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We narrowed to 651 results for: PPL

Showing: 161 - 180 of 651 results

  1. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...information they’ve absorbed if they don’t get a chance apply it in an interactive setting. The problem is…how... as I try being compelling, interactive, and application-driven to boost curiosity, I have never experienced...they would use their logic and strategy skills to apply those concepts and understand how therapeutic strategies... BUILD YOUR PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology...opposing team. In the end, the students had fun while applying most of the concepts required by the course and...

  2. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    ...research box, send out some feelers, apply to programs, and apply for travel grants. Good luck and happy...visiting positions within defined labs which you can apply to. These structured types of placements often have...are your options if neither of these situations apply to you? Once you’ve identified one or more labs ...mentor can help get the ball rolling! If you are applying to a structured program, they likely have guidelines...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ...together a grant application? Are you familiar with the publishing or patent application processes? How ...How can you stand out among all the candidates applying for the position?   Here are a few things to consider...makes sense for your skills, and the job you are applying for. Also remember that a resume is typically ...have done is closely related to the job you are applying for, in which case talk about your research, as...

  4. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...with slightly lower quality preps, while other applications require large amounts of high quality DNA. Figure... a High Quantity/Low Quality mindset for any applications. DNA quantity is fairly self explanatory and...settings, but when it comes to low-throughput applications, the choice is largely a matter of preference... 96-well plate options for medium-throughput applications. For high-throughput options, several manufacturers...

  5. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...and deliver any cargo you fancy (size limits do apply, however.) Why use a virus? Viruses are essentially...low immunogenicity which makes them ideal for applications with whole organisms. Finally, viral delivery...delivery is typically straightforward across applications, while electroporation of a human, for example, isn... (~4.5kb From basic science to clinical applications, viruses are true bench to bedside tools. Keep...our upcoming blogs on virus safety and viral applications to help you start (safely!) thinking about the...

  6. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...definition of “problem solving skills” is the ability to apply the scientific method to problems in a variety of...strong problem solving skills is just a matter of applying that framework to the myriad challenges we encounter...ask them to explain their logic. Or you can try applying the scientific method to everyday problems in ...to practice problem solving skills and learn to apply them flexibly in a range of contexts. As such, scientists...remember that you have these skills when you start applying and interviewing for new positions. Highlighting...

  7. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...population. While the EnvA genetic sequences are supplied during production of EnvA-pseudotyped RABV, these...will not necessarily express EnvA unless it is supplied in trans. Monosynaptic RABV spread can be achieved...transsynaptic spread can be controlled by selectively supplying the G protein in trans only to starter cells (...replication will then occur and the G protein (supplied in trans) will be incorporated into the viral ...Wickersham et al., 2007).   Summary and RABV applications Use of G deleted RABV in conjunction with restricted...

  8. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...review how these indicators work, their general applications, and why viruses are essential to their use ...improvement. These sensors still have many useful applications, though, and if you’re in the market for a VSD...tissues depths (1 mm), important for certain applications such as in vivo tissue imaging.   Figure...your cell type of interest. For in vivo imaging applications, the ability to direct your voltage sensor selectively...genetically encoded voltage indicators advance their applications in neuroscience. Curr Opin Biomed Eng. 2019....

  9. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...well as suitable applications. Expression System Components of System Application Mammalian expression...CRISPR mammalian expression systems, the typical applications for each, and potential delivery methods.   ...Select the appropriate CRISPR tools for your application: Wildtype Cas9 or Cas9 nickase are appropriate...or more sgRNAs and Cas9 are required for any application. If you want to include a homology directed repair...

  10. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...improvements to CRISPR technology since its initial applications for genome engineering in 2012.  Many of these...particular interest since blue light has limited applications in vivo. UV and blue light is readily absorbed...allowing DNA binding and cleavage. This technique was applied to create photoswitchable spCas9, dCas9, and S...., allowing the release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated...Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced Dimerization ...continue to evolve and can’t wait to see what cool applications you use these for in the future! References...

