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We narrowed to 782 results for: SCR;

Showing: 161 - 180 of 782 results

  1. What's New in CRISPR - September 2019

    Type
    Blog Post
    ... fluorescent proteins, fusion proteins, or transcriptional regulators. In this system, Cas9 generates ... Find the CasX plasmids Drug inducible transcriptional activation and genome editing Yu Wang's lab...developed drug inducible CRISPR/Cas9 systems for transcriptional activation and genome editing by fusing Cas9...library with SpdCas9 to perform pooled CRISPRi screens to identify essential genes and phage host factors...metastasis cohorts and a prior mouse metastasis CRISPR screen. The Chen Mouse Tumor Suppressor Gene CRISPR Knockout...

  2. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench ...submission of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don...qualifications to apply for a position.  A job description is usually a wish list and the hiring manager... way to a hiring manager. Learn to confidently describe your strengths in your resume and don't be shy...

  3. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...efficiency! Screening mutant libraries After generating your mutant libraries, you have to screen thousands...generating the library of mutants and how they are screened. When conducting antibody evolution experiments...across large mutant libraries, high-throughput screening techniques such as ELISA and cell surface display... on the surface of cells. This enables direct screening via flow cytometry for improved binding under ... for Protein Engineering: library generation, screening, and affinity maturation. Methods in Molecular...

  4. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...sequence prior to an ATG start codon to prevent transcription in cells where the recombinase was not present... transgene into the correct orientation for transcription. Upon recombination one of lox2272 sites will...expression. To test their hypothesis, they randomly scrambled one pair of lox sites and either used the same...transgene (homologous pairs) or used different scrambled sequences to flank the transgenes (non-homologous...amplification. On the left homologous pairs of scrambled cre-recombinase sites lead to greater frequency...leaky expression. On the right non-homologous scrambled recombination sights lead to reduced reversion...

  6. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...make the screen-sharing experience more interactive. The button is on the right of the Share Screen control...control panel on the top of the screen and every participant get to annotate things as they come up in their...their minds. The final screen can also be saved and exported as a picture. I believe that showing something...pivotal in writing publications/grants too. As described by several works (Wuchty et al., 2007, Vicens ...

  7. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...of Amsterdam. The previous post in this series described a practical approach to selecting a bright fluorescent...literature are performed in different ways. We describe a number of issues with some of the experimental...fluorescence, which, in its simplest form, can be described by a mono-exponential decay (analogous to radioactive...). Consequently, the intensity decay is often described by the t½, which is the time after which half ... al, 2008; Bindels et al, 2017) and cannot be described by a single parameter. Therefore, it is necessary...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...CRISPR pooled libraries that allow functional screening of thousands of genes in genome-wide experiments...dividing and non-dividing cells. More detailed descriptions of the lentiviral elements and the 2nd and 3rd...regulation, gene of interest expression, knockout, and screening. There are many more useful viral vectors available... lab. Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral...collection can be used for in vivo phenotypic screening of small-molecule libraries and can be combined...

  9. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...or gene copy. The coding sequence for GFP is transcribed and translated along with the gene, producing... last exon of the gene to ensure it would be transcribed and translated. For an N-terminally tagged protein...each cell line. The editing strategy and design described above can be used to create similar donor plasmids...Endogenous gene tagging with fluorescent proteins as described here enables live cell imaging to study cellular...

  10. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...user wants to carry out a high-throughput genetic screen, it is expensive and time consuming to individually...each insertion. Also, it remains challenging to screen for the genetically modified strains that contain...simultaneously introduce a selection marker for screening modified strains. With SapTrap, the user first...selectable marker (floxed Cbr-unc-119) for easy screening of the insertion event, and a variety of connector...hundreds of genes, generating powerful genetic screening libraries. Additional protein tagging systems ...

