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We narrowed to 653 results for: PPL

Showing: 161 - 180 of 653 results

  1. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...well as suitable applications. Expression System Components of System Application Mammalian expression...CRISPR mammalian expression systems, the typical applications for each, and potential delivery methods.   ...Select the appropriate CRISPR tools for your application: Wildtype Cas9 or Cas9 nickase are appropriate...or more sgRNAs and Cas9 are required for any application. If you want to include a homology directed repair...

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...improvements to CRISPR technology since its initial applications for genome engineering in 2012.  Many of these...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed...allowing DNA binding and cleavage. This technique was applied to create photoswitchable spCas9, dCas9, and S...., allowing the release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated...Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced Dimerization ...continue to evolve and can’t wait to see what cool applications you use these for in the future! References...

  3. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ... and are ready to move on to your downstream application — but wait! Do you know how much plasmid DNA ...reading results from a NanoDrop. The principles will apply to similar instruments from other companies, like...sample will have minimal impact on downstream applications, but they can impair your ability to get an ...be sure it is compatible with your downstream applications and that you use the same solution for blanking...A230 ratios in RNA preparations on downstream applications. Qiagen Gene Expression Newsletter. Issue 15...

  4. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...production system may depend on what downstream applications you are planning, cost, and the amount of protein...accurate concentration to give your downstream applications the best chance at being successful.  Using ...they bind their antigens specifically. Possible applications for validation include western blot, immunohistochemistry...some antibodies are better suited for certain applications. An antibody that performs well in immunohistochemistry...use! You can learn more about common antibody applications in our Antibodies 101 blog series.  Resources...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...their experimental needs, and some of the common applications in the lab. Why Green Fluorescent Protein?...Re-engineering GFP to increase its range of color and application Almost as soon as its sequence was elucidated...the mutation definitions found in Table 2 may not apply. Table 1: The specific mutations comprising common...others) Neutral mutations    A multitude of applications Due to its size and ease of use, GFP and other... just scratched the surface of the potential applications for GFP. It has also been used to identify particular...

  6. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...DNA into the genome is useful for a variety of applications. For example, specific tissues such as blood...pathways control this process. Additional zebrafish applications and conclusions Zebrafish are also popular outside...question, and zebrafish are not ideal for every application. Disadvantages include their duplicated (i.e....—have proven useful in a variety of research applications. Lineage tracing studies highlight how these...Contemporary zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments." Methods...

  7. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...use, functional in most species, and has many applications. That said, SpCas9 is not the only game in town... makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a: An RNA cleaving...discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   Figure 1: Using Cas13a as...of nucleotides.   What are the potential applications of Cas13a given what we know about its structure...CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was originally published...

  8. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...so much better. Driven by that, I successfully applied and worked as a technical support specialist for...expertise in technical support positions. When applying for technical support positions, you should try...company. To increase your chances at successfully applying for a position, you should familiarize yourself...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...

  9. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...decades. Widely used across various research applications, they have become indispensable tools for manipulating...-lasting changes in cells. However, for some applications, integration might be considered a double-edged... certain types of clinical or basic research applications. Considerations for IDLV use If you’re reading...Integrase-defective lentiviral vectors: progress and applications. Gene Therapy, 17(2), 150–157. https://doi.org...lentiviral vector: prospects for safe clinical applications. PeerJ, 10, e13704. https://doi.org/10.7717/...

  10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...performed on one register at a time. If a particular application makes it difficult to isolate single registers...state read-out method may not be suitable for applications that require minimal cellular perturbation. ...contains the register. Depending on the desired application, the inducible promoters we used to drive recombinase...program to find the one that best suits your application. How efficiently do RSMs work? We tested a two-input...anticipate that, depending on the context and application, some inducible recombinase systems may work ...

  11. Finding Your Science Policy Path

    Type
    Blog Post
    ...pursue your interests in policy or advocacy as a supplement to your career through engagement with your local...Project for some example proposals and consider applying for their accelerator for some hands-on guidance...This may be more challenging if you are trying to apply for an outright job in a field where you have no...specific scientific credentials.  But before you even apply to that first science policy job or fellowship, ...connect you with alumni before and during your application process so you can learn more! Figure ...

  12. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

  13. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...suitability of a systemic capsid for an individual application is dependent on a number of factors, including...test your chosen capsid before using it in an application.     Figure 1: AAV toolkit for targeted...doses are recommended for all systemic capsid applications. For example, AAV-PHP.eB is typically administered...experimentally determine the best titer for your application.   Pro tip!Want to know if a capsid is a good...always, validate the capsid for your specific application and animal model.  Other model organisms Tropism...points to its potential utility in translational applications and in models such as human organoids. Now what...helps you find the right one to test in your application. If you think you’ve found a good fit (or if ...

  14. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...scientific aspects of writing. Following his advice, I applied to the class. Receiving the email that I had been...the ASM headquarters. I decided to go ahead and apply; thinking I probably would not get selected for ...knowledge with practical understanding of how to apply it. The personal interaction of critiquing others...well as being critiqued added an extra level of application not present in the online class.   Four ways ...

  15. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in... same species as your primary/sample, and then applying this mixture to your tissue (Tuson, et al., 1990...After washing away unbound fragments, you can then apply your primary and secondary antibodies as normal....Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...

  16. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... solutions that facilitate most experimental applications in CRISPR engineering. When compared to that...easy to fault the lack of features in a single application. The question is then, should we use all tools...categories. On the one hand we have software tools that apply some approximate or sensible rules to determine ...This often results in software design that is applicable to only one, or a subset of organisms. In only...ProtospacerWB was developed with the purpose of applying CRISPR technologies to new organisms and will ...

  17. Hot Plasmids - January 2023

    Type
    Blog Post
    ...antibodies may just not perform as well in one application as another, so it is always best to have options...for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP [N86/8R] page for more...fluorescent proteins (FPs) necessary for many of these applications have lagged. Fixation, chemical staining, and...well-suited for the demands of advanced microscopy applications such as super resolution, electron, and expansion...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... our knowledge. Disease Applications Q13: Does AAV have potential applications in diseases which may be...1x1014 vg/ml.. As evident in our repository, this application is an outlier for PHP.eB (1x1011 - 1x1012 vg/...CRISPR tools is an exciting area for future AAV application to epigenetic editing. This area is discussed...

  19. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...spCas9 was the only Cas enzyme widely available and applied by researchers for gene targeting. Fast forward...properties, previously established uses, and intended applications. Within CasPEDIA, there is a classification ...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ...understanding and technical proficiency with common applications - now let’s dive right in! You know qPCR is...as it solves, so understanding and using the application correctly is imperative. Picking your chemistry... more, take a look at their 551 page e-book on applying MIQE guidelines! With these kinds of resources...melting profiles of PCR products in a rich web application, Bioinformatics, Volume 27, Issue 7, 1 April ...Solinas A; Booth J; Brown T. Mode of action and application of Scorpion primers to mutation detection, Nucleic...
Showing: 161 - 180 of 653 results