  11. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...production system may depend on what downstream applications you are planning, cost, and the amount of protein...accurate concentration to give your downstream applications the best chance at being successful.  Using ...they bind their antigens specifically. Possible applications for validation include western blot, immunohistochemistry...some antibodies are better suited for certain applications. An antibody that performs well in immunohistochemistry...use! You can learn more about common antibody applications in our Antibodies 101 blog series.  Resources...

  12. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...their experimental needs, and some of the common applications in the lab. Why Green Fluorescent Protein?...Re-engineering GFP to increase its range of color and application Almost as soon as its sequence was elucidated...the mutation definitions found in Table 2 may not apply. Table 1: The specific mutations comprising common...others) Neutral mutations    A multitude of applications Due to its size and ease of use, GFP and other... just scratched the surface of the potential applications for GFP. It has also been used to identify particular...

  13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...DNA into the genome is useful for a variety of applications. For example, specific tissues such as blood...pathways control this process. Additional zebrafish applications and conclusions Zebrafish are also popular outside...question, and zebrafish are not ideal for every application. Disadvantages include their duplicated (i.e....—have proven useful in a variety of research applications. Lineage tracing studies highlight how these...Contemporary zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments." Methods...

  14. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...use, functional in most species, and has many applications. That said, SpCas9 is not the only game in town... makes this molecule unique, and the various applications of Cas13a. The origins of Cas13a: An RNA cleaving...discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   Figure 1: Using Cas13a as...of nucleotides.   What are the potential applications of Cas13a given what we know about its structure...CRISPR toolbox and will continue to expand the applications of CRISPR. Since this post was originally published...

  15. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...so much better. Driven by that, I successfully applied and worked as a technical support specialist for...expertise in technical support positions. When applying for technical support positions, you should try...company. To increase your chances at successfully applying for a position, you should familiarize yourself...instances, variations of the product’s specific application instructions or “optimized steps” can be linked...customers may tell all sorts of details about the application, just not the ones that matter. Therefore, if...

  16. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...decades. Widely used across various research applications, they have become indispensable tools for manipulating...-lasting changes in cells. However, for some applications, integration might be considered a double-edged... certain types of clinical or basic research applications. Considerations for IDLV use If you’re reading...Integrase-defective lentiviral vectors: progress and applications. Gene Therapy, 17(2), 150–157. https://doi.org...lentiviral vector: prospects for safe clinical applications. PeerJ, 10, e13704. https://doi.org/10.7717/...

  17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...performed on one register at a time. If a particular application makes it difficult to isolate single registers...state read-out method may not be suitable for applications that require minimal cellular perturbation. ...contains the register. Depending on the desired application, the inducible promoters we used to drive recombinase...program to find the one that best suits your application. How efficiently do RSMs work? We tested a two-input...anticipate that, depending on the context and application, some inducible recombinase systems may work ...

  18. Finding Your Science Policy Path

    Type
    Blog Post
    ...pursue your interests in policy or advocacy as a supplement to your career through engagement with your local...Project for some example proposals and consider applying for their accelerator for some hands-on guidance...This may be more challenging if you are trying to apply for an outright job in a field where you have no...specific scientific credentials.  But before you even apply to that first science policy job or fellowship, ...connect you with alumni before and during your application process so you can learn more! Figure ...

  19. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...immunoprecipitation applications, but none of the traditional tags were suitable for both applications. To prove...These tags are easy to use in multiple common applications. Due to their limited effects on native protein...particularly in single-molecule or high-throughput applications. Perhaps IT5 and IT6 will even replace our older...

  20. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...suitability of a systemic capsid for an individual application is dependent on a number of factors, including...test your chosen capsid before using it in an application.     Figure 1: AAV toolkit for targeted...doses are recommended for all systemic capsid applications. For example, AAV-PHP.eB is typically administered...experimentally determine the best titer for your application.   Pro tip!Want to know if a capsid is a good...always, validate the capsid for your specific application and animal model.  Other model organisms Tropism...points to its potential utility in translational applications and in models such as human organoids. Now what...helps you find the right one to test in your application. If you think you’ve found a good fit (or if ...
Showing: 161 - 180 of 651 results