  11. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...template with the endogenous gene. Here, we'll describe the techniques and the plasmids used to inactivate... a rare event, so you must select as opposed to screen for cells where recombination has occurred. Neomycin...Conditional knockouts The methods and plasmids described in the preceding sections are simple ways of knocking...knockout, researchers often use the Cre/Lox system described earlier. In this case, you design your targeting... this gene will still be expressed. You'd first screen for this specific recombination outcome using PCR...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...nickase system is based on the Cas9 D10A nickase described in Figure 4 of the Cong et al. (2013) Science ... The human U6 promoter prefers a 'G' at the transcription start site to have high expression, so adding...co-transfect in HR donor. Alternatively, a new system described recently is the double nickase system, with PX335...destroy the BbsI sites, a double digest should discriminate between positive and negative clones. Clones..., how can cells with that mutation be selected/screened? A13: Before starting your experiment, consider...sequencing, for example) on each colony in order to screen for positive ones.  -- OR -- If your HR template...

  13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...If you’re new to Rett Syndrome, this blog post describes the history of Rett Syndrome research, the role...MECP2 in Rett Syndrome Rett Syndrome was first described in 1966 by Austrian physician, Andreas Rett, who...maturation (Tillotson & Bird, 2019) and is a global transcriptional regulator of thousands of genes. Studies have...have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...groove of AT-rich DNA sequences to regulate transcription (Baker et al., 2013). Mutations that affect ...

  14. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...you can use to study these genes! Groucho, a transcriptional co-repressor The first conference room, that...Addgenies, was named after the protein Groucho, a transcriptional co-repressor important in development. Groucho.... It binds to a specific set of DNA-binding transcription factors to repress the expression of genes primarily...Ish-Horowicz. "The Groucho/TLE/Grg family of transcriptional co-repressors." Genome biology 9.1 (2008): ... Jiang, Jianqiao, and Helen White-Cooper. "Transcriptional activation in Drosophila spermatogenesis involves...

  15. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ...developed a separate script for manually inputting known innocuous differences. This script compares the sequence...disregards the specified discrepancies. Any sequences that are a perfect match from this script can then continue...time, but does not leave much flexibility for discrepancies in backbone sequences. Most theoretical sequences...

  16. Hot Plasmids - November 2023

    Type
    Blog Post
    ...in vitro and in vivo. Incorporating reverse transcriptase (RT) variants, PE6a and PE6b are 500–800 bp ...LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID, where two proximity labeling enzymes...recently created a system for recording the transcriptional history of a cell. Lin et al. generated a fusion... HaloTag dyes and showed they could record transcriptional activation events as bands of induced GFP-iPAK4...Analyzing the ticker tape images could recover transcriptional histories, accurate to within 40 minutes. ...

  17. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...specialized strains are available to confer greater transcriptional control, assist with proper protein folding...Lacks functional RNaseE which results in longer transcript half-life F- ompT lon hsdSB (rB- mB-) gal dcm...addition of IPTG. T7 RNAP is then available to transcribe the gene of interest from a T7 promoter on the... the expression plasmid in order to repress transcription from a hybrid promoter. Although the DE3/T7 ...don't see protein overexpression? The strains described above should generate sufficient expression levels...

  18. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...Jennifer and I spent about an hour that morning scrambling. Everything we had set up involved multiple people...people in tight locations. Conference rooms for on-screen presenters! Labs and benches! Everything was out...videos. It’s really nice to have a scientist on-screen, somebody who really knows the protocol and can...together a video from their recorded answers. No scripting made this a lot easier and I didn’t need to be...where I could focus on camera and audio, and the on-screen talent could just focus on their interview or performance...

  19. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...genes from a single transcript, or if you want to prevent read-thru transcription from resulting in the...3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated from a designed repeat protein...protein combinatorial library by in vivo functional screen." Scientific reports 5 (2015). BIOFAB plasmid ...the production of a downstream peptide, the transcription terminators in this series are just the tool...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...therapeutics - one using dCas9-SAM to activate HIV-1 transcription and destroy infected cells, the other using ...histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV-1 genome. For a targeted...dCas9-VP64 fusion commonly used to activate transcription, but with two additional activation domains ...domains (MS2 and p65) to enhance transcriptional activation. Zhang et al. designed multiple gRNAs to target ...to Use CRISPR Pooled Libraries for Genome-Wide Screens Additional Resources onf Addgene.org Check ...
Showing: 161 - 180 of 782 results