that are always on, stabilized promoters incorporate feedback loops such as the incoherent feedforward...

8. RaPID Detection of RNA-protein Interactions

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   Blog Post

   ...interacting proteins, which results in poor spatiotemporal resolution for in vivo work. APEX2, a faster...

9. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   Blog Post

   ...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...

10. Human Germline Editing Using CRISPR

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    Blog Post

    ...containing Cas9 and two different gRNAs, and electroporated them with an ssODN repair template in induced...

11. Lighting Up Cell Signaling with Photoswitchable Kinases

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    Blog Post

    ...control of kinase activity can provide greater spatiotemporal resolution than pharmacological or genetic ...

12. To Each HIS Own

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    Blog Post

    ...tended to be very large and often required the incorporation of a cleavage site and a post-purification cleavage...

13. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...different ways depending on the team. When Tony, our corporate counsel, used the word “service,” did he mean ...

14. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    ...pair to another base pair at a specific site. 2. Temporal, spatial, or conditional control of CRISPR activity...

15. Design Tips for Prime Editing

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    Blog Post

    ... cellular surveillance. To limit unintended incorporation of the pegRNA scaffold sequence into the genome...

16. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

17. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    Blog Post

    ...Hybridization (WISH) WISH is a technique that detects the temporal and spatial localization of mRNA transcripts in...

18. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ... is particularly interested in strategies to incorporate research into undergraduate courses and in communicating...

19. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    Blog Post

    ...while also capturing information about spatial and temporal organization. The design use to detect PKA activity...

20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

    ...HRP-TMB reaction more quickly. Just make sure to incorporate the dilution factor in your calculation from ...