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We narrowed to 749 results for: REV

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Showing: 161 - 180 of 749 results

  1. CRISPR Plasmids - Yeast

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  3. Troubleshooting and Optimizing a Western Blot

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    Blog Post
    ...transfer worked with Ponceau S staining or other reversible protein staining immediately after your transfer...expecting low amounts of protein, make sure your reversible staining method of choice is appropriately sensitive...transfer buffers. To slow down smaller proteins and prevent them from over-transferring, increase the amount... away the SDS. To speed up larger proteins and prevent them from under-transferring, try increasing the...Technical errors Search online/ask an expert Review protocol videos for a visual guide to good technique...Check transfer with Ponceau red stain or other reversible stain* Roll out all of the bubbles in the...

  4. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  5. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...real time. Two-photon microscopy was the next revolution in cell imaging, giving researchers the ability...(intravital imaging) studies it’s enabled are revealing the cellular behaviors that mediate adaptive and...proteins (protein that undergoes light-induced irreversible photoconversion from green fluorescence to red... 1. Peters, Nathan C., et al. "In vivo imaging reveals an essential role for neutrophils in leishmaniasis.... Egen, Jackson G., et al. "Intravital imaging reveals limited antigen presentation and T cell effector...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immune cells harvested from patients and thereby prevent cancer cells from using this receptor to evade ...immunotherapies currently under study. Check out this review for an in-depth look at a variety of up and coming...monoclonal antibody-based therapeutics." Nature Reviews Cancer 15.6 (2015): 361-370. PubMed PMID: 25998715...Lloyd J. Old. "Antibody therapy of cancer." Nature Reviews Cancer 12.4 (2012): 278-287. PubMed PMID: 22437872...Cancer immunotherapy via dendritic cells." Nature Reviews Cancer 12.4 (2012): 265-277. PubMed PMID: 22437871...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...optogenetics. Annual review of neuroscience. 2011;34:389-412. doi: 10.1146/annurev-neuro-061010-113817....effectiveness of your optogenetic manipulations (though a reviewer will probably ask for electrophysiology data too...best suit your specific experiment. Consult these reviews for a discussion of the different variants [1, ...underlying brain disease in animal models. Nature reviews Neuroscience. 2012;13(4):251-66. doi: 10.1038/nrn3171...

  9. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  10. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other...

  11. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...integrate protein tags into the genomes of S. cerevisiae and S. pombe. The protocol is surprisingly simple... in yeast. These tagging vectors are based on previously described pFA6a-link vectors and include a Kan...assessed many of these fluorescent tags in S. cerevisiae, looking at their performance in categories such...a complimentary set of plasmids for use in S. cerevisiae, with the additional benefit of multiple selection...

  13. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...Design Tools CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other...

  15. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Mechanisms. Annual Review of Biophysics, 46, 505–529. https://doi.org/10.1146/annurev-biophys-062215-010822...lobes are inactive in the absence of guide RNA, preventing Cas9 from making off-target cuts.   Pro tip! ...CRISPR–Cas immunity and genome editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/...High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity. Nature...

  18. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...brain-visceral organ signaling. The problem  Previously, there were very few tools available for monitoring...   To develop these fibers, the labs looked at previous implementation of optogenetic tools, calcium sensors...stimulation using NeuroStack and the fibers as previously described, then measured electrophysiological...optoelectronics for neural interfaces. Chemical Society Reviews, 48(6), 1826–1852. https://doi.org/10.1039/C8CS00710A...
Showing: 161 - 180 of 749 results